Biochemistry

The Different Types of Immunostaining – News-Medical.net

Immunostaining encompasses numerous techniques that are suited to a variety of different applications.

Image Credit: Jarun Ontakrai/Shutterstock.com

However, they are all methods that rely on the use of antibodies to detect and identify proteins within biological samples. They can be used to asses and identify the topographical distribution of abnormal cells, blasts infiltrates, and megakaryocytes.

The term was coined back in 1941 when it was first used to describe immunohistochemical staining. These days, immunohistochemical staining is just one of several established immunostaining techniques, including enzyme-linked immunosorbent assay, flow cytometry, immuno-electron microscopy, and Western blotting.

The techniques are commonplace in biology and molecular biology labs, and are used for a variety of applications in a wide range of fields of study, from oncology to hydrobiology.

Here, we describe the five types of immunostaining techniques.

The enzyme-linked immunosorbent assay, also known as ELISA, is commonly used in biochemistry. Developed in 1971 by Engvall and Perlmann, the method quantifies peptides, proteins, antibodies, and hormones present in a sample by immobilizing an antigen on a solid surface before it is complexed with an antibody that is associated with an enzyme.

Identification is then possible when the conjugated enzyme activity is assessed through incubation with a substrate, resulting in a measurable product.

When the physical and chemical characteristics of cells or particles are sought to be determined, flow cytometry is often the most suitable method. The technique was established in the 1950s, and over the decade's many advancements have been achieved in its methodology and equipment. Currently, measurements are made from cells in solution while they travel through the instruments laser at speeds of 10,000 cells per second. Flow cytometry offers benefits to the technicians who choose to use it, including high sample throughput speed, making it an attractive option as an immunostaining assay.

Immuno-electron microscopy, also referred to as EM immunolabelling and immuno-EM, is a technique that tags antibody molecules with electron-dense substances, usually, and most effectively, being small gold particles, which are seen during the analysis as easy to spot dark dots. The assay allows for the simultaneous detection of more than one type of molecule because particles of different sizes can be used to tag different antibodies.

The technique was first developed as a diagnostic aid that assisted in the detection and identification of viruses, such as gastroenteritis and rotavirus. Today it is still used to diagnosis a variety of viral infections. It is considered to be one of the most sensitive and rapid methods for this application.

The most common application of immunostaining is immunohistochemistry, which is used to assist in the diagnosis of various diseases, including different types of cancer. It has also shown its use in neuropathology, and hematopathology, helping in the classification of diseases in these groups and evolving criteria for their diagnosis. Another area in which it has made a significant impact is that of genetic study, where it has been used to determine the role of specific gene products, elucidating their function in vital biological processes. The technique has become invaluable to both medical research and clinical diagnostics.

The method involves selectively identifying antigens in a sample of cells within a tissue section through the principle that certain antibodies will bind to specific antigens present in the tissue. It was established back in the 1930s before it was first reported in 1941.

The initial principle outlined that antibodies labeled with a fluorescent dye could detect pneumococcal antigens in infected tissues. Since then, the technique has been developed, and new enzyme labels have been introduced, including peroxidase, alkaline phosphatase, and colloidal gold. The use of radioactive elements has also been developed for use with autoradiography.

The final immunostaining method is the Western blot method, a widely used technique that has firmly ingrained itself in the fields of cell and molecular biology. Western blot allows researchers to determine and quantify the proteins existent within a cell, identifying specific proteins out of the mixture of proteins that are present in cell samples.

There are three parts to the Western blot method, the first is separation by size, the second being transfer to a solid support, and finally, a target protein is marked using a suitable primary and secondary antibody to visualize it.

Immunostaining methods have become essential to numerous branches of scientific study, they have also become well established in various clinical applications, mostly in assisting in diagnosis as well as determining characteristics that facilitate more accurate diagnostic criteria.

Since the immunohistochemistry technique was first reported on in 1941, four further types of immunostaining techniques have emerged: enzyme-linked immunosorbent assay, flow cytometry, immuno-electron microscopy, and Western blotting. These methods are being expanded and developed on all the time, growing their use in different applications, and improving on their accuracy and reliability.

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The Different Types of Immunostaining - News-Medical.net

Anatomy

Ukrainian plane crash: the anatomy of a cover-up – The Jerusalem Post

It happened less than 10 minutes after Ukrainian Airlines flight 752 left Tehrans airport heading for Kiev on January 8, 2020. Two missiles 30 seconds apart hit the commercial jetliner destroying the aircraft and killing all 176 passengers and crew members on board.This was initially believed to be a crash due to mechanical failure. However, in light of the tense, war-like atmosphere following Irans attack on an Iraqi military base housing US forces, there was speculation from the outset that the aircraft was downed by Islamic Revolutionary Guard Corps (IRGC) missiles. Theories on the IRGCs complicity was not only advanced by Western intelligence agencies, but also by the general public; particularly members of the Iranian diaspora.Many videos taken on mobile phones began to surface on social media within hours of the incident. Some of them clearly showed objects believed to be missiles colliding with the aircraft. One of the very first pieces of evidence supporting the destruction of the jetliner by missiles was provided by an Iranian-American social activist in Northern California, Ashkan Monfared.Monfared posted on Twitter the image of an object that astoundingly resembled missile wreckage and added the following caption in Persian: This piece was found in the area of the Ukrainian Airlines plane crash that had fallen in front of a house. Does an airplane have anything like this? Is it not the tip of a missile? The image quickly went viral on social media.Almost immediately after posting his tweet, Monfared was attacked by what appeared to be a wave of pro-regime Iranian bloggers on Twitter. They accused Monfared of spreading disinformation aiming to tarnish the image of the Islamic Republic. They also dismissed the image as a hoax.Many of the accounts that attacked Monfared were based in the US or Canada and followed the approach of the National Iranian American Council, a Washington-based organization highly suspected to lobby for the Iranian regime. A seemingly coordinated effort was then made to completely silence Monfared on Twitter. Subsequently, less than 24 hours after his initial post, Monfareds Twitter account was suspended.Displaying an image of Monfareds tweet, a known Iranian regime supporter based in Canada, Hossein Derakhshan, tweeted in Persian on January 9, Almost concurrent with suspension of Ashkan Monfareds account, which of course was due to dissemination of fake news, numerous [other] Iranian accounts linked to Saudi Arabia and Israel [were also suspended].DERAKHSHAN INSINUATED that Twitter accounts suggesting the regimes complicity or showing evidence of it were Saudi or Israeli agents promulgating disinformation. His tweet raised the probability that a large, coordinated group of pro-regime individuals were reporting Monfareds account to trigger Twitters suspension algorithm.The campaign to silence Iranian dissidents seemed to have originated from within the highest echelons of power in Tehran. On January 9, Hesameddin Ashena, a senior advisor to Iranian President Hassan Rouhani, wrote a tweet effectively threatening Western-based journalists of Iranian descent to stay quiet about the tragedy. He wrote, Warning! Persian-language media outlet personnel of Iranian origin are warned to refrain from participating in the psychological warfare over the Ukrainian aircraft and working with the enemies of Iran.Ashenas warning seems to have resonated with pro-regime journalists. In spite of the growing evidence of foul play by the IRGC, pro-regime Persian and English-language media outlets remined silent, limiting their coverage to merely a plane crash due to technical malfunction.Meanwhile, many more Twitter accounts that raised the possibility of the regimes involvement were mysteriously shut down. The Twitter account of London-based British-Iranian attorney Daniel Rasteen was suspended after he wrote several tweets accusing the IRGC of shooting down the jetliner.For three days, the regime kept the details of the crash concealed. Inspectors from Ukraine as well as other countries arrived in Tehran to begin investigating the incident. Among the wreckage, they found debris similar to the object displayed by Monfared. The regime eventually and perhaps reluctantly admitted that the plane was unintentionally shot down by IRGC air defense.By this time, Monfareds Twitter account had been reinstated. As soon as it became evident that the crash was not accidental, the same individuals who had attacked Monfared began blaming the Trump administration for the mishap. To this day, some regime apologists still claim that the initial campaign accusing the IRGC, before the militant group admitted to their role in the tragedy, was merely anti-Iran propaganda.A recent New York Times article claimed the regime found itself compelled to tell the truth about the incident after President Rouhani threatened resignation. There is no evidence to substantiate this other than the authors claim that she received that information from her sources inside Iran.What did, however, force the regime to admit guilt was pressure from the international community, which was partly triggered by outpouring of visual evidence provided by Iranians inside the country and disseminated by people like Monfared. In an era where social media connects people from around the world, it is becoming increasingly difficult for totalitarian regimes and their henchmen to conceal their atrocities.Kaveh Taheri is an Iranian free-lance journalist and sociopolitical activist based in Turkey. Dr. Kamran Ayazi is an Iranian dentist, musician and sociopolitical activist based in Turkey.

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Ukrainian plane crash: the anatomy of a cover-up - The Jerusalem Post

Anatomy

The anatomy of a missed open shot – The Michigan Daily

For all the romanticized and clichd reasons to love sports, seeing a buzzer-beating 3-pointer hit nothing but net has to be near the top of the list. Its pure anticipation as the shooter pulls up and attempts to carve themselves in the history books of their program.

Thats the exact opportunity junior guard Eli Brooks had in the waning moments on Tuesday against Ohio State.

As each second escaped forever off the clock, the Wolverines were running one final play to tie the game and salvage a win at Crisler Center. Like most late-game scenarios, senior guard Zavier Simpson drove to the basket where he looked to find the open shooter.

There sat Brooks, alone in the corner with his defender desperately trying to get in position to make the stop. The junior caught the ball, rose up as he has done thousands of times and the ball clanged off the back rim.

Michigan got the look it wanted a wide-open shot in the corner from one of its best shooters and just flat out missed it, giving Ohio State a 61-58 win.

So why didnt it go in?

Not only that, why didnt all of the Wolverines wide-open shots find nylon? Michigans shooters consistently found themselves staring at an unobstructed view of the basket in its entirety a bright green light screaming at the ball handler to let it fly.

This tendency has done nothing but grow under coach Juwan Howard, whose policy since Day One has been: If youre open, make it rain.

Unfortunately for the Wolverines, theres been a drought in Ann Arbor.

I know we missed a ton of layups, Howard said. We missed a ton of open shots. We just have to finish, its that simple. We have to finish games. We have to win games at home. We started off with a pretty good run in the beginning of the season by winning some home games, and our first loss ended up being against Oregon, but we havent gotten back to that basketball that we were playing in the beginning of the year.

Perhaps no one player has struggled more to hit open looks than freshman forward Franz Wagner.

Tuesday, the freshman went just 2-for-8 from 3-point range and 2-for-12 from the floor. Thats good for a 25 percent 3-point percentage a disappointing mark to say the least.

But the German will be the first to tell you that these misfires are not the result of a mental hurdle needing to be conquered. Often times, pundits and onlookers alike suggest this could be the reason for a shooting drought. If a player is not shooting well, the thinking goes, they just must not be shooting confidently.

Its not, Im goin bad, I need to fix my shot, Wagner said. Im confident in my shot, I think you can see that. Ill continue to do that. We got good shots. A couple shots felt really good, but they didnt go in. Sometimes my wrist doesnt flex in the direction I want it to, so thats why you gotta keep working on it, but its not that Im not confident.

Rather, it could boil down to slight alterations in how the ball leaves his hands.

After a win against Nebraska on Jan. 28, Wagner who had just gone 1-for-5 from beyond the arc assured the public that the coaching staff insisted there was nothing mechanically wrong with his shot. The dang thing just needed to fall in the hoop.

I talked to the coaches, and we think theres nothing wrong with my shot, Wagner said in Lincoln. Were just gonna keep working on it, and you can ask anybody. Im trying to work on it and get my reps up, during the game and just trust it and believe that its going in.

So how does Wagner address this problem in practice?

He does not attempt to correct the trajectory of his previous miss. Say hes too strong on one of his shots and the ball pops off the back of the rim. Instead of attempting to put less muscle on his shot, Wagner holds fast in his approach, refusing to cave to the pressure that he overcorrect the amount of force put into the shot. The opposite is true for balls that fall just short of the basket.

Given the secrecy that typically surrounds players shooting mechanics, Wagners approach may be unique among his teammates.

But for someone shooting under 30 percent from 3-point land while attempting five long-range bombs a game, a change to his shooting mechanics in some form may be on the horizon. Either that, or Wagners persistence pays off and open looks may no longer be a gaping hole in Michigans offensive inefficiencies.

Because if a team cant figure out the little things in a wide-open shot over the course of a regulation game, how could it be expected to hit it with the game on the line?

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The anatomy of a missed open shot - The Michigan Daily

Anatomy

Solar-Powered Tents and Silent Game Drives The Anatomy of a Green Safari – Luxury Travel Advisor

by Sue Watt, The Telegraph, February 5, 2020

I hadnt expected to see a green mamba on a behind-the-scenes tour of Chobe Game Lodge inBotswana. Thankfully, it wasnt one of Africas deadliest snakes, but a powerful machine that crushes cans and bottles given that name by Albert Ndereki, the lodges ecotourism manager, because it is so aggressive.

The green mamba is just one of many waste management projects that has earned the lodge and Albert accolades. In May, he won the Shape Africa Innovation Award at the We Are Africa show inCape Townfor his commitment to sustainable initiatives in one of the countrys oldest lodges.

Albert joined Chobe Game Lodge as a builder in 1971. Nearly 50 years on, he takes guests on behind-the-scenes tours like mine to see his inspiring initiatives. They include producing biogas from food waste and grass cuttings, burning rubbish in incinerators and using the ash as fertiliser, and making bricks out of crushed glass bottles. Theres solar power, too, and Chobe was one of the first lodges inAfricato offer silent safaris on electric vehicles and boats.

Alberts award reflects the significance now being placed on a greener safari experience, with the emphasis on protecting a fragile environment. Our clients love to see sustainable camps, and they object when they see poor practice, says Chris McIntyre, managing director of specialist tour operator Expert Africa.

We highlight the best green camps to our travellers, but sustainability isnt the decisive criterion for choosing where to stay just yet. Factors such as location and cost can take precedence, but were sure this will change as awareness of these issues grows.

Today, leading safari operators such as African Bush Camps, Asilia Africa, Elewana, Singita and Wilderness all embrace green technology, particularly solar power: the tedious drone of a generator is rarely heard these days.

Two years ago, Singita created an off-grid renewable energy plant inSouth Africas Kruger National Park, when their Sweni and Lebombo lodges were redesigned. It was the first project in Africa to include Tesla Powerpacks for solar power storage, enabling the company to power both lodges and its entire staff villages with solar energy.

The green effect has dramatically influenced design in camps and lodges. At Asilias Namiri Plains, all decking is 100 per cent recycled plastic and walls are a local natural stone, calcrete, which helps to regulate room temperature.

Entirely covered with painted canvas, the staff village has been ingeniously constructed to resemble a classic Serengeti kopje (rocky outcrop), blending in with its surroundings. Both the camp and the staff village are completely solar-powered.

Solar-charged electric vehicles and boats are also gaining traction. Vincent Kouwenhoven, founder of niche operator Green Safaris, developedZambias first electric game drive vehicle (the eLandy) for its Ila Lodge in Kafue National Park.

I came up with the idea of an electric vehicle many years ago, he explains. As I drove from one property to another, it suddenly struck me that you only switch off a diesel engine when you encounter lions or something.

Only then do you get to enjoy the sound of the bush. This is where the idea of silent safaris was born, using EVs to enhance the customer experience. We built the very first one and started experiencing way more sightings, since most animals dont hear us coming or they are simply more at ease. The investment is substantial, but the savings on diesel and wear-and-tear definitely make it worthwhile.

Green Safaris offers solar-powered boats for sunset cruises on the Kafue river, and silent safari vehicles will feature in its new camps, Chisa Busanga in Kafue and Shawa in South Luangwa, when they open in June.

In South Africas Sabi Sand Game Reserve, the Cheetah Plains Houses has added more luxurious touches to its electric Land Cruisers, including customised suspension, heated bucket seats for chilly mornings and side-lighting on steps for easier access.

With all the well-publicised concerns about plastic, single-use water bottles are hardly ever seen on safari. Most lodges provide branded, refillable stainless-steel bottles with filtered water for game drives, which guests can take home afterwards.

Minimising the impact of safaris on wildlife is also a top priority for enlightened companies. For night drives, andBeyond Phinda Private Game Reserve in South Africa recently introduced an innovative Night Eye Experience.

High-definition infrared cameras connected to screens show guests the nights wild action in real-time viewing. The absence of spotlights means animals carry on their normal business undisturbed.

InZambia, where walking safaris were born, three walking-only bush camps opened last year, suggesting a greener, less is more approach with a lighter carbon footprint. Mapazi in South Luangwa, Tusk and Mane in Lower Zambezi and Jeffery & McKeith Safaris in Kafue are all run by renowned guides.

Instead of the sometimes incongruous luxuries associated with safaris (private plunge pools, minibars and hairdryers in vast suites), these intimate, pared-back camps have a back-to-nature vibe with exceptional guiding in exclusive wilderness areas as the main draw.

Mobile camps tread lightly on the environment, too, especially in the Serengeti, where they move to follow the Great Wildebeest Migration. Roving Bushtops has created a unique hi-tech mobile camp with solar-powered tents which fold out on to a platform, complete with a king-size bed, hot tub, flush lavatory and Wi-Fi, leaving little trace on the landscape.

I recently stayed at Wayo Africas far simpler exclusive-use mobile camp in the Serengetis Wilderness Zone, walking by day and sleeping on comfortable beds in dome tents at night. In the less-is-more mode, our bathroom tent had a bucket shower and a compost lavatory.

Wayos founder, Jean du Plessis, is a renowned guide and National Geographic TV presenter. He explained to me the importance of water conservation in Wayos approach to safari holidays.

I calculated that all these fancy camps using flush lavatories take around a million litres a day out of Serengeti water sources, he told me, adding that Wayo adds sawdust to waste to neutralise it, then mixes it with kitchen waste to make compost.

In Wayos main camps, the square tents have gutters to catch rainwater, which is then stored in black 1,000-litre bladders that are left to warm in the sun. The result? Naturally-heated bucket showers.

Every morning, I awoke to see the vast plains of the Serengeti all around me, with no one else there and a footprint so light that we were barely tiptoeing on these fragile lands. What could be greener than that?

Further information: chobegamelodge.com; expertafrica.com; africanbushcamps.com; asiliaafrica.com; elewanacollection.com; singita.com; wilderness-safaris.com; greensafaris.com; cheetahplains.com; andbeyond.com; surefootsafaris.com; tuskandmane.com; jefferymckeith.com; bushtopscamps.com; wayoafrica.com

This article was written by Sue Watt fromThe Telegraphand was legally licensed through theNewsCredpublisher network. Please direct all licensing questions to[emailprotected].

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Anatomy

‘The Rhythm Section’ | Anatomy of a Scene – The New York Times

new video loaded: The Rhythm Section | Anatomy of a Scene

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Hi, Im Reed Morano, and Im the director of The Rhythm Section. In this sequence, our main character, Stephanie Patrick whos played by Blake Lively, is out on her first job. And it sort of goes completely awry. And she ends up inadvertently in a car chase she didnt plan for. The reason why I wanted to do the movie overall is the same reason why I wanted to shoot the car chase this way. And that was because its sort of like a POV driven story. The scariest place for me after watching dozens and dozens of cinemas best car chases was from within the car. And any time Ive watched one and we cut out of the car, I feel like the tension dropped for me. Something about being in the car and having this limited visibility and having the camera bring us to what we need to see at the front or the back or at the character makes you kind of feel like youre in the seat next to her, which is the last place I think anyone in the audience would want to be. Because shes not a superhero. Shes not an action hero. Shes not a real assassin. Shes just this regular woman. In order to see the necessary things, in order to make the audience feel what shes feeling, we were going to have to coordinate really particularly between what Blake was doing in the car as Stephanie and what was happening outside in the front versus what was happening in the back or the side of the car. And Sean Bobbitt, my DP, was super excited about this as well. So what happened was was we had this old Merc, this old Mercedes. And this was the tiniest car ever. And Sean Bobbitt is a really big guy. But they took out all the seats on the passenger side and built like a slider, like a rail system, with a little seat that he would sit on. But he could slide back and forth. But he was also secured in other ways. But he had mobility to kind of be up front by the window. He could pan towards Stephanie. And he could also pan towards the back window. But he could also slide really far back when he needed to get another view. Part of the reason why the chase is so successful is not only because of all the coordinated efforts of all the stunt people were happening at the right time. It was also Blake carries the scene, and her energy is changing, you know, up and down and throughout like an emotional roller coaster. And shes really making it fun and scary to be in a car with her.

Recent episodes in Anatomy of a Scene

Film directors walk viewers through one scene of their movies, showing the magic, motives and the mistakes from behind the camera.

Film directors walk viewers through one scene of their movies, showing the magic, motives and the mistakes from behind the camera.

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'The Rhythm Section' | Anatomy of a Scene - The New York Times

Anatomy

Focus on the FaceHands-On Learning of Surface Anatomy; One-day Workshop Wednesday, April 1 – The Know

Aestheticians, cosmetologists, dental surgeons, portrait artists, and anyone who wants to learn more about the anatomy of the human face are invited to attend a special day-long workshop on Wednesday, April 1.

Focus on the FaceHands-on Learning of Surface Anatomy will be held at the non-profit Anatomy in Clay Centers in Denver, 2201 S. Delaware St., from 9 a.m. to 4 p.m.

The class will be taught by Anatomy in Clay Learning System founder, anatomist/artist Jon Zahourek, who has taught human and animal anatomy for more than 40 years.

In this engaging workshop, the focus will be on the surface anatomy of the face the soft tissues and facial glands. The class will cover all the major muscles that allow for human expression as well as the muscles that allow for movement of the jaw and the all-important function of chewing.

Students will apply clay to a scale model ofthe human skull. Working from the inside out, students will also lay in andtrack the paths of the major arteries, glands, and cranial nerves.

The cost of Focus on the Face is $295. Registerby February 14 to receive a $30 early-bird discount. The fee includes lunch anda special guest presentation by Dr. Wendy Hartsock from Allergan MedicalAffairs.

To register, contact Mary Bahus-Meyer at800-950-5025 or MaryB@anatomyinclaycenters.org.

About Jon Zahourek

Jon Zahourek has taught human anatomy for more than 40 years. He created the Anatomy in Clay Learning System during his tenure at Parsons School of Design in New York, teaching students anatomy by building it with clay on the scale model skeletons he developed. He has taught workshops in comparative primate anatomy at the Smithsonian Institution, and in comparative mammalian anatomy at the Darwin Centre/Natural History Museum in London. Zahourek trained the Smithsonian Institutions forensic facial reconstruction specialist, Betty Pat Gatiliff, who taught Zahoureks approach for decades across the United States.

About the Anatomy in Clay Centers

Thenon-profit Anatomy in Clay Centers seeks to make anatomy accessible to all, through providing educationalclasses to the public at large, including schools and organizations, using akinesthetic approach to human anatomy and zoology.

The Anatomy in Clay Centers is located one block south of the Evans Light Rail Station at 2201 S. Delaware St.

More: http://www.anatomyinclaycenters.org/

Call 720-570-7820 or email info.aicc@anatomyinclaycenters.org.

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Focus on the FaceHands-On Learning of Surface Anatomy; One-day Workshop Wednesday, April 1 - The Know

Anatomy

Anatomy of the Chiefs’ Super Bowl comeback: Patrick Mahomes’ big throw, the momentum and the victory – Sporting News

MIAMI GARDENS, Fla. Kendall Fuller could not help but smile as he stared at his phone, sitting by himself at his locker and scrolling through themessages he had received. Roughly an hour had passed since the 24-year-old Chiefs cornerback sealed Kansas City's 31-20 win over San Francisco in Super Bowl 54 with an interception.

Fuller's pick, though, arrivedwith a little more than a minute to play and with the 49ers in desperation mode after giving up 21 unanswered points in the game's final 6 1/2 minutes. He told Sporting News the moment felt "amazing," but he felt the game was won well before he beat Ninersreceiver Deebo Samuel to Jimmy Garoppolo'sdeep pass down the middle of the field.

"They hit that deep ball to 10," Fuller said when asked whether a specific play led the Chiefs to believe they could erase a 20-10 deficit in the fourth quarter, referring to quarterbackPatrick Mahomes' 44-yard strike to Tyreek Hill at the 7:13 mark. "When they hit that, that was it."

MORE:How Patrick Mahomes turned worst nightmare intoChiefs' Super Bowl dream

This is why Mahomes was named Super Bowl 54 MVP despite his throwing a pair of costly interceptions and posting a passer rating of 78.1, his second-lowest of the season. For the same reason Kansas City players believed they could stage comebacks in their two playoff games, they kept faith when down a couple scores late in the Super Bowl. That reason is thepasser Kansas City drafted with the 10th overall pick in 2017.

"He was encouraging us, telling us to believe," Hill said of Mahomes after the game. "He (saw)it in some guys' eyes, they were getting down, including myself. I was like, Man, how are we going to pull this off? And he was like, '10, youve got to believe, brother. Like the same faith youve had all of your career, youve got to believe right now.'

"He brought the guys together, and you saw what happened, man."

What happened was the play that catalyzed an effort that madethe Chiefsthe first team in NFL history to win three games after trailing by 10 or more points in a single postseason; a sequencethat madeMahomes, 24, the youngest QB ever to win Super Bowl MVP.

Said Kansas City offensive coordinator Eric Bieniemyin a conversation with SN after the game: "That's all we needed was just a spark."

The play design, simply called "Wasp," was nothing new. In fact, Mahomes said it was the same play the Chiefs ran in the second quarter of their loss to thePatriots in last season's AFC championship game.

"They were playing this kind of robber coverage all game long, where the safety was coming down and kind of robbing all our deep cross routes," Mahomes explained. "We had a good play call on it where we had (Travis) Kelce do a little deep studded cross. We had Tyreek getting one-on-one with that safety."

"That safety" was Jimmie Ward, and the sixth-year pro had no chance to defend Hill's angle route. To be fair to Ward, though, not many QBs can launch the ball from one 22-yard line to the opposite 22-yard line the way Mahomesdid.

Aside from Mahomes' ridiculous arm, the third-and-15play was successful for two reasons. One was the kind of protection from the offensive line that drew praise from just about every Chiefs and 49ers player who was asked about the turning point in Super Bowl 54.

"The biggest thing was we needed really good protection," Mahomes said.

MORE:Is Kyle Shanahan to blame for another Super Bowl collapse? It's not so simple

Added 49ers coach Kyle Shanahan: "He was allowed to hold onto the ball for a while." And San Francisco linebacker Fred Warner: "He had enough time to air it out, and we didnt have enough guys there to defend the play."

The other factor was Mahomes' awareness. When asked about the arm strength required to pull off such a throw,Bieniemyinstead credited a less tangible quality from which Mahomesbenefits.

"One thing Pat has done a tremendous job of since hes been here is he always keeps his eyes downfield," the 50-year-old coordinator said. "He's always looking to see exactly where guys are located, and also anticipate, when we do our scramble drill, where guys are going to be. Thats just huge. Thats just who he is."

The Chiefs cashed in on the big Mahomes-Hill connection with a touchdown four plays later, a 1-yard Mahomes pass to tight end Travis Kelce. Harrison Butker's PAT cut the 49ers' lead to three with 6:17 to play.

The signs of life would have disappeared, though, had Kansas City's defense not turned the spark into a flame.

Patrick Mahomes https://images.daznservices.com/di/library/sporting_news/50/6b/patrick-mahomes-020320-getty-ftr_1xd2cewnalb5w1dedpdypalm15.jpg?t=167277408&w=500&quality=80

In sports, momentum is just a fancy word for mentality, which is whyBieniemy'sconfident words did not fall on deaf ears when the Chiefs set out to defend the 49ers on the possession after Hill's big catch and Kelce's touchdown.

"EB, before we went out there, he said, Get us the ball and we're going to take care of the rest,'" linebackerDamien Wilson said.

A 5-yard Raheem Mostert run and a pair of Garoppolo incomplete passes later, San Francisco's Mitch Wishnowskywas in for his second punt in two drives.

"Sure enough," Wilson added, "it was as simple as that just putting the ball in the offense's hand."

The 44-yard catch by Hill was the Chiefs' longest from scrimmage Sunday, but Sammy Watkins' 38-yard catch on their next series was just as explosive, especially considering Watkins beat Pro Bowl corner Richard Sherman to make it happen.

According to Next Gen Stats, Mahomes' strike to Watkins marked the second "deep completion" Sherman had given up in two games after allowing zero in the regular season.

This would become the "tape don't lie" moment of the game.

MORE: 10 incredible stats from Chiefs' comeback, 49ers' collapse

"I just knew it was one-on-one from watching film," Watkins explained when asked about the play, referring to the NFC title game a couple weeks ago, when the 49ers beat the Packers. "I just thank (Green Bay receiver)Davante Adams, because I saw him kill (Sherman) on the inside release. Those are just things we worked on, and me and Pat connected and it was one of the biggest plays in the game."

Added a visibly frustrated Sherman, simply: "He made a play."

Three plays later, the Chiefs found the end zone again. Damien Williams took Mahomes' pass five yards for a score, which stood upon replay review.

Butker's PAT gave Kansas City a four-point lead with 2:50 to play. San Francisco had plenty of time and all three timeouts, but the momentum the Chiefs had built was too strong. The 49ers managed to reach their opponent's 49-yard line before Garoppolo was sacked by Frank Clark on fourth-and-10.

The Chiefs got the ball back, and two plays later, Williams was back in the end zone with a 38-yard run.31-20, Chiefs.

The spark Mahomes and Hill created had turned into an inferno that consumed the NFC champions.

Mahomes was surprised to see Fuller clinch the second Super Bowl title in Chiefs history, especially since the fourth-year corner had nearly picked off Garoppolo on the 49ers' previous possession but dropped the ball.

"I always joke with him that he never catches the interception, but he's always there," Mahomes said. "He went up and got that thing. He made a great play."

In a way, it was fitting that Fuller outjumped Samuel, of all players, to win the game considering the 49ers wide receiver had steadily produced though the first three quarters. Samuel even set arecord with 53 rushing yards, the most by a receiver in Super Bowl history. It was a microcosm of Kansas City tendingto start slowly before reminding its opponent of its superiority.

"That team, thats kind of how they've been all year," Shanahan said. "They get a little bit hot and cold. They can score very fast. That team doesnt do it every single drive, but it was a matter of time."

MORE:Jimmy Garoppolo has plenty of time, talent to correct a lazy Super Bowl narrative

Shanahan, of course, was the offensive coordinator with the Falcons when Atlanta blew a 28-3 lead and lost to New England in Super Bowl 51. The social media world was quick to reference that defeat as his 49ers collapsed Sunday. This one, though, was not a matter of second-half clock management or play-calling.

After the Chiefs scored to make it20-17, Garoppolo completed just two passesand the 49ers managed just 37 yards of offense. They simply got overpowered by what suddenly became an unstoppable force.

"The last thing you're thinking about when you're up three points and there is that much time left, the clock is not an issue at that time, especially with the timeouts," Shanahan said. "The issue was moving the chains. If you move the chains, then you will wind the clock."

Added Garoppolo: "It wasn't anything crazy. We just didn't make the plays when we had opportunities to."

With all due respect to Garoppolo, he's wrong.

The Chiefs scoring 21 unanswered points in a little more than six minutes to win the Super Bowl and to do it after fighting through a state of dejection, believing in a QB who was playing the worst game of his career was pretty damn crazy.

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Anatomy of the Chiefs' Super Bowl comeback: Patrick Mahomes' big throw, the momentum and the victory - Sporting News

Anatomy

New Book, The Anatomy of Accomplishment: Your Guide to Bigger, Better, Bolder Business Results, Addresses Unique Business Challenges of Today and…

Erin Joy curates authors for new book featuring insight from top female business executives.

ST. LOUIS, Feb. 5, 2020 /PRNewswire-PRWeb/ --Erin Joy, Founder and CEO of Black Dress Circle, a business consulting and coaching organization that supports emerging and evolving women business owners, has announced the release of a new book, titled The Anatomy of Accomplishment: Your Guide to Bigger, Better, Bolder Business Results. The book includes a compilation of stories and advice written by a variety of leading female entrepreneurs and executives from around the country. With insight from experts in multiple industries, the book addresses how to achieve success in a crowded and competitive marketplace. A book launch party will take place on March 6, two days before International Women's Day, at the Piper Palm House in St. Louis from 6-8pm.

"I am thrilled to have brought such a diverse group of women together to tell these stories which will have an impact on so many entrepreneurs and executives," says Erin Joy. "This is a must-read for anyone who wants to take their business, team, or department to the next level with improved operations, marketing, mindsets, efficiencies, and more."

Black Dress Circle specializes in creating and facilitating roundtables of female entrepreneurs and executives. An accomplished advisor and coach, Erin's roundtables have helped guide and support countless women as they address the specific challenges and opportunities that come with owning and leading companies and teams.

The book takes the roundtables' topics and tells them from the perspective of leaders across the globe. Authors include business executives and CEOs, career coaches, accountants, and more.

Early reviews have been positive. Award-winning coach and author of Quiet Mind Epic Life, Matthew Ferry says, "Bravo! There are so many gems in this book. These 19 interesting and unique female entrepreneurs offer us sage advice that I wish I had at the beginning of my own journey. That said, each of their stories has inspired me to become a better version of myself today."

A must-read for men and women business leaders, The Anatomy of Accomplishment: Your Guide to Bigger, Better, Bolder Business Results will be available to order on amazon.com and everywhere books are sold beginning March 9, 2020.

The book launch party on Friday, March 6 will include light bites and beverages, distribution of signed copies, and networking with the authors. A panel discussion with authors will begin at 6:30 p.m. Tickets are $19 for the public and free to media personnel.

About Black Dress Circle Black Dress Circles are monthly, facilitated, results-driven roundtables for female entrepreneurs. There are two variantsBDC Emerge and BDC Evolve. Both provide a forum to discuss issues, frame decisions, and share experiences with other women who understand the unique challenges of managing emerging and evolving companies. Erin Joy created this peer-directed, proven model to address the common challenges faced by women leading dynamic and developing organizations. For more information, visit blackdresscircle.com.

SOURCE Erin Joy

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New Book, The Anatomy of Accomplishment: Your Guide to Bigger, Better, Bolder Business Results, Addresses Unique Business Challenges of Today and...

Anatomy

Anatomy Of A Shootout At Jamia – The Navhind Times

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SAEED NAQVI

HAQEEQAT-e-Abadior the eternal truth of the nationwide protests will only be energised by thePolice Zindabad shouting country pistol shooter who fired into a protestoutside Jamia Millia Islamia, not far from the peaceful Shaheen Bagh that I hadleft behind just an hour ago. A row of policemen, arms folded, or leaning ontheir lathis must have been disappointed by the pistol-mans poor marksmanship.He hit a student on his wrist. But expected TV channels went to town, which wasthe purpose.

Away from this nasty distraction (which only shows up thepresent regime for what is) a much bigger reality is unfolding, on an epicscale: the entire Indian Opposition is being bypassed by the biggest protestssince Independence led by women, students and youth, ostensibly against theCitizenship (Amendment) Act, the national register of citizens and the nationalpopulation register but, as they gather momentum, the whole establishmenttrembles.

History will record the Jamia Millia as the point ofignition for this avalanche which, in a sense, completes a circle. Threebrilliant students Dr Zakir Hussain, Dr Abid Hussain and Prof Mohammad Mujeeb met in Germany and decided to join Jamia Millia to enlarge the reservoir ofenlightened, progressive Muslims in the national movements. In todays BJPparlance they would be called the tukde-tukde gang or urban Naxals. Itwould have been difficult to foist Pakistan on them because that country hadnot been formed then. In fact, opposition to the two-nation theory was anarticle of faith with this batch.

In that enlightened stream were Anwar Jamal Kidwai andShahid Mehdi. Prof Mushirul Hasan had seen the worst of Jamia as its provice-chancellor, when he was beaten up by university goons linked to a Congressleader who was riled over Prof Mushirs stand on banning books.

Rajiv Gandhi had banned Salman Rushdies SatanicVerses. Prof Mushirs position was that books should be challenged, ignoredbut not banned. There must be something good in the system that Arjun Singh, asHRD minister gave Prof Mushir (by now elevated as vice-chancellor) more freedomthan any vice-chancellor since has ever had. The result was a mushroom growthof centres named after unlikely figures: Noam Chomsky, Edward Said, Mir AnisHall and Mir Taqi Mir hall, M F Hussain Art Gallery and so on. Who knows, themovement may put fresh life into all of this.

Prof Mushir explained his Left trajectory in terms ofJamias origins as an institution of the enlightened against colonialism andimperialism. Bringing about course corrections on that count (as has beenattempted) ran the risk of being sucked into communal and identity politics.Peoples issues are overlooked. This is the dilemma of todays liberals face:they cannot give up on capitalism (therefore imperialism) even in itspost-globalisation, post-2008 avatar. They often find themselves standing withthe powerful establishments which redirect popular resentment againstinequality, for instance, towards issues of immigration, ethnic identities. Soyou have Viktor Orban in Hungary, Matteo Salvini in Italy, Marine Le Pen inFrance, Nigel Farage in the UK and kindred souls elsewhere including JairBolsonaro, the Pinochet copycat in Brazil. Since Prime Minister Narendra Modihandpicked him to be the chief guest at the Republic Day parade, it must beassumed that Modi probably nurses him as a model. This lot is clearly what theprotests are arrayed against.

The eager-beavers looking for a suitable and urgentoutcome have not noticed that the movement has already altered the scene. Amovement that can provoke stalwarts of the BJP into such glorious absurdities,deserves a trophy. Union Law Minister Ravi Shankar Prasad said, Shaheen Baghprotest is offering a platform to tukde-tukde gang. Delhi should have noplace for such people. Parvesh Sahib Singh Verma, BJP MP in Delhi, has goneone better. What happened in Kashmir with Kashmiri Pandits could happen inDelhi also. Lakhs of people enter Shaheen Bagh. They will enter houses, rapeand kill our sisters and daughters.

He sends a shiver down the spine with his threat. If theBJP comes to power on February 11, you will not find a single protester withinan hour. And within a month we will not spare a single mosque built ongovernment land.

Anurag Thakur, Minister of State for Finance andCorporate Affairs, is even more inspiring: Desh ke ghaddaron ko, he exhorts,the crowd. On cue comes the response, goli maro sa***n ko (shoot thebastards). This goes on for minutes. If a peaceful nationwide movement candrive the BJP to such reckless intemperance, it has clearly achieved a greatdeal. Above all, this shaky behaviour has been aggravated by the protestscoming so soon after reversals in Madhya Pradesh, Rajasthan, Chhattisgarh,Jharkhand, Maharashtra and, God forbid, Delhi too? Union Home Minister AmitShah will not give up on his incantation of revenge politics easily.

Another great achievement of the protests has been thesecularisation of the Muslim ghetto. The docile, homebound Muslim woman has forthe first time being seen in her public avatar, articulate, dignified,involved. The hijab and the bindi are mingling an elegant sight.

This is not a simple phenomenon. It is not without asociological readjustment within the family and community. Across communities,it has promoted a new bonhomie. Batla House, Jamia Nagar and Jama Masjid arethat much more accessible because sheer exposure of different communities in acommon cause has helped remove cobwebs of an uninstitutionalised apartheid.

The Opposition will never be in a position to takeadvantage so long as its national parties hold onto their respective obsessions the Congress urge to revive nationally and the Communist urge to revive in WestBengal. These aspirations will remain road blocks. Yes, Congress presidentSonia Gandhi may be able to sing a nicer swansong if she could somehow revivethe spirit of 2004 when the Left was not an anathema. That plus a commitment tofederalism will work. Soft Saffron has no future. IANS

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Anatomy Of A Shootout At Jamia - The Navhind Times

Anatomy

Greys Anatomy season 16 Netflix release date: When is Greys Anatomy on Netflix? – Express

When is Greys Anatomy season 16 on Netflix?

Fans of hit medical drama Greys Anatomy are eagerly awaiting the series being made available on Netflix.

The show is currently airing weekly on US Network ABC as viewers follow Meredith Grey (played by Ellen Pompeo) and her colleagues at the Grey Sloan Memorial Hospital.

This airs in its usual time slot of 9pm ET on the network.

The series is expected to land on US Netflix in the summer after the series has finished airing on ABC.

READ MORE:Greys Anatomy season 16: Who are Blake Simms and Hannah Brody?

Season 15 was available to watch on Netflix on June 15, 2019.

Therefore, it seems likely the latest outing will be made available around the same time in 2020.

When it is uploaded to the platform, it is expected all of the episodes will be available to stream at once.

Fans in the UK will not be able to watch the series on Netflix as it will likely only be available to watch on Sky Witness.

However, as of yet, a release date for the new season has not yet been announced.

Fans have been worried about the future of hit medical drama Greys Anatomy after a number of the original cast members have left the show.

The latest to leave was Justin Chambers who played Dr Alex Karev since the Pilot episode.

However, the series was renewed for two more seasons on network back in May 2019.

This includes season 16 and season 17 on ABC but the future of the series after this has not yet been confirmed.

Speaking at the Television Critics Association Winter Tour back in January, ABC Entertainment President Karey Burke addressed the rumours.

When asked if season 17 would be the last, she said: I hope not.

Greys Anatomy will live as long as Ellen is interested in playing Meredith Grey.

According to Deadline, she also confirmed they were currently in talks with Meredith Grey star Ellen Pompeo about the future.

Greys Anatomy currently airs on Thursday evenings on ABC.

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Greys Anatomy season 16 Netflix release date: When is Greys Anatomy on Netflix? - Express