Pamplin Media Group – The anatomy of a wildfire response – Pamplin Media Group

Wildland firefighters in places like Southern Oregon work grueling hours and face sometimes-brutal conditions.

Last month's Game Hog Creek Fire, located 22 miles northwest of Forest Grove, burned just under 200 acres in a three-week span.

But while relatively harmless by wildfire standards it did not grow explosively, and it burned in a remote and unpopulated area its presence, coupled with Oregon's tragic fires over the past 11 months, has raised awareness of the dangers of these natural disasters, along with appreciation for firefighting work and the people who do it.

Dave Luttrell, who has spent 26 years working for the Oregon Department of Forestry fighting fires and who presently runs the South Fork prison camp nestled in the middle of the Tillamook State Forest, is keenly aware of the complexities that come with wildfires. They can start quick, move quicker, and regardless of the experience of those fighting them, they can be very dangerous.

"You have to be concerned with safety first and foremost, because people die," Luttrell said bluntly. "We don't lose too many people, thankfully, but while we have safety officers on crews, we basically say everybody on the fire is a safety officer and tell them that if there's something they're not comfortable with, we encourage them to say something."

Those crews often come from everywhere. The Bootleg Fire in Southern Oregon which has burned more than 400,000 acres since igniting more than a month ago boasts more than 2,000 firefighters. Like many Oregon wildfire responses, many of those battling the fire work for the Department of Forestry. They also get a crucial assist from contractors, an oft-underappreciated component of wildfire response.

Brandon Knox, who lives in Bend, flies helicopters for Leading Edge Aviation. Knox spends half of his year fighting fires. He was based in Chiloquin and working the area when he and his spotter laid eyes on what would become the Bootleg Fire. As per protocol, they flew over and assessed the scene before calling it in. As they did, and as they waited for a response, they witnessed the just how quickly wildfires can progress.

"There wasn't any real water supply nearby, so we just circled it," Knox said. "When we initially got over the top of it, it was probably about a half-acre. Within an hour, it grew to 200 or 300 acres, and by the time we went back for the night, which would've been about six hours, it was close to 3,000 acres with 60-, 70-foot flames."

When he's on wildfire duty, Knox's job consists of scooping and dropping water, placing personnel in nearby locations, and helping those in charge eyeball the blaze from above. He normally works 10 days on, two days off, and typically 7 a.m. to midnight.

An average morning starts with a tour of the blaze with strategic personnel who are looking for the best angles of attack. Knox and his team will do that two to three times daily, providing vital intelligence to firefighters on the group. The remainder of his day will be spent doing pretty much everything else.

While he's now based in Oregon, Knox estimates he's worked in more than a dozen states, and he's been doing so for more than a decade.

And just because he's not on the ground doesn't mean his job isn't dangerous. Knox and other pilots on wildfire response have smoke, heat, varying altitudes, wind and trees to deal with not to mention the fire itself.

"You're constantly managing your power with the different winds and the turbulence, and with the added weight of the water or the amount of people you're carrying," Knox said.

He said there's an "oh, s--t" moment almost every day.

"When you're close to the top of a mountain or maybe the top of a ridgeline, the winds are always going to be shifting," Knox said. "And when you're out there with a long line or doing bucket work and that wind shifts, you often want to go one way, and the helicopter wants to go the other. There's always something."

And the ground has its own share of complexities. Luttrell said when Department of Forestry crews get called to a fire, they have to find or construct housing for hundreds to thousands of crew members, provide necessary supplies, and, of course, feed firefighters on the scene.

Fire crews work 12-hour shifts, either day or night. They start with a breakfast, take a lunch with them, then return and eat, and go to bed in preparation to do it all over the following day.

"It's hard work," Luttrell said. "There's not a ton of downtime, and these guys are dealing with a lot."

Luttrell said there are a number of ways to attack a fire, and technique often depends upon the size of the fire and the terrain in which it's burning. They'll do different things for grass fires, different things yet for fires consisting of fuels such as brush or oak savannas, and then if the terrain doesn't allow for trucks to get on site, they'll have to get crews directly into the area to attack it by hand as they did with the Game Hog Creek Fire, which largely burned in rugged slash lands.

"They often do what they call flanking maneuvers," Luttrell said. "They never try to take a fire head-on. They start at the back end, where it's not burning as hot, and they go around the edge."

Homes in the area can complicate things as well. Oftentimes, wildfires burn in remote areas of the state, like the Tillamook State Forest. When there are homes in the area, though, protecting them is a top priority as it was for firefighters battling blazes in Washington and Clackamas counties last September.

Luttrell said that starts with prevention. The Department of Forestry and other fire agencies tell homeowners to keep wood piles separate from the house, clear brush and trees so as to keep them a certain distance from structures, and keep flammable objects away from the house.

If an area with many structures is at risk, the Office of the State Fire Marshal typically gets involved, freeing up more resources to keep the fire at bay or, at least, help save what they can.

"We definitely don't want to see anybody's house burned down," Luttrell said. "So, if there's something we can do, we will, but we also won't put firefighters at risk."

Luttrell said there is increasing concern about powerlines. Downed lines were the culprit behind some of the most prominent fires in Oregon last September.

"Certainly, with powerlines, there's a chance they can start fires," he said, "so in times of concern, they're working with local utilities to determine if and when they should shut off power."

One common question that arises around wildfires is defining "containment," which indicates how much of the fire has been enclosed by a control line. A wildfire with 25% containment means control lines have been completed around 25% of the fire's perimeter.

Control lines sometimes called firebreaks can be created with controlled burns or by removing dry wood, brush and other vegetation with dozers. The intent is to form a barrier that will stop a wildfire from spreading, according to the California Department of Forestry and Fire Protection, which has borne the brunt of the United States' increasingly dangerous wildfires over the past decade.

And do firefighters ever really get these things out before the winter takes care of it in the end?

The answer is: sometimes yes, sometimes no. But often, it depends on the weather.

Obviously, cooler and wetter conditions are beneficial, but Knox says that even when wildfires appear to wind down, the process of monitoring them for flare-ups is just beginning.

"As they get things under control and they start to release resources, we'll take somebody up to monitor if from above," he said. "They're always making sure there's no hotspots near the line and nothing that can jump containment. That's always what scares them."

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Meet the 7 Pinoy virologists who will be at heart of anatomy of soon-to-rise PH Virology and Vaccine Institute – Manila Bulletin

Balik Scientists. Balik Puso. Balik Pilipinas.

Seven Filipino virologists who were trained abroad responded to the call of the Department of Science and Technology (DOST) to be part of the first-of-its-kind Virology and Vaccine Institute of the Philippines or VIP.

As they are scheduled to pay a courtesy call on Department of Science and Technology (DOST) Secretary Fortunato Boy T. de la Pea on Friday, Aug. 13, they are expected to share their expertise in the study and development of local vaccines against the coronavirus disease (COVID-19) and their scientific researches on virology and other diseases.

It has been noted that from 1975 to 2020, the DOST was able to work with 564 Balik Scientists through 716 engagements under its brain gain flagship initiative, Balik Scientist Program (BSP).

The Balik Scientist Program has been bringing home Filipino scientists from all corners of the world for almost 46 years now, DOST Undersecretary for Research and Development Rowena Cristina L. Guevara told the Manila Bulletin.

As the Philippines continues to fight and brace itself for the effects of the pandemic, we saw the eagerness and dedication of our Balik Scientists to help our country. We wish to express our gratitude to our Balik Scientists for their unconditional and unwavering love and service to the country.

As the DOST is set to roll down its red carpet to the seven Balik Scientists, it is good to know their affiliations and expertise, putting them at the heart of the anatomy of the VIP which may rise at the New Clark Economic Zone in Capas, Tarlac by the end of 2023 or in 2024.

The profiles of the seven Filipino virologists were provided by Office of the Undersecretary for Research and Development, led by Guevara.

DR. ELPIDIO CESAR NADALA JR.

Ph.D. in Microbiology and Animal Virology

Post Doctoral in Aquatic Virology and Medical Biotechnology

-Vice President of Research and Development, Diagnostics for the Real World, Ltd (DRW), California, USA

Dr. Elpidio Cesar Nadala Jr. is a virologist and microbiologist with 20 years of experience in academic research and 17 years in the industry developing diagnostic assays for the detection of bacterial and viral pathogens.

He is the co-founder of the Diagnostics for the Real World, Ltd (DRW), where he spearheaded the development of rapid diagnostic tests for the detection of Hepatitis B Virus (HBV) surface antigen, Human Immunodeficiency Virus (HIV) antibodies, and Hepatitis C Virus (HCV) antibodies, as well as improved versions of the CE-marked Chlamydia rapid test.

His team was behind the development of the SAMBA II SARS-CoV-2 test for detection of SARS-CoV-2 RNA. The SAMBA II SARS-CoV-2 test was developed and validated within two months in 2020.

Currently, the SAMBA II SARS-CoV-2 test is used in 79 hospitals and schools in the United Kingdom with a total of 648 Assay Modules deployed and 300,000 tests used so far.

DR. LOURDES NADALA

Ph.D. in Microbiology (Major in Animal Virology)

-Vice President, Regulatory Affairs/Quality Assurance, Diagnostics for the Real World, Ltd (DRW)

Dr. Lourdes Nadala is a microbiologist/virologist by training with over 15 years of experience in microbial systematics and culture collection-related activities and over 20 years of shrimp and human diagnostics, including ISO accreditation and successful regulatory submission of in vitro diagnostic medical devices.

She spearheaded DRWs efforts for ISO 13485 accreditation and regulatory approval of in vitro diagnostic (IVD) products which include molecular point-of-care tests for early diagnosis of HIV in infants, viral load monitoring, and recently, SARS-CoV-2 Test using the SAMBA platform.

She also has extensive experience in field evaluations and clinical trials in IVDs.

She also worked on detection methods for infectious pathogens and spoilage organisms affecting food safety, ISO accreditation for 9001 and ISO Guide 34 (Reference materials), AOAC validations, and customer-driven research projects related to food safety and shelf life.

DR. TEODORO FAJARDO JR.

Ph.D. in Molecular Biology (Major in Molecular Biology and Molecular Virology

Post Doctoral Fellow at London School of Hygiene and Tropical Medicine, UK with PhD in Public Administration (Major in Public Management)

-Healthcare Scientist Team Manager , UK National External Quality Assessment Service (NEQAS)

Dr. Teodoro Fajardo Jr. has specialization in Molecular Virology and Molecular Biology (which includes but not limited to: gene cloning, virus isolation, culture, propagation, and virus plaque assay, TCID50, reverse genetics virus propagation, generating infectious RNA of (+) RNA virus creating virus progeny from in-vitro transcribed RNA, and other molecular virology techniques (in vitro RNA synthesis of full-length viral RNA, in vitro RNA transcription, cell-free and mammalian cell mRNA translation, cell-free viral assembly system, RNA-RNA interactions assay for viral RNA packaging and assembly, nucleic acid isolation and purification, site-directed mutagenesis and cloning,gel electrophoresis, viral protein isolation and analysis, western blot, in vitro and in vivo gene expression of monocistronic and bicistronic mRNA, in-vitro mRNA translation, transfection of cultured cell, viral plaque analysis, analysis of expressed viral proteins)

DR. MYRA HOSMILLO

Ph.D. in Molecular Medical Science

Post Doctoral Fellow in Professor Ian Goodfellows Lab, Division of Virology, Department of Virology

-Research Associate, Division of Virology, Department of Pathology, University of Cambridge

Dr. Myra Hosmillo is an expert in the field of virology, specifically on veterinary and medical virology with a 12-year research career largely focused on virus-causing gastroenteritis.

She first worked on animal viral gastroenteritis caused by rotaviruses, caliciviruses, hepatitis E virus, toroviruses, and coronaviruses.

She was also involved in developing experimental systems to use animal viruses as a surrogate model of human viruses; particularly established an enhanced cell culture system and reverse genetics of porcine sapovirus to study its virus replication and the interactions with host innate immune response.

In Cambridge, she developed fundamental systems to directly study the pathogenesis of human norovirus using human intestinal organoids and replicon systems in human gastric tumor cells.

DR. CHRISTINA LORA LEYSON

PhD in Infectious Diseases

-Postdoctoral Researcher, Exotic and Emerging Avian Viral Diseases Group, Southeast Poultry Research Laboratory (SEPRL)

Dr. Christina Lora Leyson is an expert in standard DNA, RNA, and protein laboratory techniques including quantitative PCR, cloning, and protein expression and knowledge on sequence analysis, phylogenetics, and homology modeling with basic coding skills using Python for applications such as data wrangling and analysis.

DR. HOMER PANTUA, DVM

Ph.D. in Biomedical Sciences specializing in Immunology and Virology

-Genentech, South San Francisco, CA, USA; BioAssets Corporation

Dr. Homer Pantua is an Innovative and motivated infectious disease drug discovery scientist with over 13 years of industry experience as the lead scientist.

He has strong technical skills that encompass microbiology, molecular biology, immunology, biochemistry, and biotechnology.

He has an excellent track record of working as a lead scientist in diverse projects that progressed from early discovery to development.

DR. LEODEVICO L. ILAG

Ph.D. in Microbiology and Immunology; Postdoctoral Research Fellow in Structural biology of viruses and viral proteins

-Molawin Creek Ventures Pty. Ltd. (Melbourne, Victoria, Australia); Xerion Ltd (Brighton, Victoria, Australia); Philippine Asian Biotechnology R&D, Inc. (Manila, Philippines); Plentex Philippines, Inc, Plentex Realty Inc, PlentexAgri-Milling Corp, Plentex Aquaculture Corp (Tacloban, Leyte, Philippines)

Dr. Leodevico L. Ilag is a veteran microbiologist and immunologist with 25 years of solid research in discovery, preclinical and clinical development of biologics and dietary/food ingredients (peptides, proteins, antibodies, natural products, agricultural waste streams) with applications in oncology, cardiovascular disease, CNS, infectious disease, metabolic disease, and dermatology.

He has technical experience in antibody engineering; structural biology; virology; immunology; biochemistry; microbiology; genetics.

He has 25 years of international (Europe, US, Asia, and Australia) entrepreneurial biotechnology experience (R&D, business development/licensing/deal-making, strategy, general management, intellectual property management, financing, and operations) from conception through seed financing to initial public offering (IPO) raising the equivalent of more than A$80 million.

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Goldsmith: "The Anatomy of a Screw Up: The Biden Eviction Moratorium Saga" – Reason

I am not alone in raising red flags about the Biden Administration's conduct with respect to the eviction moratorium. (See my prior writings here, here, here, here, here, here, here, and here). Today, Lawfare published two detailed pieces about the situation. First, Jack Goldsmith wrote a post titled "The Anatomy of a Screw Up: The Biden Eviction Moratorium Saga." Second, Alan Rozenshtein wrote a post titled "Did the Justice Department Give President Biden Legal Advice on the CDC Eviction Moratorium?" Read both of the pieces carefully. Here, I'll excerpt a few highlights.

The introduction of Jack's piece captures many of my reactions to the Biden Administration's screwup:

It is hard to fathom how the experienced policy advisors and lawyers atop the Biden administration could have screwed up so badly in connection with the administration's defense of the Centers for Disease Control and Prevention (CDC) eviction moratoriums. The administration made it seem like it was acting blatantly unlawfully, when it was not. It made it seem like it needed bottom-covering arguments from law professors outside the administration when the arguments that the Justice Department of two administrations had made in defending the moratorium ban sufficed. And it made it seem like Speaker of the House Nancy Pelosi, rather than Attorney General Merrick Garland or White House Counsel Dana Remus, was directing legal decisionmaking in the Biden administration. All of this happened because the Biden administration over-reacted to a Supreme Court order that refused to halt the CDC moratorium. The administration's rule-of-law credibility is the big loser; and the Supreme Court's shadow docket the big winner.

Jack alluded to another credibility problem. OLC and the SG recognized that they had valid arguments, but didn't want to burn credibility with the Court. The Timesreported that administration lawyers felt lucky they got a reprieve from Justice Kavanaugh.

The department, and especially the Solicitor General, might have worried about credibility before the Supreme Court in light of its June 29 order, even if the order technically had no legal consequences. Or, more powerfully, the Justice Department might have worried that a likely and perhaps quick loss on the merits in the Supreme Court on a renewed eviction moratorium might limit the CDC's ability to take vital emergency action in the future. To say that the administration had legal options to renew the eviction moratorium is not at all to say that it would be a good idea to do so. The point is simply that no legal or ethical obstacle stood in the way.

Now the SG faces the worst of all worlds. I think Jack left out the most important aspect of the credibility problem. Biden said that he expected to lose, but hoped the policy would remain in place for a month so the money could be distributed. We are teetering very close to a bad faith abuse of legal process. And the Chief will not be pleased.

Finally, Goldsmith writes how these actions give the impression that DOJ "acted lawlessly.":

These revelations, in light of the earlier administration statements, made it seem like the administration had concluded that it could not lawfully renew the CDC moratorium but flipped its legal view under ferocious political pressure from Pelosi and Bush, with the assistance of cherry-picked legal advice from professors close to the administration. It seemed, in short, like the administration acted lawlessly to satisfy the fury of the left wing of the democratic party. This impression was deepened by the left's reaction to the administration's apparent flip-flop. Representative Alexandria Ocasio-Cortez, for example, described the flip-flop as "a huge victory for the power of direct action and not taking no for an answer."

Now, let's turn to Alan's piece. OLC is in a very precarious position. The Office, which is staffed by leading law professors, was simply cast aside. After all, Pelosi said "get better lawyers!" And the President threw OLC under the bus by going to law professors outside the administration:

Taking this story at face value, the obvious question is: where was the Justice Department in all of this? What was the position of the Office of Legal Counsel (OLC), which would ordinarily be the last word on high-profile, complex legal questions such as this one, or the Office of the Solicitor General and the Civil Division, which have responsibility for defending the new moratorium in court?

There are presumably three options. The first is that the Department of Justice told the White House that the CDC did not have the authority to issue a new eviction moratorium and the White House ignored that advice. The second option is that the department wasn't consulted, either because of an oversight from the White House or because the White House, suspecting that the department would return an answer it didn't want, simply didn't ask the Department of Justice. The third option is that the department was consulted, told the White House that the CDC did have this authority, and this fact has simply not been disclosed in the White House's public messaging so far. More reporting is needed on this question, but it's notable that, when Politico's Josh Gerstein asked Attorney General Merrick Garland whether the department signed off on the eviction moratorium, Garland did not answer the question.

Alan concludes:

If Pelosi really did tell Biden to "get better lawyers" and Biden responded by going outside the Department of Justice, that should set off alarms about the confidence that Biden has in the department's traditional role as the main source of legal advice and analysis for the executive branch.

There will be much more fallout from this incident. People in DC who are burned tend to talk to the press. And the Supreme Court will not quickly forget about this incident.

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Goldsmith: "The Anatomy of a Screw Up: The Biden Eviction Moratorium Saga" - Reason

Stem Cell Scientists Explore the Latent Regenerative Potential of the Inner Ear – SciTechDaily

The organ of Corti, the hearing organ of the inner ear, contains rows of sensory hearing cells (green) surrounded by supporting cells (blue). Credit: Image by Yassan Abdolazimi/Segil Lab/USC Stem Cell

Scientists from the USC Stem Cell laboratory of Neil Segil have identified a natural barrier to the regeneration of the inner ears sensory cells, which are lost in hearing and balance disorders. Overcoming this barrier may be a first step in returning inner ear cells to a newborn-like state thats primed for regeneration, as described in a new study published inDevelopmental Cell.

Permanent hearing loss affects more than 60 percent of the population that reaches retirement age, said Segil, who is a Professor in the Department of Stem Cell Biology and Regenerative Medicine, and the USC Tina and Rick Caruso Department of Otolaryngology Head and Neck Surgery. Our study suggests new gene engineering approaches that could be used to channel some of the same regenerative capability present in embryonic inner ear cells.

In the inner ear, the hearing organ, which is the cochlea, contains two major types of sensory cells: hair cells that have hair-like cellular projections that receive sound vibrations; and so-called supporting cells that play important structural and functional roles.

When the delicate hair cells incur damage from loud noises, certain prescription drugs, or other harmful agents, the resulting hearing loss is permanent in older mammals. However, for the first few days of life, lab mice retain an ability for supporting cells to transform into hair cells through a process known as transdifferentiation, allowing recovery from hearing loss. By one week of age, mice lose this regenerative capacityalso lost in humans, probably before birth.

Based on these observations, postdoctoral scholar Litao Tao, PhD, graduate student Haoze (Vincent) Yu, and their colleagues took a closer look at neonatal changes that cause supporting cells to lose their potential for transdifferentiation.

In supporting cells, the hundreds of genes that instruct transdifferentiation into hair cells are normally turned off. To turn genes on and off, the body relies on activating and repressive molecules that decorate the proteins known as histones.In response to these decorations known as epigenetic modifications, the histone proteins wrap the DNA into each cell nucleus, controlling which genes are turned on by being loosely wrapped and accessible, and which are turned off by being tightly wrapped and inaccessible. In this way, epigenetic modifications regulate gene activity and control the emergent properties of the genome.

In the supporting cells of the newborn mouse cochlea, the scientists found that hair cell genes were suppressed by both the lack of an activating molecule, H3K27ac, and the presence of the repressive molecule, H3K27me3.However, at the same time, in the newborn mouse supporting cells, the hair cell genes were kept primed to activate by the presence of yet a different histone decoration, H3K4me1.During transdifferentiation of a supporting cell to a hair cell, the presence of H3K4me1 is crucial to activate the correct genes for hair cell development.

Unfortunately with age, the supporting cells of the cochlea gradually lost H3K4me1, causing them to exit the primed state. However, if the scientists added a drug to prevent the loss of H3K4me1, the supporting cells remained temporarily primed for transdifferentiation. Likewise, supporting cells from the vestibular system, which naturally maintained H3K4me1, were still primed for transdifferentiation into adulthood.

Our study raises the possibility of using therapeutic drugs, gene editing, or other strategies to make epigenetic modifications that tap into the latent regenerative capacity of inner ear cells as a way to restore hearing, said Segil. Similar epigenetic modifications may also prove useful in other non-regenerating tissues, such as the retina, kidney, lung, and heart.

Reference: 30 July 2021, Developmental Cell.DOI: 10.1016/j.devcel.2021.07.003

Additional co-authors of the study include Juan Llamas, Talon Trecek, Xizi Wang, andZlatka Stojanovain the Segil Lab at USC, and Andrew K. Groves at Baylor College of Medicine.

Sixty percent of this project was supported by federal funding from the National Institute on Deafness and Other Communication Disorders (R01DC015829, R01DC014832, T32DC009975, F31DC017376). Additional funding came from the Hearing Restoration Project at the Hearing Health Foundation.

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Rewiring cell division to make eggs and sperm | Penn Today – Penn Today

To create eggs and sperm, cells must rewire the process of cell division.

Mitosis, the common type of cell division that human bodies use to grow everything from organs to fingernails and to replace aging cells, produces two daughter cells with the same number of chromosomes and approximately the same DNA sequence as the original cell. Meiosis, the specialized cell division that makes egg and sperm in two rounds of cell division, creates four granddaughter cells with new variations in their DNA sequence and half as many chromosomes in each cell. Meiosis uses most of the same cellular machinery as mitosis to achieve this very different outcome; only a few key molecular players prompt the rewiring from one type of division to another.

One such key player is the protein Meikin, which is found exclusively in cells undergoing meiosis. New research from Penns Michael Lampson and Jun Ma, collaborating with Whitehead Institute Member Iain Cheeseman and graduate student Nolan Maier, demonstrates how Meikin is elegantly controlled and sheds light on how the protein acts to serve multiple roles during different stages of meiosis.

The findings, which appear in the journal Developmental Cell, reveal that Meikin is precisely cut in half midway through meiosis. Instead of this destroying the protein, one half of the molecule, known as C-Meikin, goes on to play a critical role as a previously hidden protein actor in meiosis.

It was a pleasure working together to understand how some of the specialized meiotic functions that are necessary for making healthy eggs and sperm are controlled, says Lampson, a professor of biology in the School of Arts & Sciences.

Cells have this fundamental process, mitosis, during which they have to divide chromosomes evenly or it will cause serious problems like cancer, so the system has to be very robust, Maier says. Whats incredible is that you can add one or two unique meiotic proteins like Meikin and dramatically change the whole system very quickly.

During both mitosis and meiosis, sister chromatidscopies of the same chromosomepair up to form the familiar X shape of a chromosome. In mitosis, each chromatideach half of the Xis connected to a sort of cellular fishing line ,and these lines reel the chromatids to opposite ends of the cell, where the two new cells are formed around them.

In the first round of division in meiosis, however, the sister chromatids stick together, and one whole X is reeled into each new cell. Meikin helps to achieve this different outcome by ensuring that, while the chromosomes are being unstuck from each other in preparation for being pulled apart, each pair of sister chromatids stays glued together in the right place. Meikin also helps ensure that certain cellular machinery on the sister chromatids is fused so that they will connect to the same line and be reeled together to the same side of the cell.

More specifically, when chromosomes are first paired up, they are glued together by adhesive molecules in three regions: the centromere, or center of the X, where Meikin localizes; the region around the center; and the arms of the X. In the first round of meiosis, Meikin helps to keep the glue in the region around the center intact so the sister chromatids will stick together. Simultaneously, Meikin helps prime the center region to be unglued, while a separate process unglues the arms. This ungluing allows the chromosomes to separate and be prepared for later stages of meiosis.

Cheeseman and Maier initially predicted that Meikins role ended after meiosis I, the first round of meiotic cell division. In meiosis II, the second round of cell division, the cells being created should end up with only one sister chromatid each, and so the chromatids must not be kept glued together. Maier found that near the end of meiosis I Meikin is cleaved in two by an enzyme called Separase, the same molecule that cleaves the adhesive molecules gluing together the chromosomes. At first, this cleavage seemed like the end of Meikin and the end of this story.

However, unexpectedly, the researchers found that cells lacking Meikin during the second half of meiosis do not divide properly, prompting them to take another look at what happens to Meikin after it gets cleaved. They found that Separase cleaves Meikin at a specific point--carving it with the precision of a surgeons scalpelto create C-Meikin, a previously unknown protein that turns out to be necessary for meiosis II. C-Meikin has many of the same properties as the intact Meikin molecule, but it is just different enough to take on a different role: helping to make sure that the chromosomes align properly before their final division.

Theres a lot of protein diversity in cells that you would never see if you dont go looking for it, if you only look at the DNA or RNA. In this case, Separase is creating a completely different protein variant of Meikin than can function differently in meiosis II, says Cheeseman, who is also a professor of biology at the Massachusetts Institute of Technology. Im very excited to see what we might discover about other hidden protein forms in cell division.

Answering the question of Meikins role and regulation throughout meiosis required a close collaboration and partnership between Maier and Lampson lab senior research scientist Ma, the Lampson lab being experts on studying meiosis using mouse models. Working with mouse oocytes, or immature egg cells, Ma was able to reveal the behaviors and critical contributions of Meikin cleavage in meiotic cells in mice. Both labs credit the close exchange with helping them to get a deeper understanding of how cells rewire for meiosis.

Once cells have completed these specialized meiotic divisions, the researchers found that it was critical for oocytes to fully eliminate Meikin. They determined that, after meiosis II, C-Meikin is degraded by another molecule, the anaphase-promoting complex or APC/Cthis time for good. With Meikin gone and the rewiring of cell division reversed, eggs and sperm are ready for mitosis; should they fuse and form an embryo, that is the next cell division they will undergo. The researchers note that the way Meikin is regulated by being broken downfirst into C-Meikin and then completelymay help cells to organize their timing during meiosis. Breaking apart a protein is an irreversible step that creates a clear demarcation between before and after in a multi-step process.

The researchers hope that by uncovering the intricacies of meiosis, they may shed light on what happens when the creation of eggs and sperm goes wrong and perhaps contribute to the understanding of infertility.

Written by Greta Friar

The study was supported by grants from The Harold G & Leila Y. Mathers Charitable Foundation, the National Science Foundation (Grant 2029868), a Pilot award from the Global Consortium for Reproductive Longevity and Equity, the National Institutes of Health (GM126930 and GM122475), and the Henry and Frances Keany Rickard Fund Fellowship from the MIT Office of Graduate Education.

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Rewiring cell division to make eggs and sperm | Penn Today - Penn Today

Growing demand for Cell Culture-based Vaccine to propel the global Cell Culture Market – BioSpace

In molecular and cellular biology, cell culture acts as a significant tool for biological research. Today, most biotechnology products are primarily reliant on the mass culturing of animal cell lines. Cell cultures have found applications in diverse areas and serve as a model system for numerous research efforts.

An increase in funding from the government for cell-based research is significantly triggering the growth of the global cell culture market. In addition to this, cell culture techniques are widely used as an alternative to current egg-based strategies for the development of cell-based vaccines. With an increase in communicable diseases and the risk of a pandemic, cell culture has potential applications in the development of viral vaccines. Thus, cell culture technology is been extensively used in the development of U.S-licensed vaccines such as vaccines against rubella, smallpox, chickenpox, hepatitis, rotavirus, and polio. All such factors coupled with the growing prevalence of chronic diseases leading to a surge in demand for monoclonal antibodies and growing use of single-use technologies are escalating the growth of the global market.

Growing demand for cell culture products including equipment, reagents, and cell culture medium from academic and research institutes is fuelling the growth of the market. Moreover, the growing demand for 3D cell culture, major players focusing to expand the business in untapped regions, and the launch of new cell cultures are strengthening the growth of the global market. In March 2021, Thermo Fisher Scientific announced launch of new Gibco Human Plasma-like Medium (HPLM). It mimics the metabolic profile of human plasma and is the first cell culture medium that is especially designed to enable the researchers to get a realistic view of cell growth in the human body. Furthermore, growing demand for Covid-19 vaccine due to the rapid spread of deadly coronavirus will generate ample opportunities for the growth of the global market during the forecast period.

The global cell culture market is segregated based on product, application, end-user, and region. The product segment is bifurcated into consumables and instruments. The application segment is divided into stem cell technology, tissue engineering & regenerative medicine, drug screening & development, cancer research, and others. The end-user comprises pharmaceutical & biotechnology companies, research institutes, and others.

Regionally, North America is estimated to dominate the global cell culture market during the forecast period. This is majorly due to increase in cell culture-based vaccine approvals, growing demand from research institutes, and the growing prevalence of chronic diseases. Asia Pacific is predicted to grow rapidly during the forecast period. This is attributed to the increase in government funding for research purpose and the growing demand for cell culture products.

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Thermo Fisher Scientific, Becton, Dickinson and Company, Lonza Group AG, Danaher Corporation, GE Healthcare, Merck KGaA, Sartorius AG, HiMedia Laboratories, Eppendorf, and Corning Incorporated are some of the prominent players that are operating in the global cell culture market.

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Growing demand for Cell Culture-based Vaccine to propel the global Cell Culture Market - BioSpace

Astellas and Minovia Therapeutics Announce Strategic Collaboration for Novel Mitochondrial Cell Therapy Programs – Business Wire

TOKYO & HAIFA, Israel--(BUSINESS WIRE)--Minovia Therapeutics, Ltd. (CEO; Natalie Yivgi-Ohana, Ph.D., Minovia) and Astellas Pharma Inc. (TSE: 4503, President and CEO: Kenji Yasukawa, Ph.D., Astellas) today announced a worldwide strategic collaboration and license agreement for the research, development, and commercialization of novel cell therapy programs for diseases caused by mitochondrial dysfunction.

Through this strategic collaboration, Astellas and Minovia aim to accelerate the creation of allogeneic mitochondrial cell therapy programs. The two companies will jointly research cell therapy program candidates comprised of cells derived from Astellas proprietary genetically-engineered, induced pluripotent stem cells and augmented with Minovias proprietary MAT platform technology. The goal of these programs will be to treat diseases caused by mitochondrial dysfunction, through the transfer of healthy mitochondria to restore the patients tissues.

Minovia is a leading company in the field of mitochondrial cell therapy that utilizes mitochondrial transfer to deliver healthy mitochondria to a patients diseased cells. Minovia has a unique technology platform called Mitochondrial Augmentation Therapy (MAT), where the patients own cells are isolated, loaded with healthy mitochondria obtained from a healthy donor, and then re-infused back into the patient. Minovia is currently conducting research, development and clinical studies with MAT in mitochondrial diseases.

Astellas is engaged through its US subsidiary companies, Astellas Institute for Regenerative Medicine (AIRM) and Universal Cells Inc., to advance allogeneic, off-the-shelf, differentiated cell therapy programs derived from pluripotent stem cells1. The new collaboration with Minovia extends Astellas capabilities in mitochondrial biology, and follow its recent acquisition of Mitobridge, Inc. and Nanna Therapeutics Limited2,3.

We are excited and honored to collaborate with Astellas, stated Minovias Co-founder and Chief Executive Officer, Natalie Yivgi-Ohana, Ph.D. We share with Astellas both their passion for mitochondrial science and their commitment to patients in need of new therapies. As Minovia continues the development of Mitochondrial Augmentation Therapy, we believe this partnership is critical to accelerate the development of off-the-shelf, allogeneic cell therapy programs for the many patients living with mitochondrial diseases caused by mitochondrial dysfunction.

Naoki Okamura, Representative Director, Corporate Executive Vice President, Chief Strategy Officer and Chief Financial Officer, at Astellas said, "We, at Astellas, have positioned mitochondrial biology as one of the Primary Focuses of our research and development strategy to develop therapies for patients with unmet medical needs. One of the aspirations of this Primary Focus is to establish a mitochondrial cell therapy platform. Minovia is pioneering mitochondrial cell therapy and has unique technologies for enhancing delivery of healthy mitochondria to the patients cells. This strategic collaboration with Minovia will accelerate and expand our pipeline of treatment options for patients with diseases with underlying mitochodrial dysfunction.

Under the terms of the agreement, Minovia receives an upfront cash payment of $20M USD. Through the joint research program with Minovia, if Astellas develops and commercializes product candidates for diseases caused by mitochondrial dysfunction, Minovia is eligible to receive up to $420M USD per product in future development, regulatory and commercial milestone payments from Astellas.

*1: R&D Meeting (December 10, 2020). Available at: https://sw4503.swcms.net/ja/ir-library/ir-meetings/inframe/main/014/teaserItems1/07/linkList/0/link/RDmeeting2020_pre_jp.pdf *2: Astellas Corporate website Accelerating the discovery and development of novel drugs that target mitochondrial functions.. Available at: https://www.astellas.com/jp/en/stories/science/mitobridge *3: Astellas Corporate website Primary Focus - Mitochondria Biology. Available at: https://www.astellas.com/jp/en/partnering/primary-focus#Mitochondria-Biology

About MinoviaMinovia Therapeutics, Ltd. is a clinical stage company and the first to use a mitochondrial cell therapy approach with the hope of bringing life-changing therapies to patients living with mitochondrial diseases, through their proprietary Mitochondrial Augmentation Therapy (MAT) platform. Minovia has an on-going autologous cell therapy program in clinical development, utilizing MAT for the future potential treatment of primary and secondary mitochondrial diseases. For more information, please visit our website at https://www.minoviatx.com.

About AstellasAstellas Pharma Inc. is a pharmaceutical company conducting business in more than 70 countries around the world. We are promoting the Focus Area Approach that is designed to identify opportunities for the continuous creation of new drugs to address diseases with high unmet medical needs by focusing on Biology and Modality. Furthermore, we are also looking beyond our foundational Rx focus to create Rx+ healthcare solutions that combine our expertise and knowledge with cutting-edge technology in different fields of external partners. Through these efforts, Astellas stands on the forefront of healthcare change to turn innovative science into value for patients. For more information, please visit our website at https://www.astellas.com/en.

Cautionary NotesIn this press release, statements made with respect to current plans, estimates, strategies and beliefs and other statements that are not historical facts are forward-looking statements about the future performance of Astellas. These statements are based on managements current assumptions and beliefs in light of the information currently available to it and involve known and unknown risks and uncertainties. A number of factors could cause actual results to differ materially from those discussed in the forward-looking statements. Such factors include, but are not limited to: (i) changes in general economic conditions and in laws and regulations, relating to pharmaceutical markets, (ii) currency exchange rate fluctuations, (iii) delays in new product launches, (iv) the inability of Astellas to market existing and new products effectively, (v) the inability of Astellas to continue to effectively research and develop products accepted by customers in highly competitive markets, and (vi) infringements of Astellas intellectual property rights by third parties. Information about pharmaceutical products (including products currently in development) which is included in this press release is not intended to constitute an advertisement or medical advice.

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Astellas and Minovia Therapeutics Announce Strategic Collaboration for Novel Mitochondrial Cell Therapy Programs - Business Wire

Researchers Find Molecular Switch That Regulates Fat Burning in Mice – Newswise

Newswise BOSTON Linked to serious health problems including cancer, diabetes and cardiovascular disease, obesity affects more than a third of adults in the United States. Presently, there are few safe and effective nonsurgical therapeutic interventions available to patients with obesity.

Now, a multi-disciplinary team of researchers has demonstrated that a metabolic regulatory molecule called Them1 prevents fat burning in cells by blocking access to their fuel source. Led by microscopy experts at Beth Israel Deaconess Medical Center (BIDMC) and metabolism experts at Weill Cornell Medicine and NewYork-Presbyterian, the study may contribute to the development of a new type of obesity treatment. The teams findings were published June 9 in Nature Communications.

To help explain how the protein Them1 turns off heat production, BIDMCs cell biology and microscopy expert, Susan Hagen, PhD, associate vice-chair for research in the Department of Surgery at BIDMC, and Yue Li, PhD, a postdoctoral researcher in her laboratory, used light and electron microscopy to observe Them1 in action in mouse brown fat cells grown in the laboratory.

Them1 is an interesting molecule, said Hagen. If you inhibit or block its expression, metabolism increases and that reduces body weight.

The experiments showed that when the cells are stimulated to burn fat, a chemical modification causes Them1 molecules to spread out, or diffuse, throughout the cell. This frees the cellular powerhouses called mitochondria to efficiently turn the cells fat stores into energy. But when the stimulation stops, Them1 molecules quickly reorganize into a structure called a biomolecular condensate. Situated between the mitochondria and the fats they use as fuel, the condensed Them1 molecules limit energy production.

It turned out to be so incredibly interesting, said Hagen, who is also director of Microscopy and Histology Core Facilities at BIDMC and associate professor of surgery at Harvard Medical School. We asked other microscopy experts whether they had ever seen anything like the unusual images we found in resting cells. Using very sophisticated electron microscopy techniques, we were able to show for the first time, as far as we know what the bimolecular condensate looks like in electron microscopy.

The study explains a new mechanism that regulates metabolism, said David Cohen, chief of the Division of Gastroenterology and Hepatology at Weill Cornell Medicine and NewYork-Presbyterian/Weill Cornell Medical Center and the Vincent Astor Distinguished Professor of Medicine at Weill Cornell Medicine. Them1 hacks the energy pipeline and cuts off the fuel supply to the energy-burning mitochondria. Humans also have brown fat and produce more Them1 in cold conditions, so the findings may have exciting implications for the treatment of obesity.

Cohen and Hagen, both members of the Harvard Digestive Diseases Center, have been collaborators since 1983. The current study supported in part by a five-year, multi-PI grant from the National Institutes of Health also included collaborators with expertise in structural biology from Emory University.

This was the most fun I have ever had in science in my life, Hagen added. Including multiple primary investigators with different expertise gives you the power of doing things that you could never do on your own.

Co-authors included Yue Li, Samaksh Goyal, Lay-Hong Ang, and Mahnoor Baqai of BIDMC; Norihiro Imai, Hayley T. Nichols, Tibor I. Krisko of Weill Cornell; Blaine R. Roberts, Matthew C. Tillman, Anne M. Roberts, and Eric A. Ortlund of Emory University.

This work was supported by the National Institutes of Health (R01 DK 103046, R01 DK0488730 and NIHT32DK007533), the Harvard Digestive Disease Center (P30 DK034854) and the National Institutes of Health shared-instrumentation grant program for the High Pressure Freezer (S10 OD019988-01), the Pinnacle Research Award from the AAASLD Foundation, Weill Cornell Department of Medicine Pre-Career Award, and an American Heart Association Postdoctoral Fellowship, and a Research Science Institute/Center for Excellence in Education Summer Research Fellowship.

The authors declare no competing interests.

About Beth Israel Deaconess Medical Center Beth Israel Deaconess Medical Center is a patient care, teaching, and research affiliate of Harvard Medical School and consistently ranks as a national leader among independent hospitals in National Institutes of Health funding. BIDMC is the official hospital of the Boston Red Sox. For more information, visitwww.bidmc.org.

Beth Israel Deaconess Medical Center is a part of Beth Israel Lahey Health, a health care system that brings together academic medical centers and teaching hospitals, community and specialty hospitals, more than 4,000 physicians and 35,000 employees in a shared mission to expand access to great care and advance the science and practice of medicine through groundbreaking research and education.

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Researchers Find Molecular Switch That Regulates Fat Burning in Mice - Newswise

New study shines light on how plant cells perceive and respond to mechanical forces – News-Medical.net

Minuscule tunnels through the cell membrane help cells to perceive and respond to mechanical forces, such as pressure or touch. A new study in the journal Science is among the first to directly investigate what one type of these mechanosensitive ion channels is doing in the tip-growing cells in moss and pollen tubes of flowering plants, and how.

Biologists led by Elizabeth Haswell at Washington University in St. Louis discovered that so-called PIEZO channels are not found along the plasma membrane in plant cells as they are in animal cells.

Instead, they observed that PIEZO channels have retreated into the plant cell, an unexpected discovery. PIEZO channels are found deeper within the cell, in the membranes of vacuoles -- the large, intracellular organelles that help maintain cell turgor and fulfill a number of other roles in the plant cell.

PIEZO channels in plants play a dramatic and critical role in regulating the shape of the vacuole and how much membrane there is."

Elizabeth Haswell, Professor of Biology in Arts & Sciences and a Howard Hughes Medical Institute-Simons Faculty Scholar

"This is the first example of PIEZO channels involved in regulating organelle morphology," she said. "The data we present could lead to new lines of investigation for both plant and animal PIEZO homologs."

As the name suggests, mechanosensitive ion channels are paths, or tunnels, through cell membranes that respond to mechanical forces. Under certain forces a channel opens, allowing the flow of ions across the membrane.

In humans, PIEZO channels are essential for life; without them, cell development halts. They are recognized for their role in perceiving light touch, shear force and compressive force. Dysfunction in PIEZO channels has been linked to multiple human diseases.

PIEZO channels were first identified in plant genomes in 2010. After a decade of research on animal homologs, this new research shines a spotlight on plant cells and explores how they differ from animal cells. Other research teams have recently shown that PIEZO channels are involved in mechanical sensing in plant roots.

The researchers made their initial discoveries using the tip-growing cells of a somewhat atypical model plant, spreading earthmoss (Physcomitrium patens).

But the scientists were able to extend their findings beyond moss to cells from other distantly related plants, including in pollen tubes in a classic model, the flowering plant Arabidopsis thaliana.

"Mosses are one of the groups that comprise the bryophytes, which are the second largest land plant lineage," said Ivan Radin, a research scientist in the Haswell laboratory and first author of the new paper.

"When we can show that the same thing happens both in moss and a flowering plant, as we did here, the most likely conclusion is that the process is ancestral -- it's at least as old as the land plants are," Radin said, noting that land plants colonized Earth about a half a billion years ago.

Radin became the Haswell laboratory's de facto moss specialist with coaching from co-author Magdalena Bezanilla, a professor of biological sciences at Dartmouth University. Bezanilla previously worked with Washington University's Ralph Quatrano, emeritus dean and the Spencer T. Olin Professor Emeritus of Biology, who was an early adopter of moss.

"The more time passes, the more we love it," Radin said. "Moss proved to be an exceptionally good model."

As a next step in this research, scientists in the Haswell laboratory are now conducting additional experiments to show how external and internal forces directly affect PIEZO channels in moss cells.

"Plant PIEZO channels are likely to be controlled by membrane tension in plants the same way they are in animals," Haswell said. The scientists are also exploring the evolution of these channels in algae.

Now they know where PIEZO channels are found in the cell, Haswell and her team are poised to find out what these proteins are doing in the vacuoles.

"We are looking at how PIEZO channel activation results in membrane elaboration and how it is regulated," Haswell said. "We want to know how the localization evolved and what it does in other cell types. We plan to compare and contrast the structure and function with the animal channels and in organisms across the green lineage."

Source:

Journal reference:

Radin, B., et al. (2021) Plant PIEZO homologs modulate vacuole morphology during tip growth. Science. doi.org/10.1126/science.abe6310.

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New study shines light on how plant cells perceive and respond to mechanical forces - News-Medical.net

A single dose of Covishield vaccine induced high neutralizing antibody and T cell responses – Sri Lankan study – nation.lk – The Nation Newspaper

A study in Sri Lanka has found that 93.4% individuals developed antibodies to a single dose of the Covishield vaccine, the made-in-India variant of Oxford-AstraZenecas AZD-1222 formulation, while 97.1% developed neutralizing antibodies.

With the initiation of the COVID-19 vaccination program, Health Care Workers in Sri Lanka were the first to be vaccinated with Covishield vaccine.

Therefore, the Allergy, Immunology and Cell Biology Unit, Department of Immunology Molecular and Molecular Medicine investigated antibody responses to the SARS-CoV-2, neutralizing antibody responses, antibody responses to the variants, along with T cell responses.

The full article is available here at Nature Communications, which has an impact factor of 14.9:Immune responses to a single dose of the AZD1222/Covishield vaccine in health care workers | Nature Communications

93.4% individuals developed antibodies to a single dose of the vaccine and 97.1% developed neutralizing antibodies. There was no difference in immune responses in younger individuals compared to those >60 years of age.

The vaccine induced potent T cell responses with 67.1% developing T cell responses. However, the antibody responses to the beta SARS-CoV-2 virus variant was low. Antibodies to delta was not measured, as delta was not a problem during the time the study was conducted (February and March 2021).

The research team included scientists from the Allergy, Immunology and Cell Biology Unit, Department of Immunology Molecular and Molecular Medicine, Colombo Municipality Council and University of Oxford.

The funding for this study was provided by the World Health Organization, UK Medical Research Council and the Foreign and Commonwealth Office and the Chinese Academy of Medical Sciences (CAMS) Innovation Fund for Medical Science (CIFMS), China.

The group is also studying immune responses to other vaccines such as Sputnik, Moderna and Sinopharm and how the immunity to these different vaccine persist and compare with natural infection.

The most recent preprint on the immune responses to the Covishield vaccine at 16 weeks is available here:Immune responses to a single dose of the AZD1222/Covishield vaccine at 16 weeks in individuals in Sri Lanka | medRxiv

Source: USJ

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A single dose of Covishield vaccine induced high neutralizing antibody and T cell responses - Sri Lankan study - nation.lk - The Nation Newspaper