All posts by medical

Neuroscience

Scientists show what loneliness looks like in the brain – Newswise

Newswise This holiday season will be a lonely one for many people as social distancing due to COVID-19 continues, and it is important to understand how isolation affects our health. A new study shows a sort of signature in the brains of lonely people that make them distinct in fundamental ways, based on variations in the volume of different brain regions as well as based on how those regions communicate with one another across brain networks.

A team of researchers examined the magnetic resonance imaging (MRI) data, genetics and psychological self-assessments of approximately 40,000 middle-aged and older adults who volunteered to have their information included in the UK Biobank: an open-access database available to health scientists around the world. They then compared the MRI data of participants who reported often feeling lonely with those who did not.

The researchers found several differences in the brains of lonely people. These brain manifestations were centred on what is called the default network: a set of brain regions involved in inner thoughts such as reminiscing, future planning, imagining and thinking about others. Researchers found the default networks of lonely people were more strongly wired together and surprisingly, their grey matter volume in regions of the default network was greater. Loneliness also correlated with differences in the fornix: a bundle of nerve fibres that carries signals from the hippocampus to the default network. In lonely people, the structure of this fibre tract was better preserved.

We use the default network when remembering the past, envisioning the future or thinking about a hypothetical present. The fact the structure and function of this network is positively associated with loneliness may be because lonely people are more likely to use imagination, memories of the past or hopes for the future to overcome their social isolation.

"In the absence of desired social experiences, lonely individuals may be biased towards internally-directed thoughts such as reminiscing or imagining social experiences. We know these cognitive abilities are mediated by the default network brain regions," says Nathan Spreng from The Neuro (Montreal Neurological Institute-Hospital) of McGill University, and the study's lead author. "So this heightened focus on self-reflection, and possibly imagined social experiences, would naturally engage the memory-based functions of the default network."

Loneliness is increasingly being recognized as a major health problem, and previous studies have shown older people who experience loneliness have a higher risk of cognitive decline and dementia. Understanding how loneliness manifests itself in the brain could be key to preventing neurological disease and developing better treatments.

"We are just beginning to understand the impact of loneliness on the brain. Expanding our knowledge in this area will help us to better appreciate the urgency of reducing loneliness in today's society," says Danilo Bzdok, a researcher at The Neuro and the Quebec Artificial Intelligence Institute, and the study's senior author.

###

This study was published in the journal Nature Communications on Dec. 15, 2020. It was partially funded by a grant to Spreng and Bzdok from the U.S. National Institute on Aging.

The Neuro

The Neuro - The Montreal Neurological Institute-Hospital - is a world-leading destination for brain research and advanced patient care. Since its founding in 1934 by renowned neurosurgeon Dr. Wilder Penfield, The Neuro has grown to be the largest specialized neuroscience research and clinical center in Canada, and one of the largest in the world. The seamless integration of research, patient care, and training of the world's top minds make The Neuro uniquely positioned to have a significant impact on the understanding and treatment of nervous system disorders. In 2016, The Neuro became the first institute in the world to fully embrace the Open Science philosophy, creating the Tanenbaum Open Science Institute. The Montreal Neurological Institute is a McGill University research and teaching institute. The Montreal Neurological Hospital is part of the Neuroscience Mission of the McGill University Health Centre. For more information, please visithttp://www.theneuro.ca

Go here to read the rest:
Scientists show what loneliness looks like in the brain - Newswise

Neuroscience

FLiCRE: A New Tool for Revealing the Brain in Action – Technology Networks

A new molecular probe from Stanford University could help reveal how our brains think and remember. This tool, called Fast Light and Calcium-Regulated Expression or FLiCRE (pronounced "flicker"), can be sent inside any cell to perform a variety of research tasks, including tagging, recording and controlling cellular functions.

"This work gets at a central goal of neuroscience: How do you find the system of neurons that underlie a thought or cognitive process? Neuroscientists have been wanting this type of tool for a long time," said Alice Ting, professor of genetics in the Stanford School of Medicine and of biology in the School of Humanities and sciences, whose team co-led this work with the lab of Stanford psychiatrist and bioengineer, Karl Deisseroth.

In proof-of-concept experiments, detailed in a paper published Dec. 11 in Cell, the researchers used FLiCRE to take a snapshot of neural activity associated with avoidance behavior in mice. By coupling the FLiCRE snapshot with RNA sequencing, they discovered that these activated neurons primarily belonged to a single cell type, which was inaccessible using genetic tools alone. They then used FLiCRE in combination with an opsin - a protein for controlling neural activity with light developed by Deisseroth - to reactivate those same neurons a day later, which led the mice to avoid entering a certain room. The brain region the researchers studied, called the nucleus accumbens, is thought to play an important role in human psychiatric diseases, including depression.

Modular molecular technology

FLiCRE is made up of two chains of molecular components that respond to the presence of blue light and calcium. This light sensitivity allows the researchers to precisely control the timing of their experiments, and calcium is an almost-universal indicator of cell activity. To get FLiCRE inside a cell, the researchers package it, in two parts, within a harmless virus. One part of FLiCRE attaches to the cell membrane and contains a protein that can enter the cell's nucleus and drive expression of whatever gene the researchers have selected. The other part of FLiCRE is responsible for freeing the protein under certain specific conditions, namely if the concentration of calcium is high and the cell is bathed in blue light.

Whereas existing tagging techniques require hours to activate, the FLiCRE tagging process takes just minutes. The researchers also designed FLiCRE so that they can use standard genetic sequencing to find the cells in which FLiCRE activated. This allows them to study tens of thousands of cells at once, while other techniques tend to require the analysis of multiple microscopic images that each contain hundreds of cells.

In one series of experiments, the researchers injected FLiCRE into cells in the nucleus accumbens and used an opsin to activate a neural pathway associated with avoidance behavior in the mice. Once the calcium in FLiCRE-containing cells spiked - the cellular indication that the mouse is avoiding something - the cells glowed a permanent red that was visible through a microscope. The researchers also sequenced the RNA of the cells to see which ones contained the fluorescent protein, producing a cell-by-cell record of neural activity.

"One goal was to map how brain regions are connected to each other in living animals, which is a really hard problem," said Christina Kim, a postdoctoral scholar in genetics at Stanford and co-lead author of the paper. "The beauty of FLiCRE is that we can pulse and activate neurons in one region and then record all of the connected downstream neurons. It is a really cool way to look at long-range brain activity connections."

In the next experiments, the researchers used the cellular activity map from the first experiments. They also adjusted FLiCRE so that the protein expressed the opsin protein, which can be controlled by orange light to alter neuronal activity. After activating FLiCRE in the cells, the researchers sent orange light through the fiber optic implant whenever the mice would enter a certain room. In response, the mice steered clear of that room, indicating that FLiCRE had indeed located cells in the brain that drive avoidant behavior.

A dream project

The development and testing of FLiCRE combined chemistry, genetics, biology and neuroscience, and many specialties within those disciplines. As a result, the tool has a wide range of possible applications, including in cells outside the brain, the researchers say.

"I moved to Stanford in 2016 with the hope of being able to carry out extremely interdisciplinary and collaborative projects such as this," said Ting. "This project has been one of the most rewarding aspects of my move to Stanford - seeing something this challenging and ambitious actually work out."

The researchers are now working on additional versions of FLiCRE, with a goal of streamlining the process. They are hoping to simplify its structure and also make it capable of working with other biochemical events, such as protein interactions or neurotransmitter release.

Reference: Kim CK, Sanchez MI, Hoerbelt P, et al.A Molecular Calcium Integrator Reveals a Striatal Cell Type Driving Aversion. Cell. 2020. doi:10.1016/j.cell.2020.11.015

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.

Visit link:
FLiCRE: A New Tool for Revealing the Brain in Action - Technology Networks

Neuroscience

Science Podcast Cohosted by BU Neuroscientist Brings Research to Listeners around the World – BU Today

Shen Ning and Mehdi Jorfi are on a mission to make cutting-edge medical research and impactful scientific discoveries accessible to everyone. After a year of hosting Science Rehashed, a podcast dedicated to the life sciences, they have become experts at dissecting, translating, and communicating meaningful science to students and young professionals across the globe who cant afford pay-for-access top-tier journals and peer-reviewed papers.

Many students outside of first-world countries have limited resources when it comes to accessing the most current scientific research and journals, says Ning, Science Rehashed cohost and a Boston University MD/PhD candidate studying neuroscience. Ning met Jorfi while collaborating on research at Massachusetts General Hospital (MGH) in 2019, when Jorfi approached her with the idea to start a science podcast.

While growing up and attending university in Iran, Jorfi had noticed the dearth of up-to-date research and resources in his local library, a big problem for an aspiring scientist. Ning, who grew up in China and moved to the United States at eight years old, also understood the challenges of being in a less resource-rich country, she says. Jorfi, now a faculty member at Harvard Medical School and MGH, believed creating a freely available podcast for young aspiring scientists abroad was a perfect way to fill this need.

Others quickly saw the value such a podcast would deliver, too. Within the first month of joining forces, Ning and Jorfi received funding from Innovate@BUs Innovation Pathway program, providing them with financial and creative support, as well as resources to communications mentors and trainers.

In 25- to 40-minute episodes, Ning and Jorfi have informal conversations with lead authors on high-impact papers published in peer-reviewed journals, releasing new episodes every three weeks. They have interviewed dozens of life scientists, ranging from biomedical engineers to physicians to biologists, and have even interviewed COVID-19 patients about their firsthand experiences. Episodes have garnered over 5,000 downloads, with listeners tuning in from over 75 countries. This year, their growing team of volunteer audio engineers and social media gurus won the 2020 Life Sciences & Healthcare Nonprofit Pitch Challenge, scoring them $2,500 to continue building and expanding their reach.

Im really happy that Ive been involved and have met amazing, incredible, inspiring people along my journey, says Ning. We have a wonderful team. Working on this on the side, in addition to your normal job or schooling, can be a lot. But with a really great, effective, complementary team, it doesnt feel like work at all.

The Brink caught up with Ning and Jorfi to hear how the podcast is going, what it takes to pull off a podcast, and how they communicate complex science effectively for a broad audience.

Q&AWith Shen Ning and Mehdi Jorfi

Jorfi: I sincerely believe that science and education is a right and not a privilege. But the reality is that many brilliant students and scientists worldwide, particularly those who live in emerging countries, start their journey at a disadvantage. I faced this reality while growing up in Iran attending university, where resources to top journals were scarce. The only resources available were outdated papers buried in the back of the stacks in the library from over 10 years ago. Years later, and many gray hairs later, I pursued my graduate degree in Switzerland and postdoctoral training at MIT and Harvard, where I was able to freely explore the latest science breakthroughs without any limitations. Because of this, I wanted to give back by helping future young scientists worldwide overcome the hurdles I experienced. One day I had an epiphany while listening to podcasts and reflecting back on my life, and Science Rehashed was born.

Ning: Mehdi approached me the next day and asked, what do you think about starting a science podcast? He explained his experience in Iran, and how itll be really helpful for people in those countries to have a resource that presents the most up-to-date science of the time around the world. And I was like, well, thats a really great point and a really great need we definitely need to address.

We started talking about our experience and strengthsI have a medicine background, with biology, neuroscience, and a little bit of bioengineering, and he has a background in biomedical engineering, chemistry, and also neuroscienceand we realized we can cover quite a few topics, but with a focus on life sciences and a touch of medicine. We wanted to choose papers that have a very high impact or have the potential to have a clinical or scientific impact in the upcoming 10 years. So, we started looking at top-notch journals, and we started inviting people. We recorded three episodes just to start off, and got it figured out. And then, we started working alongside Sofia Nastri, whos another BU student who graduated last year. Shes our lead audio engineer and shes a very talented scientist, too.

Ning: Yeah, we aimed pretty big in the beginning, just because one of the marketing strategies [for a podcast] is, once you get a few big people, then the rest follows.

Jorfi: There are millions of scientists and students in developing countries without the same resources as we have in Boston due to the lack of subscriptions to scientific journals at their institutions. Plus, we know how vital it is to sift through hundreds of scientific journals to stay up-to-date on the most pressing issues, yet a barrier of scientific jargon dilutes the main findings of studies.

Ning: With COVID-19, its becoming very apparent how important it is for the public to understand [research and the scientific process]. Before COVID-19, [the gene-editing technology known as] CRISPR was a big topic for public health and an ethical issueregarding [genetically engineered] babies and whether it should be legal or illegal. Especially with how quickly the life sciences are evolving and advancingalmost outpacing the ability for us to make the laws and policies [to regulate new technologies and therapeutics]its especially important.

Ning: Weve been working with a number of coaches who guide us to figure out how to create a narrative for each episode, and how to screen our guests so that the guests themselves are not difficult to understand in terms of jargon. Weve picked up a number of different communication skills, as well as story writing skills to include into our podcast. There are so many [specialized] words in science, abbreviations, and terminologies that for Mehdi and me, at this point, we dont even think about it. But, most people would be like, Okay, what are nucleotides? Lets talk about that! So, that has been a challenge, because sometimes we ask questions without initially framing [the subject] in a way that most people would understand. I go back and listen to some episodes and I can see how weve been improving. It takes weeks to go through the story, figure out which pieces we want to pull, whether we need to rerecord or explain jargon that may be very hard to understand for the general public. When youre listening to a podcast or music, sometimes you dont always pay attention 100 percent of the time, so its okay to repeat certain things that youve said before and, in fact, its probably encouraged. Weve been learning to rephrase things or ask the same question with different words. But coming from a science background, it has been nice because were able to have very natural conversations with the scientists. Anyone should be able to understand, if not the whole picture, at least lets say 70 to 80 percent of it.

One of our series, the 360 Perspective series, is really geared towards the general public and gives a holistic perspective on one topic or one particular public health concern. For the COVID-19 episode, we brought in patients, physicians from Italy, physicians in the United States, scientists who study COVID-19. And so we talked to them about their thoughts and their expertise, so the public can gain a better perspective of what was going on.

Jorfi: One of my favorite episodes is COVID-19: A 360 Perspective.

Ning: The first episode has a good introduction between me and Mehdi about the motivation behind the show, and it is an episode that was right up my alley. [The episode is] called the 40 Hertz Idea, with [our first guest] Dr. Li-Huei Tsai. Shes a leading female scientist in a field that is relatively male-dominated. Her work revolves around using 40 hertz of audio or visual stimulation to treat Alzheimers disease. I read [her] paper [describing the method] and followed the work over a number of years, so it was very exciting for me to hear the whole story from the person herself.

Ning: I think it would be really awesome to have Mayim Bialik from The Big Bang Theory, because she is a neuroscientist by training. That would be really cool as a part of our Wonder WomXn in Science & Engineering series that we just started.

Jorfi: We plan to continue to pursue our growth in the United States and United Kingdom, too, and we are aggressively targeting emerging countries with less available resources. Our goal is really to ensure that no aspiring individual will give up on science because of the lack of resources, because that should be the last challenge any scientists should have to face on their journey.

Ning: One of the things we did to increase our reach in the countries were targeting is that we started a Science Rehashed ambassador program, where anyonestudents, postdocs, early career scientistscan promote the podcast and introduce this resource to their peers and other people who might be looking for something like this. Right now, we have people from Iran, Peru, Switzerland, Turkey, Italy, Norway, India, and we have a US ambassador at Northeastern University. For some countries, there are language and cultural barriers that we need to overcome to capture our target audience. We are also focused on community outreach locally. So, getting involved with local youth programs and associations. Theres a huge number of organizations in Boston advocating for women in STEM and I think we want to join in that effort. Were looking into figuring out partnerships, either with other nonprofit organizations or venture capitals, biotech or pharma, that share the same type of mission or want to support us in our mission.

Interview has been edited and condensed for clarity.

View original post here:
Science Podcast Cohosted by BU Neuroscientist Brings Research to Listeners around the World - BU Today

Neuroscience

Cerevel Therapeutics to Host Inaugural Virtual R&D Event – GlobeNewswire

Event will focus on CVL-865, the Phase 2 GABA Positive Allosteric Modulator, and include an overview of key preclinical programs

Live webcast scheduled for Thursday, January 28 from 9:00 to 11:00 a.m. EST

CAMBRIDGE, Mass., Dec. 14, 2020 (GLOBE NEWSWIRE) -- Cerevel Therapeutics (Nasdaq: CERE), a company dedicated to unraveling the mysteries of the brain totreatneurosciencediseases, announced it will host the first in a series of virtual R&D events on Thursday, January 28 from 9:00 to 11:00 a.m. EST. Hosted as a live webcast, Cerevel will lead an in-depth discussion of CVL-865, its Phase 2 GABA positive allosteric modulator (GABA PAM) as well as provide an overview of leading preclinical / early clinical assets. Subsequent R&D events dedicated to additional portfolio programs will be announced at a future time.

CVL-865 is currently being studied in two clinical trials, including the Phase 2 REALIZE trial evaluating the compound as an adjunctive therapy in adults with drug-resistant focal onset seizures, and a Phase 1 proof-of-principle trial for acute anxiety in healthy volunteers. Data from the REALIZE trial are expected in the second half of 2022 and data from the Phase 1 trial for acute anxiety are expected in the second half of 2021. This event is intended to provide a detailed look at the science behind the current clinical program, with time for questions.

The live webcast can be accessed on the investor relations section of the Cerevel Therapeutics website here. A replay will be available in the same section of the companys website for approximately 90 days.

About CVL-865CVL-865 is a subtype selective positive allosteric modulator (PAM) that targets GABAA receptors containing 2/3/5 subunits. It is structurally differentiated from classical benzodiazepines and minimizes activity at 1-containing receptors, which is believed to help mitigate many of the adverse events associated with benzodiazepines. To date, CVL-865 has been evaluated in 289 patients and healthy volunteers across nine clinical trials, with results showing it to be generally well-tolerated. A Phase 2 single-dose trial demonstrated robust anticonvulsant activity in patients with photosensitive epilepsy (a type of epilepsy in which seizures are triggered by flashing lights), with six of seven patients treated with CVL-865 experiencing complete suppression of intermittent photic stimulation (IPS), a characteristic epileptiform discharge shown on electroencephalograms (EEGs). For more information about the Phase 2 clinical trial, please visit https://realizestudy.com.

About Cerevel TherapeuticsCerevel Therapeutics is dedicated to unraveling the mysteries of the brain to treat neuroscience diseases. The company is tackling neuroscience diseases with a differentiated approach that combines expertise in neurocircuitry with a focus on receptor selectivity. Cerevel Therapeutics has a diversified pipeline comprising five clinical-stage investigational therapies and several preclinical compounds with the potential to treat a range of neuroscience diseases, including schizophrenia, epilepsy, Parkinsons disease and substance use disorder. Headquartered in Cambridge, Mass., Cerevel Therapeutics is advancing its current research and development programs while exploring new modalities through internal research efforts, external collaborations or potential acquisitions. For more information, visit http://www.cerevel.com.

Special Note Regarding Forward-Looking StatementsThis press release contains forward-looking statements that are based on managements beliefs and assumptions and on information currently available to management. In some cases, you can identify forward-looking statements by the following words: may, will, could, would, should, expect, intend, plan, anticipate, believe, estimate, predict, project, potential, continue, ongoing or the negative of these terms or other comparable terminology, although not all forward-looking statements contain these words. These statements involve risks, uncertainties and other factors that may cause actual results, levels of activity, performance or achievements to be materially different from the information expressed or implied by these forward-looking statements. Although we believe that we have a reasonable basis for each forward-looking statement contained in this press release, we caution you that these statements are based on a combination of facts and factors currently known by us and our projections of the future, about which we cannot be certain. Forward-looking statements in this press release include, but are not limited to, statements about the potential attributes and benefits of our product candidates, the format and timing of our product development activities and clinical trials, including the expected timing of data announcements. We cannot assure you that the forward-looking statements in this press release will prove to be accurate. Furthermore, if the forward-looking statements prove to be inaccurate, the inaccuracy may be material. Actual performance and results may differ materially from those projected or suggested in the forward-looking statements due to various risks and uncertainties, including, among others: that clinical trial results may not be favorable; uncertainties inherent in the product development process (including with respect to the timing of results and whether such results will be predictive of future results); the impact of COVID-19 on the timing, progress and results of ongoing or planned clinical trials; other impacts of COVID-19, including operational disruptions or delays or to our ability to raise additional capital; whether and when, if at all, our product candidates will receive approval from the FDA or other regulatory authorities, and for which, if any, indications; competition from other biotechnology companies; uncertainties regarding intellectual property protection; and other risks identified in our SEC filings, including those under the heading Risk Factors in our definitive proxy statement/prospectus filed with the SEC on October 7, 2020. In light of the significant uncertainties in these forward-looking statements, you should not regard these statements as a representation or warranty by us or any other person that we will achieve our objectives and plans in any specified time frame, or at all. The forward-looking statements in this press release represent our views as of the date of this press release. We anticipate that subsequent events and developments will cause our views to change. However, while we may elect to update these forward-looking statements at some point in the future, we have no current intention of doing so except to the extent required by applicable law. You should, therefore, not rely on these forward-looking statements as representing our views as of any date subsequent to the date of this press release.

Media Contact:Rachel EidesW2O purereides@purecommunications.com

Investor Contact:Matthew CalistriCerevel Therapeuticsmatthew.calistri@cerevel.com

See more here:
Cerevel Therapeutics to Host Inaugural Virtual R&D Event - GlobeNewswire

Neuroscience

Global Neuroscience Antibodies and Assays Market 2020 | (COVID 19 Analysis) Offered In New Most recent Research Report with Forecast 2026 – The…

The globalNeuroscience Antibodies and Assaysmarket research report offers all the vital data in the domain. The latest report assists new bees as well as established market participants to analyze and predict the Neuroscience Antibodies and Assays market at the regional as well as global level. It covers the volume [k MT] as well as revenues [USD Million] of the global Neuroscience Antibodies and Assays market for the estimated period. Numerous key players BioLegend, Siemens, GenScript, Bio-Rad, Thermo Fisher Scientific, Rockland Immunochemicals, Abcam, Tecan, Merck KGaA, Santa Cruz Biotechnology, Cell Signaling Technology, F. Hoffmann-La Roche are dominating the global Neuroscience Antibodies and Assays market. These players hold the majority of share of the global Neuroscience Antibodies and Assays market.

Sample of global Neuroscience Antibodies and Assays Market Report at::https://www.marketresearchstore.com/report/global-neuroscience-antibodies-and-assays-market-report-2020-749830#RequestSample

The data presented in the global Neuroscience Antibodies and Assays market offers budding opportunities, which help users to make strategic moves and prosper their business. The report highlights the impact of numerous factors that might result in obstructing or propelling the Neuroscience Antibodies and Assays market at global as well as local level. The global Neuroscience Antibodies and Assays market research report offers the summary of key players dominating the market including several aspects such as their financial summary, business strategy, and most recent developments in these firms.

Key points of the global Neuroscience Antibodies and Assays market

Theoretical analysis of the global Neuroscience Antibodies and Assays market stimulators, products, and other vital facets Recent, historical, and future trends in terms of revenue and market dynamics are reported Pin-point analysis of the competitive market dynamics and investment structure is predicted to grow Future market trends, latest innovations, and various business strategies are reported Market dynamics include growth influencers, opportunities, threats, challenges, and other crucial facets

The global Neuroscience Antibodies and Assays market research report offers users with an all-inclusive package of market analysis that includes current market size, expansion rate, and value chain analysis. The global Neuroscience Antibodies and Assays market is segmented on a regional basis Europe, North America, Latin America, Asia Pacific, and Middle East & Africa as well. To offer a comprehensive view and competitive outlook of the global Neuroscience Antibodies and Assays market, our review team employs numerous methodological procedures, for instance, Porters five forces analysis.

Ask Any Kind of Query Before Purchasing the Report Here::https://www.marketresearchstore.com/report/global-neuroscience-antibodies-and-assays-market-report-2020-749830#InquiryForBuying

This research report includes the analysis of various Neuroscience Antibodies and Assays market segments {Consumables, Instruments}; {Pharmaceutical & Biotechnology Companies, Academic & Research Institutes, Hospitals & Diagnostic Centers}. The bifurcation of the global market is done based on its present and prospective inclinations. The regional bifurcation involves the present market scenario in the region along with the future projection of the global Neuroscience Antibodies and Assays market. The global market report offers an overview of expected market conditions due to changes in the technological, topographical, and economic elements.

Questions answered in the report include

1. What is the expected market size by the end of the forecast period?2. What are the major factors initiating the global Neuroscience Antibodies and Assays market growth?3. What are the latest developments and trending market strategies that are influencing the growth of the Neuroscience Antibodies and Assays market?4. What are the key outcomes of the Neuroscience Antibodies and Assays market developments?5. Who are the key players in the market?6. What are the opportunities and challenges faced by the key players?

Read Detailed Index of full Research Study at:https://www.marketresearchstore.com/report/global-neuroscience-antibodies-and-assays-market-report-2020-749830

Key Reasons for Purchasing Global Neuroscience Antibodies and Assays Market Report

New approaches and latest development trend that describe the structure of the market Advanced market breakdown structure Historical data and future market scope In-depth market analysis based on statistics, growth stimulators, and market developments Statistical data representation through figurative, numerical, and theoretical elaboration Report provides insight of the business and sales activities

Read more:
Global Neuroscience Antibodies and Assays Market 2020 | (COVID 19 Analysis) Offered In New Most recent Research Report with Forecast 2026 - The...

Anatomy

Soccer’s craziest kits: Meet the designer behind jerseys based on a tuxedo, human anatomy and The Hulk – ESPN

Remember the soccer team that went viral after they released a tuxedo-themed kit? Perhaps you saw the stir caused by a muscular uniform that made the players look like figures in a medical school textbook? Or even the club that fielded a team of players all dressed as The Hulk? Well, behind some of football's most infamous, bold and eye-catching outfits is a former player turned visionary kit designer.

His name is Juan Francisco Martin Fresneda, 49, a TV executive in Castilla y Leon, Spain, who dreamed up Cultural Leonesa's jersey that looks like a dinner jacket and bow-tie and Palencia's "skinless" human torso strip. Astorga's design that looked like Dr Bruce Banner post-transformation and Zamora's depiction of the human circulatory system are also of his creation. When they were released, the kits swept across social media and provided much-needed financial boosts for clubs well below Spain's glamorous elite.

- They wore that? Soccer's worst kits of all-time, ranked- Timeless classics: Ranking the greatest ever soccer kits

Martin is no stranger to the struggles of semi-professional teams. He played for several clubs in Spain's lower leagues such as his hometown side Gimnastica Segoviana, Melilla, Lorca and Murcia, even making it as high as the third-tier Segunda Division B. His breakthrough into the world of kit design came in 2014 when he was consulted by Cultural Leonesa, who were close to financial ruin and desperate for funding ideas.

"The designs of the kits were born out of necessity," Martin told ESPN. "Necessity is the mother of invention. I was the director of Leon TV in 2014 and Cultural was living a dramatic moment, they had big debts and were close to dissolving. I was collaborating with their board as a consultant."

Martin's background in marketing helped him come up with the out-of-the-box idea of the tuxedo jersey. The kit was an undisputed success, garnering the sort of worldwide attention that is rarely afforded to Spain's lower leagues, and from there other clubs came to him looking for advice.

"When someone is involved in the world of creativity, there is no limit, but you don't have to force it either," he says. "I have a couple of ideas but I have to wait for someone to understand and want them. We are in a project to go into basketball and there are other couple of things that I'm working on. I am convinced that in the near future all clubs -- including Barcelona, Real Madrid, Bayern Munich -- are going to have to create an original third kit to sell because we are in the world of survival. Football shirts are a way of telling stories.

"There are three types of shirts: the ones that are beautiful, made by Nike and Adidas; the ones that looks for publicity, like the one from Guijuelo [a Spanish club who gained fame for their jersey that appeared to be covered in ham]; and the ones that play with the emotions, which are the ones that I propose. I have the idea of the emotional shirt that I would design for Madrid and Barca and I am sure that it would sell millions of kits. It does not have to do with colours, but with emotions. I can't give clues because otherwise they would copy them from me ... Look, the Atletico Madrid fans are going to buy the Atletico shirt regardless of the design. But if you manage to make a shirt that tells stories ... and perhaps have an emotional tie to the club, the sales would multiply by 10."

Martin's relationship with football is born from personal tragedy, and his involvement in the game is a way for him to give something back.

"My life is a little bit like those you can see in the movies," he says. "When I was 17, my family died in a traffic accident and I was left alone with my father. It was a dramatic situation and football helped me a lot, it gave me the family that I did not have, a reason for life. I understood football as a school of values and I got into the world of football as a coach and also I was on the board of my hometown club, Gimnastica Segoviana."

Here is the story of Martin's unique kit designs, in his own words.

- ESPN+ viewer's guide: Bundesliga, Serie A, MLS, FA Cup and more- Stream ESPN FC Daily on ESPN+ (U.S. only)

Motto: "Cultural, the most elegant football team"

Background: "They had big debts and were close to dissolution. I was collaborating with their board as a consultant. It was the club's 90th anniversary and one night in our WhatsApp group I said I had half an idea which could change their fortunes. I asked: 'What do you think if Cultural come out wearing a tuxedo for their 90th birthday?' There were jokes, many did not see it as a good idea. I talked with Hummel [Cultural's kit manufacturer at the time] and at first they had doubts, but they offered me an idea. They showed me a design and to be honest it was very ugly. I didn't like it, but the idea worked. We made some minor changes. The idea was to adapt a black shirt and with a kind of sticker to make it look like a tuxedo. The final design wasn't much prettier, but it got the effect that I was looking for."

2 Related

Impact: "Within 24 hours there was an absolute avalanche of news all over the world. News on five continents. We went from having meetings about how to combat the debts to seeing which of the nine board members would be free to speak with Japanese or German TV. We sold between 4,000 and 5,000 of the kits. The problem was nobody anticipated such a success and Hummel was not prepared to meet the need. We could not supply all the requests. Remember, Cultural were in the third division and in a normal season only the 20 or 30 biggest fans used to buy the shirt.

"In 2014, we created this shirt and Qatari group Aspire bought the club the following year [when the club released updated home and away editions of their tuxedo kits]. And before them, other people came with an interest in sponsoring the club."

"There was some criticism -- there were people who said that the club lost their identity with that shirt. There was a moment when Cultural played against Real Madrid in the Copa del Rey [they lost 13-2 on aggregate]. I told them 'if you go out with the tuxedo shirt at the Bernabeu, with half the planet watching the game, you would sell 50,000 kits.' But the club prioritised their identity, and played with the original shirt."

Motto: "Leave the skin"

Background: "A colleague who I played with at Lorca called me and told me that Palencia had serious financial issues. They were leading the [fourth-tier Tercera Division] table but feared that they could not play the promotion playoff phase of the season because the players were not being paid.

"When I played there was a motto in the world of football, 'we have left our skin on the pitch' [a popular Spanish phrase meaning 'to give your all'], so the idea of muscles came up. It was difficult because the players did not believe. They were owed two months' salary, they were two months away from playing the promotion playoffs and demanded money or threatened to go on strike.

"I got one of the shirts sponsors, Ingenova, to invest some money after seeing the success of the Cultural Tuxedo kit. I told them that if they backed this idea, it will go out all over the world. They gave a loan to the club for all the money that was owed so that they could continue."

Impact: "We put on a great event to present the kit -- with the tuxedo we made a pathetic presentation, it was just a news conference. I learned from that, and with Palencia we made a spectacular presentation. By 12:15 p.m. we were trending, journalists from all over the world were calling, there were hundreds of requests. Kappa had made a prediction they'd sell 200 shirts and in just one hour they were all sold. Palencia were promoted to the Segunda Division B."

Motto: "The Incredible Astorga"

Background: "I wanted to make a second version of Palencia's muscle kit. I thought about something stronger, like The Hulk. I thought of teams in the area that played in green and I remembered Astorga. I met with the club president and made her a proposal. She told me it was interesting, but we needed to create a brand as we did with the others. So we came up with 'The Incredible Astorga,' referring to The Hulk.

"I emphasised to them the most important aspect was the unveiling, and we made a spectacular presentation at the Gaudi Palace. The presentation was better than the shirt! The shirt is arguably the weakest of the four in terms of identity, but the presentation was spectacular, with the players appearing by breaking through a wall."

Impact: "We started to have requests from Japan, China ... the big TV stations engulfed the small city of Astorga. It was impressive. Astorga played the promotion playoffs, but did not go up because they lost their final game."

- Order the jersey via Atletico Astorga's official website

Motto: "Blood, sweat and tears"

Background: "Zamora were in a drastic situation. They owed in the region of 700,000 and were in the third division. I knew some people who were willing to invest in a football club. They looked at Zamora and I told them a very nice kit would attract attention. I told them the kit would be called 'Blood, Sweat and Tears.' It is based on the heart of Zamora and the route that the blood makes around the body. I gave them a presentation and they liked it.

Impact: "It was a tremendous success. The best two [kits] in terms of impact were Cultural's tuxedo and the Zamora kit. Personally, I think this is the nicest. Zamora did not go up in the 2018-19 season but they managed to get some stability and were promoted to the Segunda Division B the following campaign.

"Somehow, all four clubs improved the following years. Cultural were in the third division and they were promoted twice and even played in the Segunda Division, Astorga played the playoffs and Palencia and Zamora were promoted."

- Order the jersey via Zamora CF's official website

Here is the original post:
Soccer's craziest kits: Meet the designer behind jerseys based on a tuxedo, human anatomy and The Hulk - ESPN

Anatomy

Anatomy Of A Governor – WUNC

What is the typical gubernatorial profile in North Carolina? Are there patterns in the background, upbringing or political composition of those who have occupied the Governor's Mansion during the last 50 years? And what might these patterns tell us about who could be chief executive going forward?

Guests: Gary Pearce, political writer and advisor to former Gov. Jim Hunt;Morgan Jackson, co-founder of the political consulting group Nexus Strategies

Here is the original post:
Anatomy Of A Governor - WUNC

Anatomy

‘Tenet’ | Anatomy of a Scene – The New York Times

new video loaded: Tenet | Anatomy of a Scene

transcript

transcript

This is Christopher Nolan. Im the director and writer of Tenet. Early in the film, the protagonist played by John David Washington and Neil played by Robert Pattinson, they travel to Mumbai. And theyre tasked with breaking into something thats fairly unique to Mumbai, these high rise houses. So its like a mansion that rather than being wide on the ground, its vertical in the air. These aerials are some of the first aerials that any film company has been allowed to shoot in Mumbai ever. And it was a lot of coordination with the government to allow that to happen. But its a spectacular city and it was something I was very keen to get into the texture of the film. The hardware used to do this stunt obviously stuff weve made up these winches and so forth. Its an arborist catapult putting the line over the rail there. And we spent a lot of time talking to the stunt guys about how they could do this, and they came up with the way of having John David and Rob as you see here actually launch into the air. Theres a cut there very invisible by Jen Lame my great editor to the stunt guys who then were launched the entire way up the building for real. No CG. And here we see John David Washington showing his physical prowess as the protagonist, and Rob with the slightly more loose character of Neil. A little less disciplined but similarly skilled. Were getting our first glimpse there of Dimple Kapadia a great Bollywood actress who has lent her talents to the film. Denzil Smith is a co-star there, hes also well known in India. We shot in a real house. The family who allowed us to shoot there were incredibly welcoming. A lot of this stuff would normally be done you do the interiors in a set, so forth. We tried to do it all right there overlooking the ocean in Mumbai. This is the setup really for the character. We want to show his ruthless side, we want to show his skill. But what John David is able to bring to this is a sort of generosity of spirit, its a very warm presence. So not just pure sort of cynicism. My friend, guns are never conducive to a productive negotiation. Im not the man they send to negotiate or the man they send to make deals. But I am the man people talk to. It was such fun to come and be a part of the Mumbai film industry for a few days. I mean we were able to work with the most amazing local crew there. To say anything about a client would violate the tenet he lives by. And at the end here, we have the reveal of Dimple Kapadia who plays Priya. It was a delight working with her, and getting to work in Mumbai on this film. Sanjay, make a drink for our guest, please.

Recent episodes in Anatomy of a Scene

Film directors walk viewers through one scene of their movies, showing the magic, motives and the mistakes from behind the camera.

Film directors walk viewers through one scene of their movies, showing the magic, motives and the mistakes from behind the camera.

Read more here:
'Tenet' | Anatomy of a Scene - The New York Times

Anatomy

The anatomy of a skateboard – SurferToday

However, a complete skateboard features a total of 12 different individual components. Each of these elements has a function and requires fine adjustments.

The most intricate and complex component is the skateboard truck.

Trucks hold the deck a few inches above the ground, sustain the skateboarder's weight, and are responsible for keeping the wheels rolling.

If you want to assemble your skateboard with select components, all you need is a good skate tool for tightening wheels and mounting hardware and adjusting the kingpin's height.

Let's take a detailed look at the skateboard anatomy.

The deck is the skateboard's wooden platform where the rider places his or her feet and to which other parts are attached.

They come in different sizes and shapes, but most popsicle-shaped skateboard decks feature a kick in the tail and the nose.

Skateboard decks have subtle differences that distinguish the front (the nose) from the rear (the tail), so they're not exactly symmetrical.

The deck also incorporates some kind of concave and a more or less gentle end-to-end curve - rocker or camber.

Generally speaking, a narrow skateboard is more responsive and flips easily but will also be less stable; a wide board provides stability but is more difficult to maneuver.

If you are buying your first skateboard, place different decks on the ground, and choose a size that is slightly narrower than the length of your feet.

In other words, when your heels are even with one side, your toes should stick off the other side by about an inch.

A standard deck is made from seven layers of 1/16-inch thick hard maple veneer laminated with PVA glue and pressed to a complex form.

Grip Tape

The grip tape is a top surface sandpaper-like sheet that helps the skateboarder to grip the skateboard.

Bolts

Trucks are attached to the deck by bolts.

There are two sets of four holes drilled into the deck. A total of eight bolts hold the trucks onto the board.

You can loosen them for easier turning, but make sure they're tight enough so that you cannot undo them with your fingers.

Trucks are the central and most complex part of a skateboard deck.

They feature the mechanics that allow the rider to skate around, turn, and get to the air.

A skateboard truck is made of steel and is exceptionally resistant. Often, it outlives all the other components of a skateboard.

The truck comprises several parts: the baseplate, the kingpin, the hanger, the bushings, and the axles.

A truck's width is determined by the length of its hanger (127-187 mm) and axle (193-254 mm).

In the end, and ideally, a rider wants the outside of the wheels slightly hidden inside both sides of the deck.

So, ultimately, the choice of a deck has an impact on the correct size of trucks for the board.

Baseplate

The baseplate is a flat and solid base metal plate featuring machine-drilled holes that mount to the skateboard's deck.

Kingpin

The kingpin is a large threaded pin - or large bolt - that sticks out of the baseplate.

Hanger

The hanger attaches to the other end of the kingpin.

It's a T-shaped metal component that takes all the beating. As a result, it is the heaviest and sturdiest part of a skateboard.

It is usually made of steel, but there are also lighter, more durable, and more expensive alloys available in the market.

The hanger houses the axles that stick out and incorporate both wheels.

Bushings

Each truck has two bushings, the elements that allow the skateboard to turn.

The bushings are two small rubber cups that pivot when the skateboarder leans left or right.

They are sandwiched between the baseplate and the hanger, i.e., the metal parts of any skateboard.

There's a bolt holding the bushings and hanger onto the kingpin that can be tightened or loosened to adjust how easily the board turns.

Bushings have a variety of hardness levels, depending on the riding conditions.

Technical and heavy riders often opt for stiffer bushings; cruisers and light skaters prefer softer bearings for easy turning.

Bushings may need to be replaced when they become worn out and start to crack.

Axle

The axles, also known as hanger shafts, connect the two wheels on each truck and are held in place by axle nuts.

They range from 193 to 254 mm and set the standard for how trucks are measured.

As a general rule of thumb, the axle length should put the wheels within one-quarter of an inch of the deck's edge.

Skateboard wheels should run smoothly on their axles.

Risers

The riser is an optional element that can be placed between the baseplate and the deck.

It's a plastic or rubbery pad that absorbs shock and offers extra cushion.

It may increase the wooden deck's life by protecting it from sudden and powerful impacts transferred through the wheels and trucks.

They also add space between the wheels and the bottom side of the deck to prevent the wheels from hitting the board's underside.

They make flip tricks difficult to land because they raise the skater's center of gravity.

A complete skateboard features four urethane wheels attached to the trucks and are categorized by diameter and hardness.

They range in size from 50 mm to 70 mm+ and have a hardness scale (durometer) that goes from 78A to 100A+.

Large and soft wheels are more forgiving and absorb impacts better. They're good for cruising and vert riding and range in size from 67 mm to 80 mm.

Small and hard wheels are fast and good for performing tricks and shredding skateparks and sidewalks. They range in size from 52 mm to 58 mm.

Axle Nuts

A complete skateboard uses four axle nuts that keep the wheels in place and spinning.

They tend to wear out quickly, so they should be replaced once in a while.

The standard size is 5/16'' for trucks that have 8 mm axles.

Washers

Most skateboards assembled in skate shops have two small washers between the nuts on the axle and between the hanger's inner bearings.

Washers help wheels spin smoothly.

Bearings

A complete skateboard has a total of eight bearings - two inside each of the four polyurethane wheels.

They look like rings with small steel balls inside.

Bearings fit into either side of the wheel and allow it to spin smoothly on the axle.

The most expensive bearings are ceramic bearings.

These high-performance bearings absorb the heat generated from the friction of a fast-spinning wheel and make the skateboard go fast.

However, precision steel bearings are the most common bearings used in skateboards.

Sometimes, there's also an aluminum spacer inside the wheel, between the bearings, that keeps everything aligned.

Bearings are rated for their ABEC (Annular Bearing Engineer's Committee) factor. That is to say they are rated for their quality and smoothness.

The ABEC scale ranges from 1 (low quality and cheap) to 9 (better quality and expensive).

View post:
The anatomy of a skateboard - SurferToday

Anatomy

Thursday Night Football Returns To Top Ratings; Greys Anatomy Paces ABC – Deadline

Foxs game pitting the Los Angeles Rams against the New England Patriots scored a night-leading 2.3 rating in the adults 18-49 demographic and 9.12 million viewers, returning to action after last weeks game was postponed because of positive Covid-19 tests.

The game, a 24-3 Rams win, was the first TNF tilt since November 19, an NFC West showdown between Seattle and Arizona. Last night was off three tenths in the demo from that Seahawks victory in early Nielsen Live+Same Day fast nationals, which because of the nature of live sports will likely see adjusted numbers later today.

ABCs full night of originals paced the scripted side last night, with Greys Anatomy (1.1, 5.50M) dipping a tenth from last week but still finishing as the nights top-rated non-sports program. Station 19 (0.8, 5.40M) was steady, while A Million Little Things (0.5, 5.34M)) grew a tenth.

NBC aired an all-specials lineup last night with the two-hour One Night Only: The Best of Broadway (0.5, 3.54M) followed by the reveal of the Time Person of the Year (0.3, 2.44M).

CBS was in repeats last night save for Star Trek: Discovery (0.2, 1.58M), even with last week. The CW re-aired its Silent Night A Song for the World special.

Follow this link:
Thursday Night Football Returns To Top Ratings; Greys Anatomy Paces ABC - Deadline