Category Archives: Biochemistry

This is one place on Earth where no life can exist – WBAP News/Talk

Life exists in extreme environments on Earth, from arid deserts and frozen tundras to thermal, toxic vents in the deepest reaches of the ocean floor. But it cant exist on every inch of the planet and scientists have discovered a place in Ethiopia where life cant find a way, according to a new study.

In contrast with previous research, scientists conducted multiple tests and found that there is no life, not even microorganisms, in Dallol. One of Earths most extreme environments, Dallol is incredibly hot, salty and acidic. Its ponds extend across a volcanic crater, in the Ethiopian Danakil depression, filled with salt, toxic gases and boiling water in response to extreme hydrothermal activity.

Even in winter, daytime temperatures can exceed 113 degrees Fahrenheit. Some of the hyper acidic and saline pools have negative pH values.

The findings published Friday in the journal Nature Ecology & Evolution.

After analysing many more samples than in previous works, with adequate controls so as not to contaminate them and a well-calibrated methodology, we have verified that theres no microbial life in these salty, hot and hyperacid pools or in the adjacent magnesium-rich brine lakes, said Purificacin Lpez Garca, study author and biologist at the French National Centre for Scientific Research.

However, outside of the ponds, its a different story.

What does exist is a great diversity of halophilic archaea (a type of primitive salt-loving microorganisms) in the desert and the saline canyons around the hydrothermal site, but neither in the hyperacid and hypersaline pools themselves, nor in the so-called Black and Yellow lakes of Dallol, where magnesium abounds, said Lpez Garca. And all this despite the fact that microbial dispersion in this area, due to the wind and to human visitors, is intense.

The researchers performed mass sequencing of genetic markers meant to find and classify any microorganisms that may be present, as well as cultures to find microbes, cytometry for detecting individual cells, brine chemical analysis and electron microscopy combined with X-ray spectroscopy.

At first glance, minerals rich in silica may mimic microbial cells, the researchers said. But their analysis revealed the difference.

In other studies, apart from the possible contamination of samples with archaea from adjacent lands, these mineral particles may have been interpreted as fossilized cells, when in reality they form spontaneously in the brines, even though there is no life, Lpez Garca said.

Scientists have used evidence of life in extreme environments on Earth as an analog for the conditions where life may exist on other planets in our solar system or beyond it. The researchers warned that in this case, just because there is liquid water present or because something resembles cells or other biological aspects beneath a microscope, does not mean there is life present.

Our study presents evidence that there are places on the Earths surface, such as the Dallol pools, which are sterile even though they contain liquid water, Lpez Garca said.

The Dallol ponds actually prevent life from forming because they contain chemical barriers like chaotropic magnesium salts that help break down hydrogen. Combined with the salty, acidic and hot environment, life receives no encouragement in the pools.

We would not expect to find life forms in similar environments on other planets, at least not based on a biochemistry similar to terrestrial biochemistry, said Lpez Garca.

The researchers will continue studying the pools to determine more about the limits of life.

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This is one place on Earth where no life can exist - WBAP News/Talk

Global Automated Biochemistry Analyzers Market 2019 by Manufacturers, Regions, Type and Application, Forecast to 2025 – The Industry Press Releases

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Global Automated Biochemistry Analyzers Market 2019 by Manufacturers, Regions, Type and Application, Forecast to 2025 - The Industry Press Releases

Scientists unravel mysteries of cells’ whiplike extensions | The Source – Washington University in St. Louis Newsroom

Cilia, or flagella whiplike appendages on cells perform diverse tasks required to keep the body healthy. When cilia malfunction, the consequences can be devastating, causing a range of problems, from blindness, to lung and kidney diseases, to congenital heart defects. Now, scientists have revealed the firstdetailed lookat the inner structure of cilia.

The newly revealed structure offers a starting point to begin exploring how cilia are assembled during development, how they are maintained over a cells life span, and how they might become dysfunctional if some of the cogs in these complex molecular machines are mutated or missing. The structure of these microscopic molecular machines common to cells in organisms from algae to people potentially will answer questions about human health and disease.

The research, by investigators at Washington University School of Medicine in St. Louis and Harvard Medical School, was published recently in the journal Cell.

This new study is exciting because it fills in a lot of missing information about the structure of cilia, said senior authorRui Zhang, assistant professor of biochemistry and molecular biophysics at Washington University. When cilia dont work properly, bad things happen. We need to know details of the structure in order to develop treatments for diseases, or strategies to prevent the developmental defects that can occur in the early embryo if the cilia are not functioning as they should.

In the respiratory tract, cilia move mucus and protect against viral and bacterial illnesses. In the reproductive tract, they propel sperm to fertilize an egg. Cilia also perform vital tasks in the brain, the kidney, the pancreas and in bone growth. And in the earliest stages of development, the rotational motion of specialized cilia in the embryo defines the bodys left-right asymmetry and where organs are placed. Without properly functioning cilia, the heart may not end up on the left side, where it should be, and it may not function properly.

Cilia are implicated in multiple human disorders, including polycystic kidney disease, which affects some 600,000 Americans and requires dialysis; primary ciliary dyskinesia, which causes chronic lung disease, misplaced organs and infertility; Bardet-Biedl syndrome, which causes patients to become blind in childhood and leads to diabetes, kidney disease and extreme obesity; and many congenital heart defects, which occur when left-right asymmetry goes awry and require complex surgeries to repair.

In the new study, the researchers used a technique called single particle cryo-electron microscopy to get a first look at 33 specific proteins arranged inside cilia within structures called ciliary microtubule doublets in a strict repeating pattern.

Before this work, everyone assumed these proteins inside cilia just stabilize the structure, which is true for a subset of the proteins, especially when you consider the forces produced by the continuous beating of the cilia, Zhang said. But based on how they are arranged inside this structure, we believe these proteins are doing many more things.

Since many of the proteins protrude through the cilia, Zhang and his colleagues speculate that they may allow for communication between the inside and the outside of the ciliary microtubule doublets; govern the function of enzymes that make important biochemical reactions possible; and sense changes in the calcium concentration of the environment, which plays a role in triggering the cilia to beat.

Among the proteins identified, five are associated with diseases that have been studied in mice and people, said co-authorSusan K. Dutcher, professor of genetics at Washington University. But until now, no one knew that these proteins were found inside cilia. We are just beginning to understand their roles in normal and disease states.

The researchers studied cilia in a type of algae calledChlamydomonas reinhardtii, which are single-celled organisms that have cilia structurally and biochemically similar to those of more complex organisms, including people. One question Dutcher is interested in answering is how the proteins making up cilia structure govern the type of motion that the cilia perform. The cilia of single-celledC. reinhardtiiare capable of more than one type of motion.

In some situations, the cilia are doing what you might consider a breast stroke, Dutcher said. In others, the motion is more of an S-shaped wave. The cilia of many cells in mammals can only produce one of these motions. But the single-celledC. reinhardtii, perhaps to help it adapt to its environment, can switch between them. Thats why were studying algae at a medical school the genetic problems we can study in the cilia of these organisms are similar to the ones that can occur in people, often with devastating consequences.

Zhang, Dutcher and their colleagues have plans to use the latest techniques of cryo-electron microscopy to study theChlamydomonas mutants of each of the 33 proteins inside cilia to seek answers to many questions that have arisen from this new and detailed knowledge of the structure.

Originally published by the School of Medicine

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Scientists unravel mysteries of cells' whiplike extensions | The Source - Washington University in St. Louis Newsroom

Semester in the sun – Gazette

Part of a special feature highlighting the student voice, student experience and the range of student supports and opportunities available at Memorial.

Nov. 22, 2019

Amelia Lacey will likely spend some time studying on an Aloha State beach during the winter 2020 semester.

The fourth-year biochemistry (nutrition) major and St. Johns resident will study at the University of Hawaii at Mnoa as one of 20 top Canadian students receiving a Killam Fellowship from Fulbright Canada this year.

The scholarship program allows exceptional undergraduate students to participate in a bi-national academic exchange between Canada and the United States.

I wont be able to come home for a visit while Im there, and Ive never lived away from home for that long before, so its definitely going to be a new experience for me, said Ms. Lacey about the upcoming experience.

But, Im looking forward to all of the adventurous activities there and I hope to explore the islands of Hawaii as much as I can.

Ms. Lacey says her success in receiving the fellowship is an example of what can happen when you dont give up. She applied for the Killam Fellowship twice before being successful this year let alone in the highly coveted Paradise of the Pacific spot.

You have to choose the university that best matches the courses you need to take for your degree, and Hawaii has a great nutrition program, said Ms. Lacey. I recognized that and highlighted it in my application. They also offer a lot of courses we dont have at Memorial, so Im hoping to take a few courses I wouldnt otherwise get to take.

Photo: Submitted

In September she travelled to Ottawa, Ont., for an orientation session. There, she met the other Canadian and American Killam fellows, along with the American Fulbright students and scholars who are studying and conducting research in Canada this year. This spring Ms. Lacey will gather with them once again for a seminar in Washington, D.C.

It was great to meet people who were very different from me, but who also had similar values, she continued. I only spent three days with them, but were still talking regularly. I know well be keeping in touch.

It showed her, as many past Memorial fellowship recipients have found, that the Killam network is a strong one.

Once you get a Killam, youre considered a Killam fellow for life. So, you will continue to get emails, event invitations and other opportunities from them. It certainly opens up doors, thats for sure.

A focus on health and well-being has guided Ms. Laceys academic and personal pursuits.

She has been awarded several research awards, including three MUCEPs, a Natural Sciences and Engineering Research Council of Canada Undergraduate Student Research Award and a Faculty of Medicine Summer Undergraduate Research Award.

A Deans List student for the past three years, she has also been supported by several generous scholarships. Currently, she is the vice-president (social) for the Biochemistry Society, executive director of communications with the Women in Science and Engineering Undergraduate Society and a member of the Quintessential Vocal Ensemble. Past volunteer work with Global Brigades in Honduras also inspired Ms. Lacey to become more involved in global health.

Kelly Foss is a communications advisor with the Faculty of Science. She can be reached at kfoss@mun.ca.

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Semester in the sun - Gazette

Second-year swimmer excited for new season – The Observer

Kate Ptashnik is a second-year distance free swimmer on the Case Western Reserve University swim team. With the season still young, she looks to improve on a stellar first year that featured an 18:21.88 in the 1,650 free. Swimming provides Ptashnik with countless memories, including getting punched in the face. Ptashnik is currently majoring in nutritional biochemistry and likes to spend time with her friends.

What is your favorite part of being on the swim team? Why?

My favorite part of being on the swim team is being surrounded by people who have similar work ethics, but different passions than I do. I enjoy hearing and seeing my teammates/friends being excited about things they are doing while we get to bond through something we all love.

Do you have any meets that you remember being particularly special? Why?

The Wooster Invitational meet last year, I was accidentally punched in the face by another athlete right before my race (the mile), but then proceeded to swim my best time of the season.

Aside from swimming, what other activities are you involved in on campus? Can you explain what you do in them?

I am the Greek Games chair and the executive assistant in Kappa Alpha Theta. I am also a tour guide at the CWRU Office of Admissions. Additionally, I am a part of research within the nutrition department, where we are trying to teach children with family members who have diabetes how to make healthy and nutritious foods.

Why are you majoring in nutritional biochemistry? What about it interests you?

I am majoring in nutritional biochemistry because I liked both biology and chemistry, and then got early exposure to a nutrition course my first semester here and absolutely loved it. I am interested in nutritional biochemistry because I think it is really applicable to a student athlete as well as just a person.

What are your favorite free time activities? Why?

Spending time with my friends, both on and not on the swim team, because we can always have fun no matter what else is happening in our stressful CWRU life.

What is your favorite quote or what inspires you?

Be the hammer Doug Milliken.

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Second-year swimmer excited for new season - The Observer

Curcumin with piperine supports liver function, says recent study – Nutritional Outlook

A study recently published in the Journal of Cellular Biochemistry1 found that supplementation with Sabinsas patented curcumin extract Curcumin C3 Complex combined with the patented piperine BioPerine, supported liver function in subjects with Non-Alcoholic Fatty Liver Disease(NAFLD). A chronic liver condition that effects about 25% of the U.S. population, according to the American Liver Foundation, NAFLD has no approved pharmaceutical treatments, and is commonly associated with metabolic syndrome and cardiovascular diseases.

In the study, 70 subjects with ultrasound-determined NAFLD were randomized to either receive 500 mg of curcumin extract with 5 mg of piperine daily, or placebo for 12 weeks. Results showed that supplementation significantly reduced elevated liver function parameters including alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, as well as cholesterol, low-density lipoprotein cholesterol, iron, hemoglobin, and increased total iron-binding capacity. Based on sonography, the supplement group also saw improved NAFLD severity, compared to placebo.

We're encouraged by the researchers conclusion that combined supplementation of C3 Complex and BioPerine may provide a safe and viable method for curtailing the progress of NAFLD, said Sabinsa founder Muhammed Majeed, PhD, in a press release.

References:

1. Panahi Y et al. Curcuminoids plus piperine improve nonalcoholic fatty liver disease: A clinical trial. Journal of Cellular Biochemistry, vol. 120, no. 9 (2019): 15989-15996

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Curcumin with piperine supports liver function, says recent study - Nutritional Outlook

New Database Could Help Identify Therapeutic Targets for the Creation of Antibiotics – Technology Networks

Infectious diseases are caused by pathogenic microorganisms capable of entering, colonising and growing within a host organism, thus producing an infection. Bacterial infections have been on the rise worldwide in recent years, but many mechanisms underlying bacterial pathogenesis are still poorly understood. This is highly relevant given the fact that the development of new antimicrobial therapies is largely based on current knowledge of the mechanisms behind these infections. The proteins coded by the bacterial genes are responsible for the thousands of biochemical processes essential for the efficient propagation of the pathogen. Many studies demonstrate, however, that in order to identify these genes, in vivo information is needed on what happens with the bacteria in a real case of an infected host. The in vitro studies, i.e., those recreated in laboratories with cell and bacterial cultures, later do not always correlate with data from in vivo studies. This is due to the fact that pathogenic bacterial genes essential for producing the infections depend on the environment of the colonised organism.A team of researchers from the Department of Biochemistry and Molecular Biology of the Universitat Autnoma de Barcelona and from the Centre of Genomic Regulation (CRG) has created the BacFITBase database. Based on the results of the in vivo experiments, researchers systematically characterised the bacterial genes relevant for host cell invasion and infection. All the experiments performed were based on a technique named transposon mutagenesis, where the DNA fragments called transposons are transferred to the organism's pathogenic genes, thereby inactivating them. By doing so, their role in the infection can be observed directly and researchers can determine which are essential for a specific host organism to become infected. Therefore, this database will make it easier to identify target proteins that can help in fighting infectious diseases and accelerate the development of new antimicrobial agents.The database contains over 90,000 entries with information on specific pathogenic bacterial genes and their contribution to in vivo infectious conditions in five different host species. It includes information on a total of 15 bacteria (two variants ofSalmonella enterica, Haemophilus influenzae, Streptococcus pyogenes, Porphyromonas gingivalis, Mycobacterium avium,three variants ofEscherichia coli, Vibrio cholerae, Campylobacter jejuni, Klebsiella pneumoniae, Acinetobacter baumannii, Serratia marcescensandVibrio parahaemolyticus) and 5 model vertebrates (cow, pig, hen, mouse and rabbit), with information across 10 different tissues.BacFITBase, published in the journal Nucleic Acids Research, was developed by researchers from the UAB Department of Biochemistry and Molecular Biology Javier Macho and Marc Torrent, alongside researchers from the Centre for Genomic Regulation (CRG) Benjamin Lang and Gian Gaetano Tartaglia.

Reference:Rendn,et al. (2019) BacFITBase: a database to assess the relevance of bacterial genes during host infection.Nucleic Acids ResearchDOI: https://doi.org/10.1093/nar/gkz931

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.

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New Database Could Help Identify Therapeutic Targets for the Creation of Antibiotics - Technology Networks

Battle of the Bands winners mix hard work with passion – Daily Trojan Online

Lori Loughlins Lonely Hearts Club Band, featuring musicians JP Dabu, Niles Miller, Joe Aleshaiker and Bruno Pita (right to left), beat out the competiton at Ground Zeros annual Battle of the Bands. (Photo courtesy of JP Dabu)

What do you get when a biochemistry major, a journalism major, a business major and a civil engineering graduate start a band? It sounds like the beginning of a bad joke, but the four members of Lori Loughlins Lonely Hearts Club Band have proven otherwise. On Oct. 25, they competed against three other groups at USCs Battle of the Bands and won a $300 Amazon gift card as a testimony to their talent.

Lead vocalist and keyboardist Joseph Dabu, or JP, was scrolling through his Instagram feed when he saw the application for the contest. He immediately contacted his high school friend Bruno Pita, a biochemistry graduate student at UCLA who plays the bass. Pita then contacted fellow Bruin Joe Aleshaiker, a recent alumnus and civil engineering major.

Lastly, Dabu reached out to Niles Miller, a junior majoring in business of cinematic arts who he met at Remedy Through Music, a student organization dedicated to visiting local hospitals and retirement homes and playing songs for the patients, nurses and visitors.

Before they even started playing, what first characterized their personality was their groups name witty, original and just a little bit controversial.

We couldnt come up with a name. We had a placeholder name [The Rivals] which was terrible, Pita said. I was actually ashamed of going up on stage like that I couldnt think of anything that was acceptable, Pita said.

On the last day the band was able to change its name for the competition, Pita desperately pulled up the Los Angeles Times and saw a headline about Lori Loughlins indictment. A lightbulb went off in his head. The alliteration of the three Ls was brilliant, and the callback to the quintessential Beatles album worked perfectly.

Its funny because both Bruno and Joe are from UCLA and Niles and I are from USC, Dabu said.

But Pita hopes no ones feelings were hurt.

I like to think of it as making your food spicy, Pita said. It might sting a bit but it makes the food more interesting to eat.

Although the spice in their name is certainly ear-catching, the food or in this case, their music is what kept the audience enthralled and eventually led them to victory. Their renditions of Dont Let Me Down, Something and Somebody to Love showcased their skills as musicians and proved that you dont have to major in music to find your passion in music.

Aleshaiker is living proof of this. Since graduating, he has been working on his music full time.

Ive been playing since I was 9 years old, so its always been a passion of mine, he said. For me at least, its definitely a big part of my life.

Likewise, Miller, the bands drummer, said his passion for music has always been present in his life. He learned to play the djembe and bongos with his dad and went on to play percussion and drums in middle school and high school.

I did do jazz ensemble for about a year, but then I stopped because it was too much with my current workload, Miller said. However, he still considers playing the drums a great passion.

Pita, on the other hand, only started playing music his junior year, learning to play the piano on an iPad app and eventually picking up the guitar. But unlike his bandmates, Pita mostly sees the instrument as a stress-relieving hobby.

Thats how we actually started jamming together, he was just so hyped I think to have someone that played with him, Pita said.

Even though Lori Loughlins Lonely Hearts dont identify as a Beatles cover band, the undertone and general respect for the iconic group is clear in their bands style, and each member identifies with an individual Beatle.

Im George, 100%, Aleshaiker said. I feel like George is always kind of in the background but is always contributing.

The other three have yet to reach some consensus. Bruno says hes a Paul because he plays the bass and sings the higher harmonies. Dabu says hes a Paul because hes left-handed. And although one might be tempted to call the drum player a Ringo, Miller objected.

I decided to leave it up to the BuzzFeed quiz on there, Miller said. And I got that Im a Paul!

Even though Lori Loughlins Lonely Hearts Club Band started out as a one-time performance, their win at Battle of the Bands was a definite confidence booster.

There was a lot of good competition, so I was actually kind of surprised that we won, Aleshaiker said.

Dabu agreed and lauded the other three bands they competed against.

We just banded together in September and the other bands Im sure, had been established, and probably were practicing more often than we were, he said.

But Natalie Williams, a freshman majoring in art who attended Battle of the Bands, dismisses their humble remarks.

I wasnt really surprised they won, she said. They had a lot of energy and were really hyped up. It was obvious they practiced a lot.

In the end, it wasnt just their hard work but their passion for music indisputably paid off.

We just wanted to have a good time, Dabu said. Im glad we did that.

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Battle of the Bands winners mix hard work with passion - Daily Trojan Online

UNL students give their thoughts on impeachment hearings – Daily Nebraskan

Impeachment hearings are underway on Capitol Hill as the U.S. House of Representatives Permanent Select Committee on Intelligence works to decide if there will be an impeachment trial for President Donald Trump. The Daily Nebraskan asked students if they have been paying attention to the hearings and if they think impeachment is likely.

Pierce Leef, sophomore biochemistry and science education double major

Pierce Leef, sophomore biochemistry and science education double major

I havent been really paying attention. Ive wanted to, but theres a lot of other stuff going on right now, and its really busy. I personally hope that there is something involved with at least the process of going through and seeing what actually has happened and what hasnt because theres been a lot of misinformation on both sides. Just figuring out the real truth would be good.

Cleopatra Babor, freshman plant biology major

Cleopatra Babor, freshman plant biology major

I have been paying attention, and I believe that impeachment is very unlikely. Just all the information [President Trump] has been hiding is probably the most shocking thing, but in my personal opinion, we kind of all already knew [he was hiding information]. Its very close to the 2020 election and thats why I think impeachment is pushing to the unlikely side. But theres still hope.

Louis Lu, junior actuarial science major

Louis Lu, junior actuarial science

I havent been paying attention. For me, I am not really sure about politics in America because I dont really care about that because Im a Chinese student. Maybe [impeachment] is possible.

Alex Bartels, junior advertising and public relations major

Alex Bartels, junior advertising and public relations major

I have not been paying attention to the impeachment hearings. I just dont really pay attention to politics and dont really follow anyone on social media that talks about politics. I dont know much about it and also just dont really know if [impeachment] is possible or not. I feel like they probably wont impeach since the election is already coming up next year.

Ali Mohamed, junior education major

Ali Mohamed, junior education major

Ive seen clips here and there, but I havent been really paying attention. [Impeachment] is probably not going to happen. Its been going on for a while, and I feel like theyre dragging it on. By that time, hes probably going to be out of the office.

news@dailynebraskan.com

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UNL students give their thoughts on impeachment hearings - Daily Nebraskan

Longhorn Stop the Bleed receives 5 years of funding to expand emergency response training – UT The Daily Texan

The previously unfunded Longhorn Stop the Bleed program recently received five years of funding from Campus Safety and Security.

The program is a chapter of a national organization created after Sandy Hook to teach people how to respond in bleeding emergencies. UT alumna Claire Zagorski said she started a chapter of the program on campus to reduce fatality and helplessness.

I was inspired to bring it to UT after Harrison Brown was stabbed to death, program director Zagorski said. The bystander was saying, This is terrible because he was laying there bleeding and no one could do anything, just wait. Im a paramedic, and I knew that wasnt true, but I also knew people had to be taught what to do.

Zagorski said the funding will consist of $2,500 every year and is the result of safety advocacy nonprofit SafeHorns stressing the programs importance to Campus Safety and Security.

It was very validating, Zagorski said. Im glad that the work that were doing is getting noticed and that were making a difference, and now were going to be able to take a big step forward in expanding the program because of that.

Zagorski said the program will use the funds to buy more training equipment for larger classes and to launch a promotional campaign to increase awareness. She said the training is useful in many contexts, but people pay attention to the program if they see a recent uptick in campus violence.

UT is an open campus, biochemistry sophomore Sonia Patel said. I think its a necessity for people, and students especially, to be aware of what to do in the event of a shooting or stabbing. If we were aware of it, we could help save people.

Zagorski said the program partners with the certified EMTs in Longhorn EMS to lead training courses. David Wu, lead instructor of Longhorn Stop the Bleed in Longhorn EMS, said empowerment is a huge goal of the training.

It isnt necessarily having the skills, biochemistry junior Wu said. Its having the confidence of knowing you have the training to know what to do in that scenario. Most bystanders are in shock when something happens, and with a little training, it gives you a bit of confidence to go, Okay, I know what to do in this situation. This is how I should move forward.

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Longhorn Stop the Bleed receives 5 years of funding to expand emergency response training - UT The Daily Texan