Category Archives: Anatomy

Meet the 7 Pinoy virologists who will be at heart of anatomy of soon-to-rise PH Virology and Vaccine Institute – Manila Bulletin

Balik Scientists. Balik Puso. Balik Pilipinas.

Seven Filipino virologists who were trained abroad responded to the call of the Department of Science and Technology (DOST) to be part of the first-of-its-kind Virology and Vaccine Institute of the Philippines or VIP.

As they are scheduled to pay a courtesy call on Department of Science and Technology (DOST) Secretary Fortunato Boy T. de la Pea on Friday, Aug. 13, they are expected to share their expertise in the study and development of local vaccines against the coronavirus disease (COVID-19) and their scientific researches on virology and other diseases.

It has been noted that from 1975 to 2020, the DOST was able to work with 564 Balik Scientists through 716 engagements under its brain gain flagship initiative, Balik Scientist Program (BSP).

The Balik Scientist Program has been bringing home Filipino scientists from all corners of the world for almost 46 years now, DOST Undersecretary for Research and Development Rowena Cristina L. Guevara told the Manila Bulletin.

As the Philippines continues to fight and brace itself for the effects of the pandemic, we saw the eagerness and dedication of our Balik Scientists to help our country. We wish to express our gratitude to our Balik Scientists for their unconditional and unwavering love and service to the country.

As the DOST is set to roll down its red carpet to the seven Balik Scientists, it is good to know their affiliations and expertise, putting them at the heart of the anatomy of the VIP which may rise at the New Clark Economic Zone in Capas, Tarlac by the end of 2023 or in 2024.

The profiles of the seven Filipino virologists were provided by Office of the Undersecretary for Research and Development, led by Guevara.

DR. ELPIDIO CESAR NADALA JR.

Ph.D. in Microbiology and Animal Virology

Post Doctoral in Aquatic Virology and Medical Biotechnology

-Vice President of Research and Development, Diagnostics for the Real World, Ltd (DRW), California, USA

Dr. Elpidio Cesar Nadala Jr. is a virologist and microbiologist with 20 years of experience in academic research and 17 years in the industry developing diagnostic assays for the detection of bacterial and viral pathogens.

He is the co-founder of the Diagnostics for the Real World, Ltd (DRW), where he spearheaded the development of rapid diagnostic tests for the detection of Hepatitis B Virus (HBV) surface antigen, Human Immunodeficiency Virus (HIV) antibodies, and Hepatitis C Virus (HCV) antibodies, as well as improved versions of the CE-marked Chlamydia rapid test.

His team was behind the development of the SAMBA II SARS-CoV-2 test for detection of SARS-CoV-2 RNA. The SAMBA II SARS-CoV-2 test was developed and validated within two months in 2020.

Currently, the SAMBA II SARS-CoV-2 test is used in 79 hospitals and schools in the United Kingdom with a total of 648 Assay Modules deployed and 300,000 tests used so far.

DR. LOURDES NADALA

Ph.D. in Microbiology (Major in Animal Virology)

-Vice President, Regulatory Affairs/Quality Assurance, Diagnostics for the Real World, Ltd (DRW)

Dr. Lourdes Nadala is a microbiologist/virologist by training with over 15 years of experience in microbial systematics and culture collection-related activities and over 20 years of shrimp and human diagnostics, including ISO accreditation and successful regulatory submission of in vitro diagnostic medical devices.

She spearheaded DRWs efforts for ISO 13485 accreditation and regulatory approval of in vitro diagnostic (IVD) products which include molecular point-of-care tests for early diagnosis of HIV in infants, viral load monitoring, and recently, SARS-CoV-2 Test using the SAMBA platform.

She also has extensive experience in field evaluations and clinical trials in IVDs.

She also worked on detection methods for infectious pathogens and spoilage organisms affecting food safety, ISO accreditation for 9001 and ISO Guide 34 (Reference materials), AOAC validations, and customer-driven research projects related to food safety and shelf life.

DR. TEODORO FAJARDO JR.

Ph.D. in Molecular Biology (Major in Molecular Biology and Molecular Virology

Post Doctoral Fellow at London School of Hygiene and Tropical Medicine, UK with PhD in Public Administration (Major in Public Management)

-Healthcare Scientist Team Manager , UK National External Quality Assessment Service (NEQAS)

Dr. Teodoro Fajardo Jr. has specialization in Molecular Virology and Molecular Biology (which includes but not limited to: gene cloning, virus isolation, culture, propagation, and virus plaque assay, TCID50, reverse genetics virus propagation, generating infectious RNA of (+) RNA virus creating virus progeny from in-vitro transcribed RNA, and other molecular virology techniques (in vitro RNA synthesis of full-length viral RNA, in vitro RNA transcription, cell-free and mammalian cell mRNA translation, cell-free viral assembly system, RNA-RNA interactions assay for viral RNA packaging and assembly, nucleic acid isolation and purification, site-directed mutagenesis and cloning,gel electrophoresis, viral protein isolation and analysis, western blot, in vitro and in vivo gene expression of monocistronic and bicistronic mRNA, in-vitro mRNA translation, transfection of cultured cell, viral plaque analysis, analysis of expressed viral proteins)

DR. MYRA HOSMILLO

Ph.D. in Molecular Medical Science

Post Doctoral Fellow in Professor Ian Goodfellows Lab, Division of Virology, Department of Virology

-Research Associate, Division of Virology, Department of Pathology, University of Cambridge

Dr. Myra Hosmillo is an expert in the field of virology, specifically on veterinary and medical virology with a 12-year research career largely focused on virus-causing gastroenteritis.

She first worked on animal viral gastroenteritis caused by rotaviruses, caliciviruses, hepatitis E virus, toroviruses, and coronaviruses.

She was also involved in developing experimental systems to use animal viruses as a surrogate model of human viruses; particularly established an enhanced cell culture system and reverse genetics of porcine sapovirus to study its virus replication and the interactions with host innate immune response.

In Cambridge, she developed fundamental systems to directly study the pathogenesis of human norovirus using human intestinal organoids and replicon systems in human gastric tumor cells.

DR. CHRISTINA LORA LEYSON

PhD in Infectious Diseases

-Postdoctoral Researcher, Exotic and Emerging Avian Viral Diseases Group, Southeast Poultry Research Laboratory (SEPRL)

Dr. Christina Lora Leyson is an expert in standard DNA, RNA, and protein laboratory techniques including quantitative PCR, cloning, and protein expression and knowledge on sequence analysis, phylogenetics, and homology modeling with basic coding skills using Python for applications such as data wrangling and analysis.

DR. HOMER PANTUA, DVM

Ph.D. in Biomedical Sciences specializing in Immunology and Virology

-Genentech, South San Francisco, CA, USA; BioAssets Corporation

Dr. Homer Pantua is an Innovative and motivated infectious disease drug discovery scientist with over 13 years of industry experience as the lead scientist.

He has strong technical skills that encompass microbiology, molecular biology, immunology, biochemistry, and biotechnology.

He has an excellent track record of working as a lead scientist in diverse projects that progressed from early discovery to development.

DR. LEODEVICO L. ILAG

Ph.D. in Microbiology and Immunology; Postdoctoral Research Fellow in Structural biology of viruses and viral proteins

-Molawin Creek Ventures Pty. Ltd. (Melbourne, Victoria, Australia); Xerion Ltd (Brighton, Victoria, Australia); Philippine Asian Biotechnology R&D, Inc. (Manila, Philippines); Plentex Philippines, Inc, Plentex Realty Inc, PlentexAgri-Milling Corp, Plentex Aquaculture Corp (Tacloban, Leyte, Philippines)

Dr. Leodevico L. Ilag is a veteran microbiologist and immunologist with 25 years of solid research in discovery, preclinical and clinical development of biologics and dietary/food ingredients (peptides, proteins, antibodies, natural products, agricultural waste streams) with applications in oncology, cardiovascular disease, CNS, infectious disease, metabolic disease, and dermatology.

He has technical experience in antibody engineering; structural biology; virology; immunology; biochemistry; microbiology; genetics.

He has 25 years of international (Europe, US, Asia, and Australia) entrepreneurial biotechnology experience (R&D, business development/licensing/deal-making, strategy, general management, intellectual property management, financing, and operations) from conception through seed financing to initial public offering (IPO) raising the equivalent of more than A$80 million.

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Meet the 7 Pinoy virologists who will be at heart of anatomy of soon-to-rise PH Virology and Vaccine Institute - Manila Bulletin

‘Anatomy of a Murder’ Dissected | Northern Today – Northern Today

Author and former Army Judge Advocate Eugene Milhizer will give a free presentation on his book, Dissecting Anatomy of a Murder, on Thursday, July 29. The event begins at 2 p.m. at the Central U.P. and Northern Michigan University Archives in 126 Harden Hall at NMU. A book signing will follow.

Milhizer carefully and intelligently unravels the trial, book and movie based on one of the most famous criminal trials of the 20th century. He explores the 1952 murder in Big Bay and community attitudes about the crime. He tells the fascinating story of Anatomy of a Murder author John Voelker, who served as defense counsel for the resulting trial and then adapted the real-life courtroom drama into fictional form as a great novel. He also looks back on production of the groundbreaking film inspired by Voelker's novel, directed by Otto Preminger.

Milhizer's book considers several discrete legal and ethical issues that the novel and film raise. These include the implications of a criminal attorney explaining the law to a client in a manner that may suggest a dubious defense. It also reflects upon broader questions, such as the proper role of a jury and the impact of community standards in a criminal trial. It evaluates the capacity of the criminal justice system to achieve true justice within the context of what Voelker called the settled procedures and ancient rules of the law. And ultimately, it chronicles what some have called the greatest legal war story ever told.

For three years, Milhizer held a teaching appointment at the Judge Advocate General's School at the University of Virginia. In 2001, he joined the Ave Maria School of Law faculty, where his course offerings have included criminal procedure, criminal law, national security law, and military law.

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'Anatomy of a Murder' Dissected | Northern Today - Northern Today

Cannes: Anatomy of a Standing Ovation for The French Dispatch – The New York Times

CANNES, France Wes Anderson has been waiting a long time for The French Dispatch to premiere at the Cannes Film Festival.

A star-studded comedic anthology about the final issue of a literary magazine, The French Dispatch was meant to debut here last year until the pandemic prevented the festival from being held. Instead of putting his movie out in the interim, Anderson held on to it for another year, and at Monday nights glitzy Cannes premiere, he finally got his wish.

So did the film festival. Cannes runs mainly on auteur worship and movie stars, and The French Dispatch offered heaping helpings of both. Cast members, including Timothe Chalamet, Bill Murray, Tilda Swinton, Benicio Del Toro and Owen Wilson, all turned out in support of Andersons film, contributing to what is almost certainly the biggest movie premiere that has been held since the pandemic began.

Cannes responded in kind, and the audience at the Grand Thtre Lumire offered The French Dispatch a nine-minute standing ovation after the closing credits rolled. These epic-length orgies of applause are one of the festivals best-known quirks, but to outsiders, the ovations must be baffling: Does the audience really stand up and clap for that long? Wouldnt that get old fast?

Let me explain how a Cannes standing ovation works, using last nights standing O for The French Dispatch as the minute-by-minute model. Its an ovation that Anderson must have been anticipating for over a year, even if it appeared that he wanted it to end as soon as it began.

1 second in: The credits end, the lights go on, and the cheering audience gets to its feet. A cameraman scurries toward the middle of the theater, where Anderson and his cast are sitting. As he films them, the image is simultaneously broadcast on the Lumires big screen, which gooses the crowds applause even further.

6 seconds in: Though Anderson has risen from his seat, the rest of his cast pointedly stays seated. Nervous, he tries to coax them to stand alongside him, but the actors hold fast: They want Anderson to have his own moment where he can be singularly applauded for his work.

36 seconds in: A half-minute of adulation is about all the visibly uncomfortable Anderson can stand. To his right are Chalamet and the actress Lyna Khoudri, who play French revolutionaries in the film, and Anderson pleads with them to stand up. They begin to, but when Chalamet looks around and sees that no other actor has risen, he stays in his seat.

45 seconds in: Murray stands up and waves to the cheering audience. You can see the rest of the cast doing mental calculations: Well, if Bill Murray is going to stand, then I guess its time to get up. They all rise.

1 minute and 10 seconds in: Murray pulls out a fan and begins to whip cool air at his director. Hey, if the standing ovation is going to go on for several minutes, you might as well sprinkle in some comic bits to pass the time.

1 minute and 30 seconds in: The actor Mathieu Amalric pulls out his iPhone and starts recording a video of the cast. Fitting, since everyone else in the Lumire has an iPhone trained on them, too.

1 minute and 50 seconds in: Swinton goes down the line of her co-stars, giving del Toro and Adrien Brody double kisses on the cheek. Let me attempt to describe Swintons outfit, which consists of a satiny pink blouse, glittery green sleeves and an orange skirt: She looks like the most glamorous fruit plate youve ever seen.

2 minutes in: How can a standing ovation at Cannes possibly sustain itself past two minutes? Heres the trick: The Lumire cameraman, who has previously been recording a wide shot of the cast, now moves to sustained close-ups of each actor. This allows the audience to give each of the performers their own round of applause, and its also why Cannes films with a large ensemble tend to get longer ovations.

2 minutes and 20 seconds in: While the camera is panning from a close-up of Amalric to Khoudri, Brody races from his place at the very end of the cast lineup and heads to where the action is. He hugs Amalric, who is near the front of the line, and the camera pulls back to cover him.

2 minutes and 37 seconds in: Now Chalamet gets his close-up. Thank you, Chalamet says as the audience applauds wildly. He then points to Anderson, encouraging the cameraman to film him instead.

2 minutes and 55 seconds in: Anderson is standing with Wilson and seems wholly uninterested in enduring another half-minute of the audiences prolonged attention. The camera instead locates Swinton, a Cannes veteran who is in three films here this year. Though she is a seasoned pro at accepting a standing ovation, Swinton shakes her head no and points to her director. Eventually, she takes the initiative and pushes the camera toward Anderson herself.

3 minutes and 23 seconds in: The cameraman lingers on a close-up of Anderson, which whips the tired crowd into another round of whoops and cheers. But its clear the director doesnt know what to do with himself when hes the sole focus of the frame. Hes saved by Murray, who comes in for another hug.

3 minutes and 53 seconds in: Brody leans in to kiss Anderson on the cheek and tousles his hair. We are not even halfway through this thing.

4 minutes and 30 seconds in: Swinton takes the taped Tilda Swinton placard from her seat and affixes it to the back of Chalamets silver jacket. We have reached the improv-comedy portion of the night.

5 minutes and 25 seconds in: After locating del Toro at the end of the lineup of actors, the cameraman has now fulfilled his obligation to let each of the performers have their own solo session of applause. So what will keep the ovation going? Cast mischief. The camera drifts back to Chalamet, who hides his face with the Tilda Swinton sign. Swinton snatches it from his hands and tapes it onto his back again, where it belongs.

5 minutes and 50 seconds in: Now hugging Brody, Chalamet turns to the camera and makes the L.A. fingers hand gesture. Brody blows a very serious kiss to the camera.

6 minutes and 5 seconds in: Yes, were going into Minute 6. Anderson pulls out a pink handkerchief and wipes his brow. He appears to be teary-eyed.

6 minutes and 35 seconds in: Chalamet turns to Anderson and bows in an Im not worthy salute. The applause is starting to flag a little. Its time to pull out the big guns.

7 minutes and 7 seconds in: Anderson is handed a microphone. He winces and tries to turn it away, but Cannes officials press it into his hands anyway.

7 minute and 15 seconds in: Anderson, who lives in Paris, begins to speak to the audience in French. He calls the premiere un honneur pour moi, but after seven seconds of that, he turns to Chalamet and cracks in English, I dont know what else to say. The audience laughs and Anderson adds, I hope we come back with another one soon. Thank you so much.

7 minutes and 30 seconds in: Andersons short speech was enough to resuscitate the crowd, and the applause surges back to its initial levels.

7 minutes and 50 seconds in: Several French-accented cries of Bravo! are heard as Anderson tucks his long hair behind his ears and scans the audience.

8 minutes and 24 seconds in: Murray goes over to Anderson and suggests that hes ready to leave. Anderson could not possibly agree more, racing up the aisle so quickly that he bumps into the cameraman, who is still filming him.

8 minutes and 40 seconds in: It appears the cameraman has blocked Andersons path. He wont get away that easily! Instead, Anderson is forced to stand in the aisle and absorb even more applause and encouraging whistles from the crowd. The expression on his face is somewhere between an awkward grimace and pure, stunned joy, which is what nearly nine minutes of a standing ovation will do to you.

9 minutes in: The cameraman relents and allows Anderson to move forward. As the director and his cast leaves the theater, the ovation finally subsides. The French rush outside to smoke, the Americans rush outside to tweet, and in a few different languages, I hear one plaintive question: Is there an after-party?

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Cannes: Anatomy of a Standing Ovation for The French Dispatch - The New York Times

Penn State football: Anatomy of a position group The wide receivers – rivals.com

Starting this week, Blue White Illustrated will begin a new series breaking down each Penn State position group and how it was constructed, also touching on its outlook ahead of the 2021 season and looking further forward. We continue the series with Penn State's wide receivers

Quarterbacks

Running Backs

Tight Ends

The contrast between how Penn State fans felt about their wide receivers heading into the 2020 season and how they feel about that position group with the 2021 season looming couldn't be much greater.

Concern and trepidation have been replaced by confidence and assurance with Penn State looking toward a season-opening date with Wisconsin.

Jahan Dotson emerged from his status as a relative unknown to become the Big Ten's leading receiver in 2020. His decision to return for another season in Happy Valley was a huge boost to everyone of a blue and white persuasion.

Similarly, Parker Washington had a fantastic season for a true freshman, finishing with 489 yards and six touchdowns in nine games.

It was an impressive campaign from that duo and one, in Washington's case, specifically that may have been even better under normal circumstances, considering he had almost no chance to develop a rapport with Sean Clifford before the season.

"It was a weird year in general," Clifford said. "Jahan obviously had been here, but with the new guys, I really didn't get a to meet them or throw to them until fall camp because we were separated because of COVID and then we were in different camps and we were practicing differently.

"The first time I threw to Parker was actually two weeks before the first game."

With those two standouts in the fold, there's reason to be excited about Penn State's top two options at that position.

The rest, though, is still up in the air.

There's no clear-and-obvious option to take up the mantle as Penn State's third wideout in 2021. Could it be one of the veterans like Cam Sullivan-Brown or Daniel George? Will a younger player like KeAndre Lambert-Smith emerge?

It's anyone's guess, and Penn State's staff will have to sort out Penn State's depth to ensure that the complementary pieces around Dotson and Washinton can do their part to ensure this group has a successful season.

So, now that we've covered the present, how does the future look at wideout for the Nittany Lions?

That could partially depend on what Lonnie White Jr. decides.

The four-star wideout was drafted this week by the Pittsburgh Pirates, and must now decide whether to pursue professional baseball or play for the Nittany Lions.

Penn State has pulled in at least one four-star or better wide receiver in every recruiting class since 2017, meaning the Nittany Lions should have plenty of options to choose from down the line.

Their 2022 class at that position looks like it could be special, too.

Anthony Ivey and Kaden Saunders are both exciting prospects who could have a big future with the Nittany Lions. Mehki Flowers fits that bill, too, but it seems likely that his future with Penn State is played out at the safety position.

There are plenty of variables within the room including the future of Marquis Wilson, who took snaps at wideout this spring after starting his Penn State career as a corner.

But even with some uncertainties, the future at wideout for the Nittany Lions looks bright.

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Penn State football: Anatomy of a position group The wide receivers - rivals.com

Anatomy of a Goal: Miguel Berry secures a Hell is Real draw – Massive Report

Welcome to the Anatomy of a Goal, where each week we dissect one goal (or near goal) from a previous Columbus Crew match.

For match 12 of the 2021 MLS season, the first Hell is Real match at FC Cincinnatis TQL Stadium, we take a look at Miguel Berrys first goal for the Crew that gave Columbus a come-from-behind draw against the teams southern Ohio rivals.

Heres a look at the goal from the Black & Golds young striker.

The first Hell is Real Derby of 2021 could not have started any better for the home side, still looking for their first win at TQL Stadium. Within the first 30 seconds of the match, FC Cincinnati was able to grab a 1-0 lead. Luciano Acosta doubled the lead in the 24th minute, and a Harrison Afful red card from a second yellow card in the 42nd minute saw the Crew looking up at the scoreboard down a man and two goals with just over one half to play.

Lucas Zelarayan worked some more of his magic to cut the lead to one goal just before halftime. FCC held the possession edge in the second half but the Black & Gold still managed to create some attacking chances despite playing down a man.

Berrys game-tying goal begins from a bit of Cincinnati possession. Liga MX and MLS veteran Edgar Castillo possesses the ball under slight pressure from Berry and can either play a long pass up the field toward Brenner, hit a quick touch pass to Yuya Kubo, make a square pass to Caleb Stanko play a drop-back pass to Gustavo Vallecilla.

Castillo opts for the more difficult pass forward to Brenner, who is marked by Saad Abdul-Salaam.

Castillos pass is off target, allowing Abdul-Salaam to easily intercept the ball.

Abdul-Salaam carries the ball forward and finds himself with five options. He can play a pass up the sideline to Berry, attempt a long pass forward to Crew debutant Erik Hurtado, carry the ball forward, play a short pass into the path of Isaiah Parente or try a square pass to Darlington Nagbe.

Abdul-Salaam continues the quick counter attack, opting for the attacking pass to Hurtado.

Hurtado takes an awkward touch but is able to collect the under Stankos defensive pressure. Notice Zelarayan, the most dangerous player on the field, standing totally unmarked in the middle of the field.

Hurtados collection takes him around Stanko and he uses his next touch to hit a long drop pass back to Aboubacar Keita, with Zelarayan still unmarked nearby.

Keita approaches the ball under pressure from Brandon Vazquez. The Columbus Homegrown player must use his first touch to either dribble around Vazquezs pressure or make one of three passing choices: a touch forward to Nagbe, a long pass forward to an un-marked Zelarayan or a square pass to Pedro Santos.

Keita hits a well-placed, but a bit heavy, first-touch pass right to Zelarayan.

Zelarayan takes a moment to collect the ball before starting his turn upfield. The Black & Gold attacker is pressured by Franko Kovacevic (21) on one side while Stanko will attempt to prevent him from turning toward the FCC goal.

Zelarayan beats Kovacevics light pressure and then is able to jump over Stankos outstretched leg.

The Crews No. 10 turns toward the goal and surveys his options. He can either play a diagonal pass forward to Abdul-Salaam, try a long, through ball to Berry, attempt a pass between Kubo and Stanko to Nagbe or attempt to carry the ball forward.

Zelarayan spots Berry and hits a perfectly weighted through ball into the path of the young striker.

Rather than collect the ball and slow down his momentum, Berry lets the ball run out in front of him and maintains his pace. This savvy move allows Berry to easily beat Vallecilla and turn toward the goal.

The ball, perfectly paced by Zelarayan, rolls in front of Berry who finds himself chasing the ball with no outfield defenders between him and the goal.

Berry approaches the ball and must decide what to do with his first touch as Kenneth Vermeer attempts to close down the play. The Columbus striker can use his first touch to take a touch around Vermeer, take a shot over Vermeer toward the goal or play a quick pass into the path of Hurtado.

Vermeer slides into his path and Berry decides to shoot a chipped shot over the FC Cincinnati goalkeeper.

Vermeer has gotten low as Berry chips a low-driven shot over him.

Vermeer is unable to get a touch on the ball.

Berrys shot beats Vermeer and takes a bounce in the middle of the six-yard box, sliding...

...into the back of the net!

Findings:

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Anatomy of a Goal: Miguel Berry secures a Hell is Real draw - Massive Report

Anatomy of . . . India batter Shafali Verma | Sport | The Sunday Times – The Times

FactfileAge 17Birthplace Rohtak, Haryana

Tests 1Batting avg 79.5Highest score 96

ODIs 3Batting avg 26Highest score 44

T20 internationals 25Batting avg 27.7Strike rate 145.19 (Ranking: No 1)

Veggie is bestVerma used the enforced break in the cricket schedule to work on her fitness and physicality, adopting a strict vegetarian diet and cutting out all snacks and junk food which helped her lose six kilos in weight and increase her muscle denseness. I didnt know much about these things [diet and nutrition] but when you play international cricket, the fitness trainers constantly work on your bodies. I do miss my pizza and Doraemon though she said. There is also the small matter in the next year, of her

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Anatomy of . . . India batter Shafali Verma | Sport | The Sunday Times - The Times

The Anatomy – and End – of Daniel Negreanus Tournament Futility – PocketFives

Daniel Negreanu was all smiles. In front of him were multiple stacks of Euros and in his hand was a World Series of Poker bracelet the sixth of his career. He was posing for winners photos in front of a backdrop that included the Eiffel Tower.

It was October, 2013, and Negreanu had just won the 25,000 High Roller at WSOP Europe and in the process, claimed his second WSOP Player of the Year award. It was his 40th career live tournament victory and he was just a month away from being inducted into the Poker Hall of Fame. Little did he know what was coming.

It was another 2,821 days nearly eight years before Negreanu won another live tournament. Posed for another winner phot. That streak of tournament futility ended on Tuesday, when he defeated rising star David Coleman heads up to win Event #7 ($50,000 NLHE) of the PokerGO Cup. Between that memorable night in Paris and Tuesday night in Las Vegas, there were multiple close calls through the years. And millions of dollars lost.

The time between victories included ten runner-up in-the-money finishes. A feat deemed so spectacular that Doug Polk recently created a video about it. Outside of finishing one spot behind Phil Hellmuth in a Poker After Dark sit-n-go, most of Negreanus nine other second place results came in high-profile spots.

The first two came at the 2014 World Series of Poker in Las Vegas. First, he lost to Paul Volpe heads up in the $10,000 No Limit Deuce event. Then came what was arguably the most high-profile runner-up finish in poker history. Negreanu lost to Daniel Colman in the second-ever $1 million buy-in Big One for One Drop. He walked away from that with $8,288,001 in earnings while Colman took home $15,306,668 for his win.

In April 2017, he posted back-to-back runner-up finishes in a pair of $25,400 buy-in Mixed Game High Roller events at the Bellagio before returning to the WSOP that summer. There he finished behind Abe Mosseri in the $10,000 Omaha Hi-Lo Championship event. In November of that year, Dan Smith took home $1,404,000 for beating Negreanu heads up in a $100,000 Super High Roller at the Bellagio. His Poker After Dark second place finish came a month later.

In 2018, he finished second to Justin Bonomo in the $300,000 Super High Roller Bowl. His most recent runner-up finishes came at the 2019 WSOP. He lost to John Hennigan in the $10,000 Seven Card Stud Championship before falling to Keith Tilston in the $100,000 Super High Roller event.

The difference between winning and finishing second in all of those events was worth $11,088,018.

Poker fans were given a treat when some trash talk on social media between Negreanu and longtime nemesis Polk turned into the closest thing to heads-up-for-rolls weve ever seen between two elite players. Negreanu and Polk agreed to play 25,000 hands of $200/$400 No Limit Holdem online and Polk promised that he was going back up the truck and put a financial hurt on Negreanu.

Polk did just that. While it wasnt a tournament loss, over the four months of action, Polk beat Negreanu at the tables for $1.2 million and hung another L on Negreanus board.

Between April and June, Negreanu squared off with Phil Hellmuth three times on PokerGOs High Stakes Duel II, a heads up freezeout where the buy-in doubles for each consecutive match. In the first match, which had a $50,000 buy-in, Negreanu had Hellmuth facing a 20-1 chip deficit only to have the 15-time WSOP bracelet winner mount a comeback to take the opening match. A month later, with the buy-in now $100,000, Hellmuth defeated Negreanu in the second match. The third match, which cost $200,000 to enter, also went to Hellmuth and as was his option after winning three straight, Hellmuth exited stage right with $350,000 of Negreanus money.

The 10 runner-up finishes, the three losses to Hellmuth, and the seven-figure loss to Polk werent the only close calls that Negreanu had in between wins. He came third nine times, fourth three times, and wound up in fifth place in three other events.

He also suffered the indignity of being named the 2019 WSOP Player of the Year in November 2019, only to have the win overturned after a data-entry error erroneously awarded him points in an event he didnt cash in. Once the numbers were re-run, he wound up in third place.

It wasnt all bad news for Negreanu. Between the WSOP Europe title and the PokerGO Cup tournament win, he finished in the money 99 times and had total earnings of $21,860,360.

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The Anatomy - and End - of Daniel Negreanus Tournament Futility - PocketFives

‘Grey’s Anatomy’ Fans Argue That Alex Karev’s Violent Streak Never Actually Went Away – Showbiz Cheat Sheet

Greys Anatomy fans have fallen in love with many characters throughout its 18 seasons. Unfortunately, several of them left the series for one reason or another. However, the shocking ending for Dr. Alex Karev (Justin Chambers) left some supporters speechless.

Additionally, the exit didnt surprise longtime fans for one obvious reason.

Greys Anatomy premiered on ABC on March 27, 2005. When the show came out, viewers met surgical interns Alex, Meredith Grey (Ellen Pompeo), Cristina Yang (Sandra Oh), George OMalley (T.R. Knight), and Izzie Stevens (Katherine Heigl).

RELATED: Greys Anatomy Fans Have Strong Opinions On How Showrunners Are Dealing With Justin Chambers Mysterious Exit

From the beginning, Alex stood out from the crowd. The surgeons arrogant attitude and harsh comments made him the hospitals resident villain. As the show progressed, though, Alex showed more of his softer side. When Alex and Izzie started dating in season 4, Alex proved his love to her when she received a cancer diagnosis. Soon, they got married, but Izzie eventually left her husband after thinking he got her fired from the residency program in season 6.

Eventually, Alex met Jo Wilson (Camilla Luddington). After several years of dating, they married during season 14. However, shortly after their second wedding, Alex left Jo for Izzie. In season 16, episode 16, Leave A Light On, Alex explained to Meredith, Jo, Dr. Miranda Bailey (Chandra Wilson), and Dr. Richard Webber (James Pickens, Jr) that he left Seattle to be with Izzie and their kids. The exit happened after Chambers left Greys Anatomy in Jan. 2020.

RELATED: Greys Anatomy: Ellen Pompeo Reacts to Justin Chambers Exit on Twitter and It Will Break You

Although Chambers didnt appear in Alexs final episode, the episode paid homage to his 16 seasons. After Leave A Light On aired, many fans were surprised to see Alexs character development shift fast. While leaving one woman for another was something the character would do in earlier episodes, viewers watched him change significantly as the seasons progressed.

In a Reddit thread, one user suggested that Alexs abrupt exit wasnt surprising. According to the fan, Alex never changed his ways throughout his 16-season run.

Alexs character development wasnt as great as people think, the fan shared. He was still beating people up close to the end of his run.

After pointing out Alexs explosive fight with Dr. Andrew DeLuca (Giacomo Gianniotti) in season 12, they also said his exit was complete garbage. However, they ended the comment by stating that Izzie and Alex were a much better pair than Jo.

RELATED: Greys Anatomy: Can the Show Survive Without Alex Karev?

Following Alexs exit, some Greys Anatomy fans still wish he never left Jo or Meredith behind. Although Greys showrunner Krista Vernoff admitted that the characters farewell felt like a betrayal to some fans, she believes his story received a proper ending.

It felt OK to our writers, who also grew up with these characters, she told Variety. These people are real to us, too. And that felt like the way we could say goodbye to Alex.

Currently, there are no plans for Alex to come back to Grey Sloan. Since Greys Anatomy, Chambers has been laying low. However, the actor recently signed on to play Marlon Brando in Paramount+s upcoming limited series, The Offer (via Comingsoon).

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'Grey's Anatomy' Fans Argue That Alex Karev's Violent Streak Never Actually Went Away - Showbiz Cheat Sheet

Anatomy of an outbreak: How COVID-19 overran this Ontario prison – TVO

On April 14, 2020, employees at BramptonsOntario Correctional Institute received a memo informing them that a co-worker had tested positive for COVID-19. It is important to note that the individual did not experience symptoms while at work, wrote Mark Parisotto, director of the central region for the Ministry of the Solicitor Generals Institutional Services Division. The employee remains in self-isolation and is following protocols recommended by health authorities.

Employees were advised to wash their hands often, sneeze into their sleeve, avoid touching their face and contact with sick people, and stay home if they were ill. They were told that all work areas the employee had come in contact with had been cleaned and that common area touch points were being disinfected twice a day. We have been advised that no additional precautions are necessary at this time, Parisotto wrote. Your health and well being are priorities for my management colleagues and I.

The ministry, which operates provincial correctional facilities, had been made aware of the positive case three days earlier. On April 15, Peel Public Health declared an outbreak,following two lab-confirmed and one probable case of COVID-19 in correctional officers.On April 20, OCI closed. Inmateswere moved firstto Toronto South Detention Centre and then, in June, to the Maplehurst Correctional Complex. Both facilities later experienced outbreaks of their own.

You can count on TVO to cover the stories others dontto fill the gaps in the ever-changing media landscape. But we cant do this without you.

A ministry spokesperson confirms that, whenOCI closed, 91 of its 109 inmates had tested positive. According toAdam Cygler, a social worker at OCI and former president of OPSEU local 229, the union that represents its correctional workers, 32 of the roughly170staff and 11 family members of staff were either confirmed or presumed to be positive for COVID-19. The ministry did not respond to TVO.orgs request for verification of these numbers.

Itwas then the most significant outbreak at any provincially run prison in Ontario. At a time when public experts were adjusting guidelines to match the developing knowledge of the disease, it became a flashpoint for concerns about worker and inmate safety and managerial indifference and fuelled questions about government policy that have echoed throughout the pandemic.

Staff had raised concerns about the potential for COVID-19 spread within the facility months before the outbreak was declared. We foresaw this becoming an issue in the correctional institutions, says Ryan Graham, co-chair of OPSEUs Provincial Joint Occupational Health and Safety Committee. He says the committee recommended as early as January 2020that the ministry implement active screening methods in its facilities but that the ministry pushed back, stating that it wasnt the time for such measures. One of the overarching things we have always heard is were not there yet, says Graham. If I have to fault the ministry on anything, it is not being proactive enough. Weve been very reactive.

A ministry spokesperson tells TVO.org via email that from the beginning of the COVID-19 pandemic, the ministry has been working with its partners to keep provincial correctional facilities and our communities safe.

Inside OCI which, according to the ministry, provides specialized and intensive treatment for substance abuse, sexual misconduct, impulse control and anger management inmates beds line the walls of large, communal dormitories. Common areas include a day room, bathrooms, a dining hall, and a gym. We are, apart from eight cells, 99 per cent a dorm setting, Cygler says. And individuals interact in a very community-style way.

Teesays that,compared to Toronto South Detention Centre,OCI was a breath of fresh air. Tee, who asked that TVO.org use only his first name to protect his anonymity, had been incarcerated at TSDC since May 2019 andwas transferred to OCI in January 2020.

I heard that the place is more of a rehabilitation centre as opposed to other provincial jails, because they dont really offer much. OCI has a bunch of different programs, saysTee. But in March, things changed. Tee describes the month leading up to the outbreak as chaotic. Many residents were angry because, as they learned about COVID-19 spreading across the province on the news, they began asking for such protective measures as masks or gloves according to Tee, they were ignored. It was like they didnt care what we had to say because were in orange, he says.

OCI, like many institutions, did have its own influenza-preparedness plan. TVO.org obtained a copy of this plan, dated March 2020, through a freedom-of-information request. The document recommends such precautions as PPE use, social distancing, and restrictions on inmate transfers. But staff members andTeesay that they werent implemented properly.

We were told that they wouldnt activate the pandemic plan until there was an outbreak, and our response was, Well, then its too late, says Cygler. The ministry did not respond to direct and repeated questions about when the facilitys pandemic plan was activated.

The facility didnt mandate social distancing, considering it a personal choice, says Jamie,a correctional officer who,concerned about potential professional ramifiations, requested that theirname and personal pronoun bechanged to protect their anonymity and distancing was not observed during a staff-appreciation event held at the facility in late March. A spokesperson for the ministry confirms to TVO.org that a drop-in staff event was held on March 25 but states that distancing measures were in place. Prior to the OCI outbreak, Jamie notes, management had placed posters and floor markers throughout the facility to indicate appropriate distances, as set out in public-health guidelines.

Tee says that, while such markers were in place, inmates did not observe social distancing during meals: It was kind of weird, because theres a lot of contradictions that were happening in that time as far as what we can and cant do.

Jamie, who says they worked with the employee whod been the first reported case, says management did not inform them of the possible exposure and that they found out only when the infected co-worker told them they had a fever. TVO.org asked the ministry when management learned that a staff member was exhibiting symptoms; the ministry responded that it does not publicly discuss confidential human-resources matters, especially in cases involving personal health information.

Mask-wearing guidelines were then in a state of flux: Peel Public Health confirmed to TVO.org that it recommended universal masking at OCI during the outbreak but noted that, at the time, there was noprovincial guidance for the management of COVID-19 in congregate settings.It wasnt until April 6 thatthe federal government recommendedthat people use non-medical masks in situations where physical distancing was difficult or impossible, and it wasnt until July 10 thatBrampton mandated masksin all public indoor spaces.

At OCI, a March 17 memo from deputy superintendent of administration John Hasted indicates that correctional officers conducting COVID-19 screenings must wear surgical masks, gloves, and eye protection while within two metres of an unscreened inmate. He adds, however, Staff/residents who are not involved in the screening and can be 2 metres away before and during the screening do not need to have a mask and eye protection.

Some staff, though, wanted more routine mask-wearing and say they were both ignored and ridiculed.

In March, there were hundreds of N-95 masks in the facilitys training office, says Brett, a corrections officer who, concerned about potential professional ramifiations, requested that TVO.org change theirname and personal pronounto protect their anonymity. Brett says, though, that when they asked whether they could get one to wear while admitting and discharging inmates, they were told by the business administrator that it was not issuing N-95 masks. OCIs pandemic plan indicated that PPE, including N-95 masks, were available in inventories on site in quantities estimated to last approximately eight weeks.

After Brett threatened to file a complaint, they say, OCI offered a surgical mask, but Brett told their superiors they worried that a surgical mask wouldnt provide adequate protection. Management told them, Brett says, that the N-95 masks had expired and that the straps might break. In the past, Brett says, N-95 masks had been distributed to those likely to come in contact with someone who had tuberculosis, which can spread through droplets. In March, though, Brett says, They laughed at me when I asked for the N-95, so what can you do? It was almost like a joke. They took it very lightly.

Jamie, who tested positive two days after OCIs closure on April 20, says staff were not required to wear masks until the outbreak was declared on April 15: We were not required to wear a mask in the facility prior to anybody getting sick.

Prior to the outbreak, Jamie attempted to wear their own mask, but Jamie says that asupervisor told them that they would be subject to disciplinary action for doing so, because masks would frighten the inmates. Jamie says, however, Inmates were asking why we werent wearing masks. TVO.org repeatedly asked the ministry whether workers could face disciplinary action in this circumstance but received no response.

I think at the time, the administration was concerned about the inmate population panicking or becoming fearful, says Cygler. And what we were saying is sort of the opposite. I think they would be reassured by seeing us take the appropriate precautions.

Tee says that inmates repeatedly asked correctional workers why they werent wearing masks: There was a lot of back and forth between the residents and the staff we knew that, because we were pretty much closed off to society, the only way for anyone to get COVID was through someone from the outside, and nobody there wore any PPE whatsoever.

On April 9, 2020, superintendentFelecia Hooper issued a memo stating that staff could wear their own masks in situations where doing so was not required under public-health guidelines. When a staff member enters a situation where PPE is required, they must remove their personal mask and use ministry-issued PPE only, Hooper wrote in the memo, which TVO.org also obtained through a freedom-of-information request.

She stated that staff members would be required to sign a waiver toconfirm they are accepting the risk of wearing a personal mask and to have the mask visually inspected by a management team member each day to ensure that it had no offensive markings, did not present a known and avoidable elevated security risk to the wearer or others, and looked like an N-95 or surgical mask.

Still, until the outbreak was declared at OCI, staff say, management did not provide masks. Work refusals filed by OCI correctional staff to the Ministry of Labour indicate that they were unwilling to transfer sick inmates unless the workers had masks; one officer wrote on April 14 that they wererefusing to go to a unit with sick inmates unless masks were provided by their employer.The ministry conducted a field visit that day and determined thatOCI was meeting its responsibilities under the Occupational Health and Safety Act.

OCI wasnt the only institution where staff were refusing to work. According to ministry data obtained by TVO.org, 48 refusals were filed last year between March 11 and April 20 at various correctional facilities across the province. Most involved staff alleging that preventative measures werent being taken. One refusal, from the Ottawa-Carleton Detention Centre on March 27, states that the facility had not provided or posted a contingency plan to quell panic. Another refusal, from South West Detention Centre, dated March 30, indicates that a staff member was refusing to work in a unit where they could not practise physical distancing; they stated that they would work in the unit if they were given an N-95 mask but that their request for one had not been granted.

Staff were truly concerned for their health and safety about entering the workplace, says Graham.

The Ministry of Labour, in a statement, saysit determined the institutions were following the appropriate health and safety protocols. A ministry representative tells TVO.org via email that when a work refusal is not upheld, the workers concern is investigated as a complaint.In some cases, the health & safety concerns were resolved internally by the workplace parties with assistance from a MLTSD health and safety inspector if required.

TVO.org asked the ministry at what point it became a requirement that staff members and inmates wear PPE throughout the facility but did not receive a response. On April 22 a week after the OCI outbreak was declared OPSEU and the ministryarrived at a dealthat required all staff and visitors at Ontario prisonsto wear surgical masks.

In an April 15 memo, Deputy Superintendent John Hasted announced that the institution was going into lockdown; movement would be restricted and four units placed on so-called droplet precautions which, according to April 2020ministry guidelinesare required for inmates who have symptoms, are persons under investigation for influenza-like illness, or have identified risk factors such as close contact or travel history.

Residents that were showing cold-like/flu-like symptoms have been isolated in the segregation area, he writes. Meals were no longer to be eaten in the cafeteria, and all staff working in Droplet precaution areas will be issued PPE.

Deputy Superintendent Donna Butler released a memo the same day, indicating that OCI had suspended all programming, including House Meetings, Peer Reviews, Recreation, Library and treatment specific programs, etc., until further notice.

Howard Sapers, who served as the Ontario governments independent adviser on

corrections reform from 2017 to 2018, says corrections, like every other sector, was unprepared for the risk that COVID-19 posed. What caught the rest of the world off-guard also caught corrections off-guard, he says. And Ontario, like every other correctional system, was catching up to the virus as the risk of infection was growing.

Monica Hau, Peel Regionsassociate medical officer of health notes that, at the time of the outbreak, everything was pretty new still. Understanding of the disease and its transmission was rapidly evolving, she says, adding, For example, we were just starting to get a better understanding of the role of pre-symptomatic and asymptomatic transmission. And infection-prevention and -control measures that are now so routine were actually relatively new at that time.

But the union was clear on who needed to shoulder the blame. In anApril 20statement, OPSEU president Warren Thomas said the outbreak had resulted from management incompetence. For weeks, he said, the people running our jails have ignored the direction of public health officials and know-how of frontline workers who want to keep our jails as safe as possible.

Graham says that the union and ministry had more frequent discussions about safety measures after the OCI outbreak. And the ministry tells TVO.org via email that it has made important operational changes across all provincial correctional facilities; for example, newly admitted inmates are separated from the general population for 14 days, all staff are required to wear a mask and appropriate eye protection while at work, and staff and visitors receive temperature checks. Asked when these measures were introduced, the ministry did not respond.

Since OCI closed, however, outbreaks have been reported in Maplehurst, TSDC, the Thunder Bay District Jail, the Thunder Bay Correctional Centre, the Stratford Jail, theCentral East Correctional Centre, and, most recently, the North Bay Jail. Two other facilities have temporarily closed as a result of outbreaks the Stratford Jail in February and the North Bay Jail in June. According to aresearch projectled by the University of Winnipegs Kevin Walbyand the University of OttawasJustin Pich, Ontario has reported the highest number of correctional cases of all the provinces tracked, with2,240 as of June 29

Sapers says that the very design of jails and correctional facilities makes them more susceptible to spread. Youve got a high concentration of people living in very close quarters, often with less than optimal fresh-air circulation, and you also have a tremendous amount of movement in and out of the facility, he says, adding that, as a result, it can be hard to properly apply such public-health guidelines as social distancing. In his view, the most effective way to limit spread is to reduce the number of inmates: Depopulation of carceral centres, jails, and prisons has proven to be more successful than trying to just take a public-health approach inside the jail.

Early on the pandemic, he says, the government did pursue that strategy with a real sense of urgency.In March 2020, thegovernment announcedthat it wouldissue temporary absences beyond the current 72-hour maximum and allow for early release of those inmates near the end of their sentences.

However,a reportfrom the Canadian Civil Liberties Association, produced in partnership with the Centre for Access to Justice and the Criminalization and Punishment Education Project, found that, by early 2021, provincial jails and federal prisons in Ontario and other provinces were detaining more individuals than they had been at the end of the first wave. Statistics Canada data included in the report shows that Ontarios correctional population steadily increased from 5,811 in June to more than 7,000 in March 2021.

I think that they took their foot off the gas, diversion- and decarceration-wise right when, arguably, the need to ramp up those efforts increased, says Pich, an associate professor of criminology.

Graham recommends an additional measure to limit COVID-19 spread: changing the way updates are shared with correctional facilities.

Currently, he says, information about those who have come in contact with a positive case goes from the local institution to the ministry, which sends it to public health. The information gathered from contract tracing is then sent back from public health to the ministry and from there to the facilities. Contact tracing, Graham says, can take days or weeks: Theres a lot missed in the meantime. (Peel Public Health says it received word of the first positive test from OCI on April 10 but it wasnt until April 13 that it received the relevant contact list and began tracing.)

Graham would like to see more direct communication between local public-health units and facilities: A simple process would be, a staff member is identified positive, the joint health and safety committee does a health and safety investigation. They gather that information and give it to public health, public health does contact tracing, they contact individuals, and the summary gets sent back to the institutions. And that can be done within, arguably, hours.

Vera Etches, Ottawas chief medical officerof health, has suggested three ways to help prevent future outbreaks in correctional institutions. In aJune 21 letterto Ontarios solicitor general, she recommends the governmentensure that facilities have sufficient capacity to isolate inmates and that isolation periods are adhered to; establish practices to monitor the vaccination coverage rates of staff and inmates on a weekly basis to guide on-site immunization efforts; andfully implementrapid testing prior to transfer to or from other correctional facilities. (Etches congratulated the ministry for introducing a pilot program for rapid testing but called for its full implementation.)

For OCI and other correctional facilities across the province, vaccination remains the most effective measure when it comes to reducing spread, Hau says: I think its been very clear,the success in long-term-care homes Im certainly hopeful that correctional facilities can achieve the same rate of vaccination. At the end of May, the ministry told TVO.org that the current vaccine uptake among inmates [was] approximately 50 per cent. However, in June,theToronto Starreportedthatinstitution-level vaccination data obtained from the ministry and local public-health units showed that vaccination rates varied widely between facilities from 100 per cent at the Algoma Treatment and Remand Centre in Sault Ste. Marie to 13 per cent at the Toronto East Detention Centre.

The Agenda With Steve Paikin, April 6, 2020: COVID-19 and the corrections system

OCI has now been closed for more than a year. Theres no indication of when it will reopen. The ministry saysit is carefully considering options before making any plans to reopen OCI.

Tee say that, since his release last November, hes realized how difficult it can be to enforce public-health measures, no matter the setting. But he still believes more should have been done at OCI, especially because of its layout: The guy that sleeps next to me is less than two feet away from me. Other former inmates feel the same way he does, he says: They dropped the ball as far as protecting us.

According to Cygler, The impact the outbreak has had on staff and resident morale and trustthats going to take a long time to repair.

Jamie says:They completely ignored the fact that we are a unique setting, Jamie says. They let us down.

Correction: An earlier version of this article misspelledFelecia Hooper'sname. TVO.org regrets the error.

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Anatomy of an outbreak: How COVID-19 overran this Ontario prison - TVO