Genetics

Canada: DNA discovery lends weight to First Nations ancestral story – The Guardian

When a woman named Shanawdithit succumbed to tuberculosis in Newfoundland nearly 200 years ago, it was widely believed that her death marked a tragic end to her peoples existence.

For centuries, the Beothuk had thrived along the rocky shores of the island, taking on a near-mythical status as descendants of the first people encountered by Norse explorers in what is now Canada. But their population was devastated by decades of starvation and diseases, and when she died in 1829, Shanawdithit was believed to be the last of her line.

New research from Memorial University, however, has found Beothuk DNA probably still exists in people alive today a discovery that would rewrite the history of the Newfoundlands early inhabitants, even as it confirms the accuracy of local First Nations oral tradition.

Weve got good evidence that we have genetic continuity from the Beothic into modern persons, said biologist Dr Steve Carr.

But while the finding would trigger a rethink for historians, the notion is not surprising to local Indigenous groups.

Mikmaq oral history has long asserted a shared ancestry with the original inhabitants of Newfoundland, and local First Nations have worked closely with Carr to help lend genetic evidence to their own traditions.

There were always connections or friendly relations going back more than 200 years ago and when you mingle that way, periodically, things would happen, said Chief Misel Joe of the Miawpukek Mikamawey Mawiomi, a Mikmaq First Nation in Newfoundland.

Historians believe the Beothuk are descended from a group that braved the ocean to cross from Labrador to Newfoundland thousands of years ago and whose distinct culture emerged around 1500 CE. At one point, as many as 2,000 Beothuk lived in communities scattered around Newfoundland.

For generations, they largely resisted and avoided relations with European settlers; the few interactions between the two were defined by violent encounters.

Early European settlements on the coast cut off Beothuk access to critical salmon and seals forcing them to move further inland where they sustained themselves on caribou before finally succumbing to starvation and disease.

But Carrs research suggests it was only a cultural extinction; their genetic legacy lives on.

In his study, Carr used DNA samples from Shanawdithits aunt and uncle Demasduit and Nonosbawsut whose skulls were taken to the Royal Museum in Scotland in 1828. After a long campaign by Chief Joes community, the remains were repatriated to Newfoundland in March.

After running samples through a genetics database, Carr was able to find his smoking gun a man in Tennessee who was genetically similar to Nonosbawsut, but had no known Indigenous ancestry.

With is only a small amount of data to work with, Carr hopes more samples will further demonstrate a connection.

Its easy to obtain the DNA sequence from somebody and you can count the number of similarities. Thats a very easy thing to do. But to reconstruct the patterns of a relationship is a very challenging problem, said Carr, adding that further research into the known movement and connections between the Beothuk and Mimaq was still required.

The findings also illustrate the way in which genetic uniqueness in this case the distinct sequence of Beothuk mitochondrial genomes can persist intact for generations. While humans share an immense amount of DNA that traces back millennia, said Carr, the intent of his research lay in teasing out the subtle and distinguishing differences between known groups.

For years, academia has ignored the oral histories of Indigenous peoples, said Chief Joe.

Academics are hard people to convince. They often have this mindset that this the way it was no matter what information we give them to the contrary, he said.

He described a frustrating experience in a land claims court, where the adjudicator suggested the Mikmaq first arrived in Newfoundland in the 1700s.

But we have an oral history of British sailors meeting our people and asking for directions. We drew them a map on birch bark. If this is the first time we had ever been on the land, how could we draw a map? said Joe.

Its convenient for government, for everyone, to ignore people who had no written history

The community is excited to keep working with Carr on further testing, said Joe, to further strengthen the evidence of shared ancestry.

This is a big thing for us, he said. But it all comes from something we already knew.

Original post:
Canada: DNA discovery lends weight to First Nations ancestral story - The Guardian

Genetics

Genes

Understanding the genetic material DNA and RNA, heredity, and variationthat's genetics. Studies in genetics focus on questions like:

Follow the links below to learn more about genetics, including recent developments, and read profiles of researchers working in this field.

Explains the role of genes in health and disease, the basics of DNA and its molecular cousin RNA, and new directions in genetic research.

Explores how computing advances are helping scientists uncover new details about diseases, drug treatments and even crimes.

During the fundamental process of mitosis, a type of cell division, dividing cells sometimes make errors while divvying up chromosomes. Understanding how this happens may help researchers develop targeted therapies for a variety of diseases, including cancer. Learn more about how NIGMS-funded research is exploring this vital, complex process.

Did you know that we have free education resources you can use from home to engage students in science? Explore our Science Education Partnership Award program projects and Pathways resources to find STEM learning opportunities for pre-K through grade 12.

NIGMS grantee and presidential award recipient Sohini Ramachandran, Ph.D., is challenging our understanding of genetic variation among human populations. In our latest blog post, she discusses her research on how the genetic composition of traits and diseases varies among populations, the value of statistical and computational work in human genetics, and what this all means for patient treatment.

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Genes

Genetics

genetics – Kids | Britannica Kids | Homework Help

In the 1850s and 1860s an Austrian monk named Gregor Mendel studied pea plants in his garden. He found that there were rules for how traits passed from one generation of pea plants to the next. The rules are the same for every plant and animal. During his lifetime no one understood how important these findings were.

In 1900 people rediscovered Mendels work. From then on, the new science of genetics grew rapidly. Scientists began to use it to help explain the theory, or idea, of evolution. An English scientist named Charles Darwin had put forth the theory in the 1850s. It describes how species adapt to their environment and how new species form.

In 1953 James Watson of the United States and Francis Crick of England discovered the structure of DNA. Their studies helped scientists understand how genes work and how they make copies of themselves.

By the mid-1970s, scientists had learned how to locate, remove, and insert specific genes in DNA. This work is called genetic engineering. By the 1990s scientists could clone animals, or produce animals that have exactly the same DNA as another animal. In 1996 researchers in Scotland produced the first clone of an adult mammala sheep. Some scientists worked toward cloning human beings. But others saw this work as dangerous and wrong.

In 2003 a team of researchers finished a project to identify and locate all the genes in all human DNA. The results will help scientists in the study of human biology and medicine.

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genetics - Kids | Britannica Kids | Homework Help

Genetics

Thought to be extinct, Beothuk DNA is still present in N.L. families, genetics researcher finds – CBC.ca

Steve Carr is a biology professor at Memorial University in St. John's. (Memorial University )

A St. John's genetics specialist has found DNA connections that link theBeothuk people to contemporary people, almost two centuries after the last known Beothuk died.

Steve Carr, a biology professor at Memorial University, says that his research also shows that the genetic material of the ancient Maritime Archaic people still exists to this day, centuries after they disappeared from placeslike Port au Choix, on Newfoundland's west coast.

Shanawdithit, the last knownmember of the Beothuk people, died in 1829. The Beothuk have long been described as extinct, but Carr says a more accurate phrasing is culturally extinct, as the science of DNA is now reshaping the story.

For years, Indigenous people in Newfoundland including the Mi'kmaq have maintained they are related to the Beothuk.

Carr, whose findings have been published in the journal Genome, says there is evidence to support that position.

"The question was whether those genetic descendants had descendants, and those descendants had descendants, and whether they persist to the modern times. And the answer from my analysis is, yes they do," Carr told CBC Radio's Newfoundland Morning.

DNA was harvested from the molars of the skulls of two well-known Beothuk persons: Demasduit, the aunt of Shanawdithit , and her husband, Nonosbawsut. Demasduit died in 1820. Nonosbawsut was killed a year earlier while he tried to prevent her capture.

In 2017, a graduate student collected the DNA from a museum in Scotland, where the Beothuk remains had been held until this March, when they were returned to Newfoundland.

The DNA was then compared with databases, and Carr said the links are clear.

"There is a living person who is genetically identical to Nonosbawsut," Carr said.

"I have actually spoken to the person and he's fascinated to find out this connection. The odd thing there is that he has been pursuing genealogy for a number of years. He can trace his maternal ancestry back five generations and there's no indications in that record of any First Nations or Native American ancestry."

There are no direct descendants of Demasduit and Nonosbawsut. The couple had an infant son, who died.

However, the family tree that included Demasduit and Nonosbawsut clearly has branches that are thriving today.

"However, where those two individuals were members of what we understand to be the last band of Beothuk, it's certainly possible that their brothers or their uncles or their grandfathers or their great-grandfathers or their great-great-grandfathers, that they had children who survived, and the modern persons that we find who are identical to or similar to Nonosbawsut and Demasduit are literally their cousins," Carr said.

"[This is] in exactly the way that we think about cousins. Cousins are people who have an ancestor in common."

Carr said it's likely members of the Beothuk people "had what we'll call friendly relationships" with other people, which led to children being born centuries agowho went on to reproduce family lines that still flourish.

Chief Mi'sel Joe of the Miawpukek First Nationwelcomed Carr's research.

"I wasn't surprised. We knew it all along," Joe said.

"But it was good to have it confirmed."

Carr, who is also the founder of a company called Terra Nova Genomics, will be working with the Miawpukek First Nation in Conne River for the next phase of an ongoing research project.

He intends to compare DNA samples from that Mi'kmaq community with the Beothuk DNA, and believes more family connections will likely be proven.

Read more from CBC Newfoundland and Labrador

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Thought to be extinct, Beothuk DNA is still present in N.L. families, genetics researcher finds - CBC.ca

Anatomy

Ahmedabad At 5000 Cases And Counting: Anatomy Of An Epidemic – Swarajya

Chief Minister Vijay Rupani sensed this might happen, and has to be credited with voicing key concerns first.

As early as 1 April, he said that over 1500 residents of Gujarat had attended the Nizamuddin Markaz. He appealed to the attendees to come forward and be tested, and warned against irresponsible acts which could harm society.

Sadly, his plea went ignored, and Ahmedabad is now at over 5000 cases. This lack of response was reflective of the socio-political issues faced by the citys Muslim community, and constitutes a vital part of this epidemics anatomy.

Part of the old citys problem lies in the absence of control.

For a long time, this function was ably shared between former Congress Chief Minister Chimanbhai Patel, and a gangster-associate of Dawood Ibrahim named Latif. But Patel died in 1994, and Latif was killed in 1997, after which, things were never quite the same.

So, while control of the citys profitable bootlegging trade continues to remain in old hands, discipline has weakened. This decline was exacerbated by the end of vote-banking in Gujarat, and the rise of the BJP, which relegated a formerly-crucial swing-vote to the margins of political obscurity.

Another part of the problem lies in the appalling lack of enlightened community leadership.

Ideally, the passing of Patel and Latif should have been followed by the rise of a new crop of community leaders, who would have built the necessary bridges to match power shifts. Instead, we have local Dariyapur MLA Gyasuddin Sheikh of the Congress Party, for example, who is emblematic of both this lacuna, and, the problems which municipal and district administrators presently face.

As a result, the big picture, and the art of compromise, have both been lost in the Sabarmati, leading to the growth of a dangerous recklessness, fuelled by imaginary woes of minority victimhood.

This is but natural, since without a voice to lead, a sane head to think, and discipline, base emotions always have a tendency to prevail over reason. Look at how Sheikhs Pavlovian response to the stone pelting at Shahpur, was to first, and instinctively, indulge in the vulgar art of monkey-balancing.

According to his statement in the press, the police were perhaps doing their duty, but since it was Ramzan, and since the shops were closed, people were going hungry and were thus forced to step out of their homes to hunt for food.

Readers should pause for a moment here, and think: if all shops are closed, if people have been asked to stay indoors, and everyone knows that, then who would step out to buy food from closed shops?

What sort of politician would use such inane illogic to justify such lunatic acts?

How difficult is it for people understand that if Muslim areas become no-go areas for health teams, then the entire city is put at grave risk? How can a citizen not care? And why throw stones at the police?

Obviously, Sheikhs statement doesnt fly, and is founded on gross untruths. There is no mass starvation in Ahmedabad, and the rioting happened just a day after the closure of shops was officially notified. No one runs out of food that quickly.

So, instead of the situation being mitigated, it has instead been exacerbated. Traders who have patiently suffered scarring financial losses, because they appreciate the gravity of the threat posed by the Wuhan Virus, now risk being forced to endure even greater losses solely because a few hotheads and politicians are acting bereft of civic sense.

Now, it is unsavoury that even an epidemic gets reduced to such needless Hindu-Muslim segregation, but if young Muslim men in the old city area of Ahmedabad will aggressively and violently challenge orders of public safety, solely because of their communal identity, and elected representatives will fuel those divides instead of aiding the administration, then the problem is a lot bigger than we think.

Indeed, if tear gas is being used to quell rioters in the midst of a critical public health lockdown, then the forecast is actually grim.

In a nutshell, this encapsulates the problems faced by the authorities; and in a sense, Ahmedabad is a microcosm of what is happening in other clusters, in other parts of the country.

Episodes like stone-pelting at Shahpur, and the concomitant spread of the epidemic, are not stray, bizarre responses to an existential threat. No. Communities are being actively instigated into violent insurrection by political forces, for no moral reason.

By this, the livelihoods of millions are being lost. And sad as it may seem to make this awful prediction, while the rest of Ahmedabad is quiescent for now, there could be a backlash to this mulishness beyond a point. Administrators should be on their guard.

So, while Amdavadis wait for things to return to normal, when they might indulge in their two pet activities making money on weekdays, and eating out on weekends once again, here is the gist of the matter:

Once epidemiology becomes geography and demographics, then the anatomy of a pandemic teaches us the physiology of its manufactured obduracy, and offers a blunt pharmacology for its treatment without clemency.

May that happen soon, peacefully.

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Ahmedabad At 5000 Cases And Counting: Anatomy Of An Epidemic - Swarajya

Anatomy

Grey’s Anatomy’s Caterina Scorsone Splits from Husband Rob Giles After 10 Years of Marriage – PEOPLE

Grey's Anatomy's Caterina Scorsone Splits from Husband Rob Giles | PEOPLE.com Top Navigation Close View image

Grey's Anatomy's Caterina Scorsone Splits from Husband Rob Giles After 10 Years of Marriage

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Grey's Anatomy's Caterina Scorsone Splits from Husband Rob Giles After 10 Years of Marriage - PEOPLE

Anatomy

The Ideal Anatomy of a Well-Stocked Liquor Cabinet – Broadsheet

Put down the boxed wine. Your favourite venues might be out of action right now, but it doesnt mean your happy hour has to suffer. With a few well-chosen bottles of spirits, some imagination and a trip to the backyard, its possible to drink well without going out.

[Right now] drinking is all about drinking better, says bartender Matt Linklater, who typically mans the bar at Black Pearl in the inner-Melbourne suburb of Fitzroy.

Linklater is working from home right now, but often ends his days in lockdown with a vermouth on ice or a vermouth and soda. On the weekends hes more likely to whip up a cocktail, or reach into the freezer for a batched Martini or Negroni.

Every Friday Ive been hosting a cocktail hour with my housemates, he says. It just breaks up the week. Last week was frozen Margaritas in the Nutribullet and the week before that was Cosmos. I dont know what this week is going to be maybe Mojitos because the weather is getting a little bit colder, so its the ultimate denial cocktail.

We asked Linklater for his tips on setting up your home bar to prepare a range of easy-to-make cocktails without shakers, strainers, muddlers, jiggers or any other tool that sounds like a Dickensian profession.

Your shopping listI always have four staples in my house, says Linklater. Gin is just super versatile. Whether you want something easy like a G&T, or something fancier like a Negroni, or a Martini. Tanqueray is a good choice because its got four botanicals, so it lends itself well to different types of drink.

He also likes to have a sipping whisky handy for late at night by the fire. Something usually from Ireland, so the smokier, peatier styles like Caol Isla the better.

Bottle three is an interesting vodka like Ketel One Citroen. Probably a great excuse for more Cosmos, says Linklater, its use of lemon and lime plays perfectly. And bottle four is Don Julio tequila because tequila is always fun, he says. I drink it on ice smooth, peppery, textural or use the Reposado in cocktails like a Tequila Espresso Martini or Old Fashioned.

Plan aheadAt the Black Pearl during service, we generally batch everything, says Linklater. And I take that sensibility home as well.

He finds batching makes the measurements more straightforward. For example, Negronis are super simple, he says. [Theyre] just three equal parts. Its all ratio.

A batch of Martinis on the dryer side is five parts gin to one part dry vermouth with a 30 per cent dilution (addition of ice). I just leave that in the freezer and its ready to go whenever I am, says Linklater.

He also has a sensible, potentially cost-saving tip for those trying at home for the first time: Make sure you have all the ingredients before you start to batch, and add everything in cheapest to most expensive, just in case you mess it up.

Mix it upWhen mixing cocktails professionally, bartenders use a range of tools. Linklater says teacups are perfectly adequate measuring devices, and you can use tupperware as a makeshift cocktail shaker.

You dont need anything really, says Linklater. I have, in the past, shaken drinks in tupperware containers [and] protein shakers. Nutribullets are great, especially for frozen Margaritas. All you really need is a bottle to pre-dilute (add ice to) a Martini or a Manhattan and throw it straight in the freezer then you dont need any equipment at all.

Another tip: Use tablespoons and teaspoons in place of a measuring cup or a jigger that youd have at the bar.

ImproviseIf you feel like getting creative, Linklater recommends experimenting with flavours and garnishes, rather than spirits.

[Trialling spirit combinations] can go really well but more often than not it goes very, very wrong, says Linklater. Even coming up with new drinks in the bar will generally take 10 to 20 attempts. Were lucky we can allow for that. But if youre at home, 10 or 20 attempts can be a whole bottle of gin you [didnt really] enjoy.

If youre stuck for inspiration, look outside. In Australia, many residents have access to produce growing in the backyard, or community gardens and planter boxes.

I have a lemon tree and a lime tree and my neighbour has an orange tree, says Linklater. He says fruit can make a tasty addition to a drink, but citrus leaves and herbs are also aromatic add-ons. There are fig trees in my neighbourhood, so fig leaves are amazing, he says. If you can just pick a couple of herbs thyme or rosemary, mint, a little of coriander. Pepperberry leaf or the berry work really well.

If youre going to use produce, just make sure its fresh and delicious, he advises. You can always mix up a classic cocktail with your favourite piece of produce. Theres really nothing wrong with chucking strawberry in a Negroni, for example.

We asked Linklater for his batched Martini recipe.

Batched MartiniMakes 5 serves. Approx. 2.2 standard drinks per serve.

Ingredients:300ml Tanqueray London Dry Gin75ml dry vermouth125ml water1tsp white sugarGood pinch of salt

Method:Batch all ingredients into a clean bottle. Give it a good shake and keep in fridge. Half an hour before serving, place in freezer with your serving glasses. Garnish with an olive.

This article is produced by Broadsheet in partnership with Tanqueray London Dry Gin.

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The Ideal Anatomy of a Well-Stocked Liquor Cabinet - Broadsheet

Anatomy

The Anatomy of an Essay: Why Every Element Counts – http://www.newsgram.com/

Canada has a highly diverse population and features a wide range of industries, world-class metropolitan cities, and most importantly internationally accredited business schools. Students can pursue a masters in business administration with a specialized degree in finance, consulting or marketing. There are a lot of career opportunities in Canada, students can find work anywhere from the countrys financial services to any other sector. Many international students who study in the country receive their work visa just after completing their studies and can even expect to get a permanent residency after completing their studies. There are numerous benefits of doing MBA in Canada such as:

Quality education:Canada has a globally recognized world-class education system and is considered one of the best places to pursue MBA. Each year Canada attracts thousands of students from abroad. Its high academic standards and rigorous quality controls reflect the high-quality education provided which helps in successful and long term career plans.

Low tuition cost: Canada is often preferred choice for students because of the low tuition costs. Many organizations provide benefits to the students to pursue their studies successfully. The tuition fee that you pay may vary depending on where you choose to study. It is a developed country with a high-income economy and a successful government which makes it a fantastic place to live and study.

Multicultural experience: Universities come up with different events so that the students canmingle with multicultural people on and off the campus. Even though it is a mixed society but the universities and the government as well are trying to make sure that there wont be any problems of race and color. They encourage multi-cultural integration and Canada has a national policy for multiculturalism and works to ensure that peoples customs and traditions are preserved and respected.

Research:The study programs delivered by Canadian universities are more focused on research where students get to engage in interesting experiments and projects which are meant to provide students with an innovative study experience.

Internship programs: There are plentiful opportunities for business students who can combine academics with an internship or work experience in the field of your study which in turn can diversify and develop your professional skills.

Multilingual:English and Frenchare the official languages of Canada and it has become a multilingual country with the arrival of so many people from different nationalities with so many languages, religions, and cultures. It is a great opportunity for the students to learn different languages. Students can enrich their linguistic and communicative skills while being enrolled in a Canadian university.

Immigration: According to the national statistics office, the number of migrants entering Canada has grown from 250,000 last year to around 300,000 this year.On account of the open door policy of Canada, immigrants and their descendants have made immeasurable contributions to Canadas economy. After completing their studies, students with Canadian credentials and Canadian work experience can apply for permanent residency.

Beautiful landscape: Canada has spectacular scenery, landscapes that can be explored throughout the year. The cold season provides a great opportunity for dedicated skiers and ice skaters to practice their sport in majestic surroundings.

Canada has a highly diverse population and features a wide range of industries, world-class metropolitan cities, and most importantly internationally accredited business schools. Students can pursue a masters in business administration with a specialized degree in finance, consulting or marketing. There are a lot of career opportunities in Canada, students can find work anywhere from the countrys financial services to any other sector. Many international students who study in the country receive their work visa just after completing their studies and can even expect to get a permanent residency after completing their studies. There are numerous benefits of doing MBA in Canada such as:

Quality education:Canada has a globally recognized world-class education system and is considered one of the best places to pursue MBA. Each year Canada attracts thousands of students from abroad. Its high academic standards and rigorous quality controls reflect the high-quality education provided which helps in successful and long term career plans.

Low tuition cost: Canada is often preferred choice for students because of the low tuition costs. Many organizations provide benefits to the students to pursue their studies successfully. The tuition fee that you pay may vary depending on where you choose to study. It is a developed country with a high-income economy and a successful government which makes it a fantastic place to live and study.

Multicultural experience: Universities come up with different events so that the students canmingle with multicultural people on and off the campus. Even though it is a mixed society but the universities and the government as well are trying to make sure that there wont be any problems of race and color. They encourage multi-cultural integration and Canada has a national policy for multiculturalism and works to ensure that peoples customs and traditions are preserved and respected.

Research:The study programs delivered by Canadian universities are more focused on research where students get to engage in interesting experiments and projects which are meant to provide students with an innovative study experience.

Internship programs: There are plentiful opportunities for business students who can combine academics with an internship or work experience in the field of your study which in turn can diversify and develop your professional skills.

Multilingual:English and Frenchare the official languages of Canada and it has become a multilingual country with the arrival of so many people from different nationalities with so many languages, religions, and cultures. It is a great opportunity for the students to learn different languages. Students can enrich their linguistic and communicative skills while being enrolled in a Canadian university.

Immigration: According to the national statistics office, the number of migrants entering Canada has grown from 250,000 last year to around 300,000 this year.On account of the open door policy of Canada, immigrants and their descendants have made immeasurable contributions to Canadas economy. After completing their studies, students with Canadian credentials and Canadian work experience can apply for permanent residency.

ALSO READ:Credihealth Launches Dedicated Tele/Video Consultation Service

Beautiful landscape: Canada has spectacular scenery, landscapes that can be explored throughout the year. The cold season provides a great opportunity for dedicated skiers and ice skaters to practice their sport in majestic surroundings.

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The Anatomy of an Essay: Why Every Element Counts - http://www.newsgram.com/

Anatomy

Anatomy of a Hat-Trick: Lucas Moura Nets Stunning Treble to Send Tottenham to Champions League Final – 90min

After knocking out Real Madrid and Juventus in hugely impressive fashion, a youthful Ajax side were drawn against Tottenham Hotspur in the semi-finals of the Champions League in 2018/19.

The Dutch side's fairytale run looked set to continue as well, as they showed their maturity and class to pick up a 1-0 first leg win away from home. And they extended their lead when Matthijs de Ligt climbed highest after just five minutes in the second leg to power home and put Ajax in control.

Spurs weren't going down without a fight, however, and Son Heung-min rattled the woodwork. But it was Ajax who grabbed the next goal through Hakim Ziyech, the Moroccan firing in a stunning finish with his left foot to put his side very much in the driving seat and on course for a place in the final of the Champions League.

As the goal went in, Tottenham looked deflated, whereas Ajax were thriving and only growing into the contest. Normally, a 3-0 lead with 55 minutes left to play would be too high a mountain to climb for a team in the Champions League semi-finals, but this wasn't any ordinary contest.

Having witnessed one of the most remarkable comebacks in recent history the night prior as Liverpool overturned a 3-0 first leg deficit to beat Barcelona, Tottenham - and the footballing world - knew that anything was still possible.

On the biggest stage, with so much at stake, Spurs required their star players - or a star player - to take matters into their own hands and defy the odds.

Lucas Moura was this man on 8 May 2019.

His stunning hat-track sparked wild celebrations in the away end of Johan Cruijff Arena, but left those in the other parts of the stadium shellshocked and heartbroken.

For the second time in two nights, the footballing world had been treated to an unthinkable comeback. So let's do Lucas' hat-trick justice by breaking it down and looking at each of his strikes on the night individually....

The timing of the first goal was crucial, coming just ten minutes after the restart. While Lucas was the one to receive all the credit for pulling a goal back, it was actually a pulsating and devastating counter-attack.

Starting in defence, Danny Rose produced a deft little nutmeg to get past De Ligt, before pinging the ball in the direction of Lucas, who touched it first-time into the path of Dele Alli.

Dele only had one thought in his mind - drive at the defence. Running towards goal, he chopped inside the defender, and Lucas used his pace to ensure he would be the first man to latch onto the loose ball. The Brazilian took just one perfect touch away from the defenders before ruthlessly firing his left-footed strike into the bottom corner.

No celebration, instead the players raced to collect the ball and place it back in the centre circle.

Game on.

It was a stunning counter-attack that often (understandably) gets overlooked due to the overall events on the night.

If the first goal was a thing of beauty, the second was...well, it was more about sheer perseverance more than anything.

Just four minutes later, Rose's cross from the left found its way to Son on the edge of the area. The forward saw Kieran Trippier bursting into the box on the right and played the ball in front of him, allowing the English full-back to burst onto it at full pace. As Fernando Llorente met Trippier's drilled low cross, it appeared as though it was a certain goal.

But Andre Onana had other ideas.

It was a breathtaking save from point-blank range, but as he rushed to pick up the loose ball, he was blocked by his own defender. In came Lucas, racing onto the ball, but with nine (NINE!) Ajax players all stood within 12 yards of the goal as they looked to preserve their lead, it looked as though the 27-year-old would struggle to find any real space to get a shot in.

Twisting, turning and shifting with the ball stuck to his feet, Lucas managed to find less than half a yard of space before unleashing his strike, which found its way into the bottom corner.

While this goal was about perseverance, the footwork from Lucas to find the space he needed is something to be admired.

Two goals in four minutes from Tottenham, but this was an Ajax side that were fearless, and they looked a threat each time they went forward.

Indeed, it was Ziyech again who came closest to extending their lead on aggregate, the player drilling his shot against the post.

It was a lucky escape for the north Londoners. But then it was their turn to be denied by the woodwork, this time Jan Vertonghen saw his header hit the bar before being cleared off the line.

The game was on a knife-edge.

The clock entered into added time, with Spurs still searching for the winner - the goal to take them to the Champions League final. Each minute for them flew by, and with 96 minutes now having been played, Moussa Sissoko realised that the ball had to go long.

Llorente used his body well to stop an Ajax player from getting a head to it and clearing, instead it fell into the path of Dele. The midfielder played the ball first-time through to Lucas, who made a darting run from the right into the centre of pitch.

Instinctively, he fired the ball low and hard into the bottom corner, with Onana stretching but unable to keep it out.

The Ajax players were on the floor, they couldn't believe what had just happened. As for Spurs, it was a moment that will live long in the memory of those involved with the club.

As the full time whistle blew, Mauricio Pochettino was in tears, while Harry Kane had seemingly recovered from an ankle injury for those few moments as he was sprinting around the pitch. Lucas, meanwhile, the hero of the night, was on the floor in shock before teammates circled around him and lifted him up to celebrate.

Lucas' ruthless finish in the dying seconds of the game summed up his overall performance on the night, stepping up and delivering for his team to make it one of the most memorable nights in the club's recent history.

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Anatomy of a Hat-Trick: Lucas Moura Nets Stunning Treble to Send Tottenham to Champions League Final - 90min