Immunotherapies, such as checkpoint inhibitor drugs, have made worlds of difference for the treatment of cancer. Most clinicians and scientists understand these drugs to act on whats known as the adaptive immune system, the T cells and B cells that respond to specific threats to the body.
New research from an international team co-led by George Hajishengallis of the University of Pennsylvania School of Dental Medicine suggests that the innate immune system, which responds more generally to bodily invaders, may be an important yet overlooked component of immunotherapys success.
Their work, published in the journal Cell, found that training the innate immune system with -glucan, a compound derived from fungus, inspired the production of innate immune cells, specifically neutrophils, that were primed to prevent or attack tumors in an animal model.
The focus in immunotherapy is placed on adaptive immunity, like checkpoint inhibitors inhibit the interaction between cancer cells and T cells, says Hajishengallis, a co-senior author on the work. The innate immune cells, or myeloid cells, have not been considered so important. Yet our work suggests the myeloid cells can play a critical role in regulating tumor behavior.
The current study builds on earlier work published in Cell by Hajishengallis and a multi-institutional team of collaborators, which showed that trained immunity, elicited through exposure to exposure to the fungus-derived compound -glucan, could improve immune recovery after chemotherapy in a mouse model.
In that previous study, the researchers also showed that the memory of the innate immune system was held within the bone marrow, in hematopoetic stem cells that serve as precursors of myeloid cells, such as neutrophils, monocytes, and macrophages.
The team next wanted to get at the details of the mechanism by which this memory was encoded. The fact that -glucan helps you fight tumors doesnt necessarily mean it was through trained immunity, says Hajishengallis.
To confirm that link, the researchers isolated neutrophils from mice that had received the innate immune training via exposure to -glucan and transferred them, along with cells that grow into melanoma tumors, to mice that had not received -glucan. Tumor growth was significantly dampened in animals that received cells from mice that had been trained.
To further support this link between myeloid precurors and the protective quality of trained immunity, the scientists performed bone marrow transplants, transferring bone marrow cells from trained mice to untrained mice that had been irradiated, effectively eliminating their own bone marrow.
When challenged later, the mice that were recipients of bone marrow from trained mice fought tumors much better than those that received bone marrow from untrained mice.
This is innate immune memory at work, said Technical University Dresdens Triantafyllos Chavakis, a long-term collaborator of Hajishengallis and co-senior author of the study.
The experiment relied on the memory of bone marrow precursors of neutrophils of the trained donor mice, which were transferred by transplantation to the recipient mice and gave rise to neutrophils with tumor-killing ability.
The researchers found that the antitumor activity likely resulted from trained neutrophils producing higher levels of reactive oxygen species, or ROS, than did untrained neutrophils. ROS can cause harm in certain contexts but in cancer can be beneficial, as it acts to kill tumor cells.
Looking closely at the myeloid precursors in the bone marrow of trained animals, the team found significant changes in gene expression that biased the cells toward making neutrophils, specifically a type associated with anti-tumor activity, a classification known as tumor-associated neutrophils type I (TAN1).
Further investigation revealed that these changes elicited by innate immune training cause an epigenetic rewiring of bone marrow precursor cells, changes that acted to make certain genes more accessible to being transcribed and also pointed to the Type I interferon signaling pathway as a likely regulator of innate immune training. Indeed, mice lacking a receptor for Type I interferon couldnt generate trained neutrophils.
-glucan is already in clinical trials for cancer immunotherapy, but the researchers say this finding suggests a novel mechanism of action with new treatment approaches.
This is a breakthrough concept that can be therapeutically exploited for cancer immunotherapy in humans, Hajishengallis says, specifically by transferring neutrophils from -glucan-trained donors to cancer patients who would be recipients.
Hajishengalliss coauthors on the study were Penn Dental Medicines Xiaofei Li; Technical University Dresdens Lydia Kalafati, Ioannis Kourtzelis, Tatyana Grinenko, Eman Haga, Anupam Sinha, Canan Has, Marina Nati, Sundary Sormendi, Ales Neuwirth, Antonios Chatzigeorgiou, Athanasios Ziogas, Pallavi Subramanian, Ben Wielockx, Peter Mirtschink, Kyoung-Jin Chung, Mathias Lesche, Andreas Dahl, Panayotis Verginis, Ioannis Mitroulis, and Triantafllos Chavakis; University of Bonns Jonas Schulte-Schrepping, Joachim L. Schultze, and Mihai G. Netea; Biomedical Research Foundation of the Academy of Athens Aikaterini Hatzioannou; DFG-Center for Regenerative Therapies Dresdens Sevina Dietz; Max Planck Institute of Molecular Cell Biology and Genetics Antonio Miguel de Jess Domingues and Ian Henry; and Max Planck Institute of Biochemistrys Peter Murray.
Kalafati and Kourtzelis were co-first authors and Hajishengallis, Verginis, Mitroulis, and Chavakis were co-senior authors.
George Hajishengallis is the Thomas W. Evans Centennial Professor in the Department of Basic and Translational Sciences in the University of Pennsylvania School of Dental Medicine.
The study was funded by the European Research Council and the National Institutes of Health (grants DE024716, DE026152, and DE28561).
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