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What is Genetics? – Life Sciences Articles

Genetics is the study of heredity. Heredity is a biological process whereby a parent passes certain genes onto their children or offspring.

Every child inherits genes from both of their biological parents and these genes, in turn, express specific traits. Some of these traits may be physical for example hair and eye color etc.

On the other hand, some genes may also carry the risk of certain diseases and disorders that may be passed on from parents to their offspring.

Image Credit: fizkes/Shutterstock.com

The genetic information lies within the cell nucleus of each living cell in the body. The information can be considered to be retained in a book for example. Part of this book with the genetic information comes from the father while the other part comes from the mother.

The genes lie within the chromosomes. Humans have 23 pairs of these small thread-like structures in the nucleus of their cells. 23 or half of the total 46 comes from the mother while the other 23 comes from the father.

The chromosomes contain genes just like pages of a book. Some chromosomes may carry thousands of important genes while some may carry only a few.

The chromosomes, and therefore the genes, are made up of the chemical substance called DNA (DeoxyriboNucleic Acid). The chromosomes are very long thin strands of DNA, coiled up tightly.

At one point along their length, each chromosome has a constriction, called the centromere. The centromere divides the chromosomes into two arms: a long arm and a short arm.

Chromosomes are numbered from 1 to 22 and these are common for both sexes and called autosomes. There are also two chromosomes that have been given the letters X and Y and termed sex chromosomes. The X chromosome is much larger than the Y chromosome.

The genes are further made up of unique codes of chemical bases comprising of A, T, C and G (Adenine, Thymine, Cytosine, and Guanine). These chemical bases make up combinations with permutations and combinations. These are akin to the words on a page.

These chemical bases are part of the DNA. The words when strung together act as the blueprints that tell the cells of the body when and how to grow, mature and perform various functions. With age, the genes may be affected and may develop faults and damages due to environmental and endogenous toxins.

Women have 46 chromosomes (44 autosomes plus two copies of the X chromosome) in their body cells. They have half of this or 22 autosomes plus an X chromosome in their egg cells.

Men have 46 chromosomes (44 autosomes plus an X and a Y chromosome) in their body cells and have half of these 22 autosomes plus an X or Y chromosome in their sperm cells.

When the egg joins with the sperm, the resultant baby has 46 chromosomes (with either an XX in a female baby or XY in a male baby).

Each gene is a piece of genetic information. All the DNA in the cell makes up for the human genome. There are about 20,000 genes located on one of the 23 chromosome pairs found in the nucleus.

To date, about 12,800 genes have been mapped to specific locations (loci) on each of the chromosomes. This database was begun as part of the Human Genome Project. The project was officially completed in April 2003 but the exact number of genes in the human genome is still unknown.

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What is Genetics? - Life Sciences Articles

New study shows the importance of genetic testing in people with solid tumor cancers – News-Medical.Net

A new Cleveland Clinic study demonstrates the importance of genetics evaluation and genetic testing for children, adolescents and young adults with solid tumor cancers. The study was published today in Nature Communications.

Solid tumors account for half of cancer cases in children, adolescent and young adult (C-AYA) patients. The majority of these cases are assumed to result from germline variants (heritable changes affecting all cells in the body) rather than somatic alterations. However, little is known regarding the spectrum, frequency and implications of these germline variants.

In this study, led by Charis Eng, M.D., Ph.D., Cleveland Clinic's Genomic Medicine Institute, the researchers conducted the largest-to-date evaluation of germline mutations in C-AYA patients with solid tumors utilizing a combined dataset from Cleveland Clinic and St. Jude Children's Research Hospital.

Of the 1,507 patients analyzed, 12% carried germline pathogenic and/or likely pathogenic variants in known cancer-predisposing (KCPG) genes while an additional 61% had germline pathogenic variants in non-KCPG genes.

Our findings emphasize the necessity for all C-AYA patients with solid tumors to be sent for genetics evaluation and gene testing. Adult guidelines, particularly family history, are typically used to recognize C-AYA patients with possible heritable cancer, but studies have found a family history of cancer in only about 40% of patients with pathogenic and/or likely pathogenic variants."

Charis Eng, M.D., Ph.D., Genomic Medicine Institute,Cleveland Clinic's

The researchers also conducted a drug-target network analysis to determine if the pathogenic and/or likely pathogenic germline variants detected in the dataset were located within genes that could potentially be targeted by drug therapies.

Their analysis found that 511 (34%) patients had at least one pathogenic and/or likely pathogenic variant on a gene that is potentially druggable. Notably, they discovered that approximately one-third of these patients had variants that can be targeted by existing FDA-approved drugs.

"Currently, the majority of available targeted therapies are geared to adult patients, leaving few safe and effective treatment options for C-AYA patients," noted Dr. Eng. "However, we found that a significant number of the germline altered genes in C-AYA solid tumor cancers are targetable by FDA-approved drugs, which presents an opportunity to harness drug repurposing to identify therapeutic options for C-AYA patients."

Source:

Journal reference:

Akhavanfard, S., et al. (2020) Comprehensive germline genomic profiles of children, adolescents and young adults with solid tumors. Nature Communications. doi.org/10.1038/s41467-020-16067-1.

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New study shows the importance of genetic testing in people with solid tumor cancers - News-Medical.Net

Study: Genetics of AQP4 water channels impact the intensity of deep sleep – News-Medical.Net

The reason why we sleep remains an unresolved question of the 21st century. Research by Sara Marie Ulv Larsen, Sebastian Camillo Holst and colleagues from the Neurobiology Research Unit at the University Hospital Copenhagen, published this week in the open access journal PLoS Biology, now shows that the depth of non-rapid-eye-movement (nonREM) sleep in humans is associated with different genetic versions of a gene that encodes a water channel involved in fluid flow in the brain.

Recent insights suggest that sleep may enable and promote a flow of cerebrospinal fluid into the brain that literally removes metabolic waste. In experimental animals, this process is aided by water channels called AQP4; these form water-permeable pores through the cell membranes of brain cells called astrocytes.

The role of these water channels in the human brain and whether they are associated with the regulation of deep nonREM sleep, also called slow wave sleep, had not yet been examined.

A common set of genetic variants that are inherited together are called a haplotype. One such a haplotype (containing eight individual DNA variants) was previously shown to modulate the levels of AQP4.

By carefully studying more than 100 healthy individuals, the authors found that the depth of slow wave sleep, which can be measured by analyzing the brain waves recorded during sleep, differs between carriers of this haplotype and a control group.

The difference was most pronounced at the beginning of the night, when our need for sleep is highest. Interestingly, the two haplotype groups also coped differently when kept awake for two full days, suggesting that changes in the flow of fluids through AQP4 water channels may modify how we cope with sleep loss.

Because the genetic variants within the AQP4 haplotype were also previously associated with the progression of Alzheimer's disease, the results of this study may suggest that a sleep-driven exchange of fluids through AQP4 water channels could be linked to Alzheimer's progression.

To explore the possible association between Alzheimer's disease and AQP4 water channels, further studies are warranted.

The authors noted:

"A more immediate implication of our results is by improving our understanding of the importance of sleep"

In other words, this is the first study to show that the genetics of AQP4 water channels affect the intensity of deep sleep and how we cope with loss of sleep.

These findings add support to the current theory that sleep may be involved in the regulation of "brain clearance" and as such highlights the link between sleep and fluid flow in the human brain.

Source:

Journal reference:

Larsen, S. M. U., et al. (2020) Haplotype of the astrocytic water channel AQP4 is associated with slow wave energy regulation in human NREM sleep. PLOS Biology. doi.org/10.1371/journal.pbio.3000623.

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Study: Genetics of AQP4 water channels impact the intensity of deep sleep - News-Medical.Net

Breaking the Code: A lifetime of pain, due to genetic drug intolerance – FirstCoastNews.com WTLV-WJXX

JACKSONVILLE, Fla. For Linda Armstrong, the pain started 25 years ago with a truck going 45 miles an hour. It left her with severe neurological and brain injuries.

On a scale of one to 10, she said, There are days when its 10. I mean you literally cant move.

Like millions of Americans, she was prescribed opioids -- Vicodin, Dilaudid, Percocet but the pain persisted.

I would get some relief, but nothing that ever really made me functional, she said, "and it always made me nauseous.

Armstrongs lackluster response to pain medications had been a reality since she was a child. Once, getting a cavity filled, she recalls, I had to have 10 shots for one filling because it just would not numb.

Her resistance to drugs was simply anecdotal until she visited her doctor in October. Under new Centers for Disease Control and Prevention guidelines, doctors test opioid patients both for the presence of illegal drugs, and to ensure they are taking and not selling their pain meds.

Her test? Completely negative. There were no drugs of any type in my system.

Initially, her doctor wrote it off as a fluke.

When it happened a second time, Armstrong explained, her doctor said Linda we have to talk about this.

She took another test this time going so far as to take pills while her doctor watched. She even got her hair tested.

Still: Negative.

Fortunately, Armstrong has had the same doctor since the mid-'90s, and rather than doubting her, she worked to help get answers. She ordered a genetic test, which revealed significant abnormalities -- including in an enzyme called CYP2d6, which metabolizes opioids.

The test revealed something researchers in the field of pharmacogenomics know, but few patients do. People do not respond to drugs in the same way, explains Standford University researcher Dr. Russ Altman in a Ted Talk. Some people get no pain relief at all.

Armstrongs genetic test showed she was a hyper-metabolizer of opioids an issue that creates problems beyond pain. As Altman says, there are only two things a doctor can think when a patient complains they arent getting relief from opioids.

I could think this person is a CYPD2d6 non-responder, or I could think this is a trouble-making drug-seeker who wants something stronger, Altman said. Most doctors think the latter.

Armstrongs case is dramatic but not unusual. Dr Liewei Wang at Mayo Clinic in Rochester, Minn., is a leading researcher in pharmacogenomics research into the interaction of genes and drugs. She says 99 percent of people have at least one genetic variant that affects drug response. Response is variable, and in some cases not desirable.

Genetic testing can predict who may or may not respond, she said, and for those who dont respond -- are there alternative therapies?

In some cases, the wrong drug can be fatal. Already, hospitals like UF Health test cardiac patients before surgery for a gene that complicates blood clotting.

And some fields, like oncology and psychiatry, routinely use genetic tests to prescribe drugs. But most patients arent aware such tests even exist.

Jacksonville Pharmacist Todd Troxell believes genetic testing is the key to more effective prescribing. When you go get your glasses we dont just say, pick something up and go home. We get your prescription, he says. You cant see out of myglasses.

After looking at Armstrongs test, Troxell was able to steer her to a different pain medication that is working. I could tell from what she had on her report she wasnt going to be able to take certain medications.

Troxell would like to see genetic testing more widely available -- and comprehensible. Companies focused on translating dense genetic code into pharmaceutical recommendations were effectively shut down by the FDA in 2018. The agency worriedthe tests could pose significant public health concerns as inaccurate test results could impact the decision-making of healthcare providers and patients in ways that are seriously detrimental to patient health.

The agency walked that back slightly earlier this year, creating a Table of Pharmacogenetic Associations, which lists gene-drug interactions the agency believes are based on sufficient scientific evidence based on published literature.

For now, a genetic test needs to be ordered by a physician, who may or may not be able to interpret the code. And genetics remains just one element that doctors and patients should consider, including age, weight and environmental factors.

But patients like Armstrong say breaking the genetic code shouldnt be a secret it could literally spare them a lifetime of pain. Given the chance early on, she said, I would have had that test done in a heartbeat.

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Breaking the Code: A lifetime of pain, due to genetic drug intolerance - FirstCoastNews.com WTLV-WJXX

The COVID-19 Pandemic: White House Coronavirus Task Force to Shift Focus; Genetic Analysis Confirms Virus Started in 2019; and More – DocWire News

World News:

The world is closing in on yet another grim milestone as COVID-19 has infected almost 4 million people worldwide. Worldometer now reports 3.7 million confirmed cases, with the novel virus SARS-CoV-2 now responsible for the deaths of almost 260,000 people across the globe.

Outside of the US, the countries with the most confirmed cases are:

UK Scientist Steps Down After Breaking Lockdown Rules He Helped Develop

The United Kingdoms scientific adviser, who helped architect the UKs lockdown rules, resigned after he broke his own protocols. Professor Neil Ferguson, the leading epidemiologist who advised the British government on its COVID-19 response, stepped down after a story broke in The Telegraph that he allowed his reported lover to visit him at home, thus breaking lockdown orders. London police commented by saying: We remain committed to our role in supporting adherence to government guidance and have made it clear that our starting position is explaining the need to follow the regulations with anyone who is in breach in order to keep people safe and protect the National Health Service (NHS).

National News:

Wednesday, May 6, 2020

Over 70,000 US Deaths

COVID-19 has now killed over 72,000 people in the United States as of Wednesday, May 6. There are over 1.2 million confirmed infections. The US epicenter of New York now reports 330,139 confirmed cases and 25,436 deaths while second leading New Jersey now has 131,705 confirmed cases and 8,292 deaths.

Outside of NY and NJ, the US states with the most confirmed infections are:

President Donald Trump said on Wednesday that the White House coronavirus task force will continue working indefinitely, reversing an initial decision he made on Tuesday to phase out the team in the coming weeks. Trump said that the group, said by Vice President Mike Pence, has done a fantastic job of bringing together vast highly complex resources, such as ventilators, face masks and testing systems. Because of this success, the Task Force will continue on indefinitely with its focus on SAFETY & OPENING UP OUR COUNTRY AGAIN, the president tweeted. Trump added that, We may add or subtract peopleto it, as appropriate. The Task Force will also be very focused on Vaccines & Therapeutics. Thank you!

A new genetic analysis of SARS-CoV-2, the virus that causes the disease COVID-19, shows that coronavirus started circulating in people late last year, and spread extremely quickly after the first infection, according to a CNN report.. This dispels any rumors that the virus had been around earlier than 2019. The analysis also found no evidence that the virus is becoming more transmissible. The virus is changing, but this in itself does not mean its getting worse, genetics researcher Francois Balloux of the University College London Genetics Institute told CNN.

The US Center for Disease Control and Prevention says that at least 4,893 incarcerated or detained people have been infected with COVID-19. Moreover, the CDC reports that at least 2,778 staff members have tested positive for the respiratory disease, resulting in 15 deaths. Approximately one half of facilities with COVID-19 cases reported them among staff members but not among incarcerated persons, the report said. Because staff members move between correctional facilities and their communities daily, they might be an important source of virus introduction into facilities.

Tuesday, May 5, 2020

Monday, May 4, 2020

Friday, May 1, 2020

Friday, May 1, 2020

As most US states begin easing their lockdown restrictions, its apparent that the US is still not of the virus grip. There are now over 1.1 million confirmed cases in the US and over 64,000 deaths. The countrys epicenter, New York, now reports 310,839 confirmed cases and 24,069 deaths. The nations capital, Washington DC, has seen an uptick in cases over the past seven weeks, and reported 335 new cases on Friday its biggest single day rise.

The US states outside of New York with the highest confirmed cases remain:

Dr. Anthony Fauci said the Trump administration is ramping up efforts for developing a coronavirus vaccine, and one could be available by January, the NY Post reports. We want to go quickly, but we want to make sure its safe and its effective, Faucisaid on NBCs Today showon Thursday. I think that is doable if things fall in the right place.

The US Centers for Disease Control and Prevention how the US deals with the COVID-19 pandemic in the coming weeks will be critical to how the virus will evolve come the fall season. I do think that what we do this summer is going to be critical, said Dr. Ann Schuchat, CDC principal deputy director in an interview with JAMA Network. The more we intensify the testing and expand the public health capacity and assure that our hospital capacity and material to support the hospitals is adequate or has excess, the better were going to be in the fall.

The COVID-19 Pandemic: April Round-up

Heres a full wrap-up of COVID-19 happenings from the month of March.

The COVID-19 Pandemic March Round-up: Coronavirus Declared a Pandemic; People, Businesses and Economies Devastated

DocWire News will provide breaking updates on the COVID-19 pandemic as they become available.

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The COVID-19 Pandemic: White House Coronavirus Task Force to Shift Focus; Genetic Analysis Confirms Virus Started in 2019; and More - DocWire News

Fertility treatment and coronavirus: The Covid-19 baby boom jokes are hard to take – The Irish Times

The rest of the country only now have the experience of living with life on hold something Ive been dealing with for nearly three years, as well as my grief for my lost babies. This lockdown will end for most soon. Not so for us, says Elizabeth.

The emotional and psychological toll of undergoing fertility treatment is, she says, its own kind of lockdown.

In March, what Elizabeth describes as the lonely and heartbreaking wait for a baby was thrown into further uncertainty, when the European Society of Human Reproduction and Embryology (ESHRE) recommended that all fertility patients considering or planning treatment, even if they do not meet the diagnostic criteria for Covid-19 infection, should avoid becoming pregnant.

Patients whose treatment was already under way were advised to defer. But no such advice was given to those who did not need assisted reproductive treatment (ART) to become pregnant.

In March, the European Centre for Disease Control advised that there is no current evidence .. that infection with Covid-19 during pregnancy has a negative effect on the foetus no evidence of transmission of Covid-19 from mother to baby during pregnancy.

This caused distress and confusion for those undergoing treatment. I feel very discriminated against because there was no advice for normal women not to get pregnant, says Elizabeth.

Dr John Kennedy, medical director of the Sims Fertility Clinic in Dublin, says that the decision by Irish clinics to close was made in the national interest. Nobody actually formally directed us to close. But of course it is impossible to practice fertility treatment and maintain social distancing.

Fertility clinics were not originally classified as essential services. But the longer this goes on, the more essential we become, he says.

Last week, the ESHRE recommendations were updated to state that the pandemic is stabilising and the return to normal daily life will also see the need to restart the provision of ART treatments. The majority of Irish fertility clinics are planning to reopen from next week.

Even so, there remains a lot of uncertainty for the estimated 5,000-6,000 people who undergo fertility treatment in Ireland each year. Even before Elizabeths donor egg treatment was postponed, she had already experienced long delays including the nine months it took from her initial consultation to embryo transfer, a cycle which sadly ended in miscarriage.

At 43, she is acutely conscious of time passing. I havent had any updates from my clinic, just that theyll be contacting everyone. Im really hoping I can get started in June. Time goes by very slowly for women like me, and there can be many obstacles and delays.

The jokes about a Covid-19 baby boom are hard to take, she says.

Its time-sensitive, especially for the patients in their 40s. Its enough to make your mental wellbeing really poor, says Dr Bart Kuczera of the Beacon Care Fertility Clinic, which will also reopen next week.

But while the clinic will be open, he warns that you wont be able to start on Tuesday and have all the services available on Wednesday. Fertility cycles follow human reproductive cycles. So it will take at least a month for everything to be back.

For the clinics to reopen, major changes have had to be made in their operation, says Kennedy. Were completely changing how we work: longer days with less staff; theres going to be social distancing where possible for staff and patients; theres going to be appropriate use of personal protective equipment (PPE) which were in the process of sourcing.

Consultations will be virtual where possible, and some supportive drug treatments like immunotherapy and steroid treatment, wont be immediately available. All of the core things egg collection, ICSI, pre-implantation genetic screening will be proceeding as normal.

The early advice from ESHRE, that candidates for fertility treatment should avoid becoming pregnant, has left some women with lingering worries about potential risks. Every answer anybody gives has an asterisk beside it. We just dont know for sure. We think there arent any impacts unlike Sars and Mers, there doesnt appear to be an increased risk. It all appears safe, Kennedy says.

During recent virtual consultations, Kuczera noticed a sharp increase in the number of enquiries about egg freezing suggesting that some women were worried about a risk of becoming pregnant now, but wanted to try to protect their chances in the future. Half of the initial consultations were about egg freezing.

Even when the Irish clinics are once again up and running, for those had been undergoing treatment abroad, there are likely to be further delays.

Miriam travelled to Prague in the final days before international flights stopped so that she could undergo egg retrieval. She is very grateful for the care she received and has no regrets about her choice to undergo IVF there. But, she says, now we have no clue when we might get there to retrieve our embryos. It costs approximately 1,500 to have them shipped, and who knows the risks of that. I hope the governments of both countries recognise the trauma the ESHRE [recommendations have] caused to women like myself.

The Irish Times recently published a letter from another woman, Sarah K, describing the impact of the closure of clinics on her. Sarah K says now: Along with the universal uncertainty ... fertility patients have had to bear the added uncertainty as to whether this delay in treatment will affect their chance of becoming a parent. Im 42 and started this journey over two years ago. I never ever thought I would be still here, in this horrendous, anxiety-filled holding pattern.

As with everyone who spoke to The Irish Times, Sarah K is relieved at the updated ESHRE guidelines. Still, the waiting game goes on. Im still not sure as to when I will get to do my embryo transfer. I can only hope it is soon. Some might think it is only a few months delay, but for me every hour, day, month delay is an hour, a day and a month I wont have with my much longed-for child, and my parents wont have with their much sought after grandchild.

Names of those undergoing fertility treatment have been withheld to protect their privacy

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Fertility treatment and coronavirus: The Covid-19 baby boom jokes are hard to take - The Irish Times

What have couples been doing in lockdown? We might know in nine months – The Irish Times

Binge watching box sets, doing jigsaws and sharing almost every human activity possible through video apps are some of the most talked about ways of connecting and alleviating boredom during the coronavirus shutdown.

But what else are couples doing more of behind closed doors?

Maybe nine months will tell.

There has been plenty of light-hearted chatter predicting a wave of coronababies around Christmas and into 2021. A UK discount chain reported, with some glee, towards the end of April that sales of pregnancy test kits were up 25 per cent since the lockdown had begun in Britain on March 23rd.

However, while each pregnancy is ultimately the responsibility of just two people, whats going on in the wider society and economy have a huge bearing on a countrys birth rate. The current crisis is unprecedented and the ramifications are only starting to unfold.

We can only speculate on what impact the Covid-19 era might have, for better or for worse, on procreation in Ireland.

Here are some factors to consider:

At the outset, its important to understand the distinction between these two measurements when talking about increases and decreases in our baby population.

The fertility rate is the average number of children born to women of childbearing age (15-44), while the number of births is recorded as the number of live births per 1,000 of the population each year.

Here in Ireland, our birth numbers have shot up and down in recent decades while our fertility rate has slowly drifted downwards.

We had the highest birth rate in the EU in 2018, with 12.5 births per 1,000 residents while Italy recorded the lowest, at 7.3 per 1,000. However, this is primarily shaped by demographics (see below) rather than personal decision-making about whether or not to start, or enlarge, a family.

An EU comparison of fertility rates for 2017 showed Ireland had the third highest fertility rate, with 1.77 live births per woman, behind France, which topped the list with 1.90 live births per woman, and Sweden fractionally ahead of us with 1.78.

Sixty years ago, our fertility rate was 3.78 births per woman in 1960, the highest in Europe, and we held on to that top spot through 1970 and 1980 but by 1990 our fertility rate of 2.11 trailed that of Cyprus, Iceland and Sweden.

One of the biggest influences on Ireland in the past three decades has been migration, explains Prof Tony Fahey, Professor of Social Policy in UCD, and its effects are seen clearly in the maternity hospitals.

While the propensity of each woman to have children hasnt changed that much over the last 30 years, the number of children who have been born has changed enormously, he points out.

The Republics highest number of births in the 20th century was in 1980, at 74,064 according to CSO figures, and that fell to a low of just over 48,000 in 1994 reflecting the 1980s tide of emigration until a new peak of 75,554 births was reached in 2009.

The upsurge from the mid-1990s was driven entirely by the inflow of women in their late 20s, early 30s, so it was far more women having children, rather than women having more children, says Fahey. The tendency of the average woman to have children hasnt changed very much and has continued to be relatively high in Ireland.

In considering the impact of the coronavirus, he says its interesting to note that this pattern of childbearing was not affected much by the financial crisis in 2008 to 2012.

It did edge down a little, but you didnt get the kind of collapse you got in southern Europe and eastern Asia. We have never had a fall like that in Ireland but that could still happen.

Inward or outward migration, post-Covid-19, will depend not just on what happens to the economy in Ireland but also its relative performance to that of other countries.

We could have another bout of inward migration, or it could be flat, or, if recovery is stronger elsewhere sooner, we could have people leaving Ireland again, which would push the birth rate down again.

How was it for you? The change of life imposed by Covid-19 that is. Mixed anecdotal messages about increased or decreased sex drive could be broadly explained by individual responses to changes in circumstances and with whom youre sharing your home.

Just because many cohabiting couples have had more time and opportunity for sex, doesnt mean they wanted it. For some the novelty of change in routine, with perhaps more sleep, no commute and the relief of being removed from the communal workplace, may have been invigorating. For others, the heightened anxiety is paralysing; or maybe its the exhausting effect of ever-present children.

Stress has an impact on intimacy and desire, says Dublin psychotherapist and couples counsellor Bernadette Ryan, who points to the fear engendered by the pandemic. Suddenly, our bodies have become our enemy and that can bring a lot of anxieties up for people; those kinds of things impact on sexual intimacy.

Sex therapist Diane Gleim, writing on the effect of this time on libido, explains: A persons sex drive needs just enough anxiety/tension/uncertainty to get activated but not too much anxiety/tension/uncertainty or else the person can get overwhelmed, flooded, and then sex drive goes underground.

Its a spectrum, she says in Psychology Today, on which any one of us can move up and down, depending on how youre coping mentally with the pandemic fallout.

With short-term national emergencies in the recent past, such as the crippling snowfalls in February, 2018, courtesy of The Beast from the East, there was an air of excitement, says Ryan about battening down the hatches for a couple of days and that might heighten the sense of wanting to be intimate. This is different; this is endless and we dont know what the new normal is going to be.

Routines and distractions for all of us have radically changed, which alters the dynamics of every relationship. Sex tends to be the thing that goes on the back burner at times of stress, she says, and if it has for you, that is fine dont feel under pressure.

Ryan expects a post-Covid-19 surge in demand for counselling at her Ranelagh practice (dsixcounselling@gmail.com), fearing that the pressure of living with the restrictions will have exacerbated cracks couples might have had in their relationship.

For people cooped up with small children in small places, its tough, she adds. It really will test the mettle of the relationship.

Fertility experts say that couples often dont have sex frequently enough to give themselves the best chance of conceiving, with intercourse three or four times every week being recommended. If that level of sexual activity has been more attainable since mid-March for more couples, there would surely be an increased pregnancy rate among those who are not using contraception.

You can add to that a surmise that anecdotal accounts of more alcohol being consumed at home, along with trade reports of a 40 per cent rise in take-home alcohol sales on the same period last year, may have increased sexual risk-taking and faulty use of contraceptives.

Since were living through what amounts to a social experiment, there is no knowing whether the psychological impact would make couples less or more likely to think about starting a family or, indeed, enlarging one.

On the one hand there is huge uncertainty about what sort of world you might be bringing a child into in nine months time, Ryan points out, on the other hand people can realise through all this what is important for them in life. Those who might have been putting off starting a family, may now feel the time has come, she suggests.

Up to now, the number of Irish women going on to have a third or fourth child has tended to keep the birth rate up in Ireland, says Fahey. Often it is the middle-class and professional women who are having the third and fourth child, which really is a bit unusual in international terms.

While immigrant women have accounted for a sizeable share of births in Ireland some 25 per cent of births in 2012 were to non-Irish mothers they tend to have fewer children than Irish women, he says, as many of them come from Eastern Europe where birth rates are lower and family sizes smaller than in Ireland.

This likelihood of the third or fourth child is one thing that Fahey thinks may change, not just due to the Covid-19 economic fallout but other pre-existing issues, such as the housing crisis.

A standout example in recent times of the impact mass uncertainty can have on a countrys birth rate is what happened in East Germany over the period of reunification with West Germany.

It is probably the most astonishing case study we have, says Dr Jo Murphy-Lawless, research fellow at NUI Galways Centre for Health Evaluation, Methodology Research and Evidence Synthesis.

Between 1989, the year the Berlin Wall fell, and 1991, a year after reunification, the number of births in the old GDR fell by 44 per cent, she reports. The completed fertility rate was 1.57 in 1989; by 1991 it was 0.8.

It didnt really begin to recover at all, only slightly, until the mid-1990s. It was almost as if women in East German, seeing the collapse of Communism, just gave up having children because of the absolute lack of confidence in their immediate futures.

Murphy-Lawless had a masters student who had lived in East Germany and who did her thesis on the experience of women there making the transition to a united Germany. Her research highlighted how the former GDR had given significant support to women as child bearers and child rearers, says Murphy-Lawless.

Somehow that shock of having to recalibrate their lives led to this colossal drop in fertility, unlike anything we have ever had. In general, we have been floating down.

Yet, she adds, we still seem to have quite a fondness for children in Ireland. We have the highest percentage of households with children in them in the EU, which is 40 per cent.

The impact possible long-term travel restrictions post Covid-19 might have on peoples decisions on family formation is something that Murphy-Lawless also muses about.

Global consumerism capitalism, which has been part and parcel of individualisation, has meant people have just moved on to greener pastures, be it for economic, career or lifestyle reasons. How easy it is going to be to do that now is the great unknown, she suggests.

If the opportunity and/or social pressure to see the world before starting a family is curtailed, that may have an effect if more people choose to settle down in Ireland now. There may be fewer stories originating from this period of going away for a year and ending up staying away for a lifetime.

The housing problem is a factor in the margins that Fahey has been considering as having implications for our birth rate. The traditional three-bed semi-detached was suitable for a three- or four-child family where a two-bedroom apartment is not.

Having a little garden out the front and the back makes having children a lot easier, he suggests indeed, never more so than right now.

The difficult of acquiring a long-term home in the first instance may deter people from starting families at all, he says or at least delay them, with possible repercussions for fertility the longer they wait. An inability to trade up to a bigger property might also deter couples from having a third or fourth child.

They were the factors that were there and, oddly enough, the indications are that maybe the current crisis will ease the housing problem, he says, pointing, for example to Airbnb properties coming onto the rental and sales markets.

Also, if the lack of capacity in the construction sector has hindered the building of new houses, he wonders if the inevitable stalling of plans for new hotels in the Dublin area over the next few years might free up a workforce to accelerate the numbers of homes being built.

This is a roundabout way in which a crisis like this could have an upside effect rather than a downside effect, he adds.

The closure of assisted reproduction clinics since the last week of March was devastating for couples in the midst of, or about to embark on, treatment. They have lost months in what some regard as a race against time.

We didnt get an absolute direction to close, says Dr John Kennedy, medical director of theSimsDublin and Cork and the RotundaIVF clinics. We closed because it was the right thing to do.

The health services were under huge pressure, he points out. However, he had been a little surprised at the European Society of Human Reproduction and Embryology (ESHRE) advising, in a statement on March 19th, that, as a precautionary measure against possible negative effects of Covid-19, all fertility patients should avoid becoming pregnant.

On April 23rd, the ESHRE rowed back on that, advising that, as infertility is a disease and with the risk of Covid-19 decreasing, all ART treatments can be restarted for any clinical indication, in line with local regulations.

At the time of writing, Kennedy is in the midst of planning for reopening the first week of May, with a new way of working, including extended opening hours to allow for social distancing through segregated team working and space in patient waiting areas. However, he adds, everything comes with an asterisk, as we are in uncharted waters.

That goes to Prof Fahey who has written extensively over the years on demographics: As with anything in this field, there are so many factors piling in together and they are not all pointing in the one direction of what the overall outcome will be.

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What have couples been doing in lockdown? We might know in nine months - The Irish Times

Cooperative Extension adapts to keep you informed – The Robesonian

I think it is safe to say we are all experiencing things we have never faced before. So, how are you doing?

I bet everyone is asking you a similar question these days. When I am asked that question, I say things like, I will be glad when this is over, or This is crazy, but we will get through it, or Im fine. In my head I am thinking, I am not doing fine at all. This is not okay.

Like everyone else, I am staying home for those who might not fare so well if they were to be infected with COVID-19. Then I remind myself, I am doing this for the greater good! But it is stressful! Our response to the situation we are in has been so quick, we have had no time to process what has happened to our very ordinary lives.

Many of us are trying to work from home and manage our families at the same time. Parents and caregivers are now homeschool teachers for the children in their homes. We are all trying to help each other understand what is going on in our world and how we can adapt to what we are all calling our new normal.

Businesses in town are having the same struggles. Finding the new routine and rhythm they need to meet the needs of the community and still provide those services while keeping everyone safe is a challenge. North Carolina Cooperative Extension, Robeson County Center, is no different. We are working hard to find new ways to connect to our clients and continue to give the research-based information we are known to provide. We know that many in our community do not have internet capabilities to get information from the World Wide Web. It is challenging for some, and others may be afraid to try new things. As Christopher Robin said to Winnie the Pooh, Youre braver than you believe, stronger that you seem, and smarter than you think.

You can embrace these new challenges and master them to keep informed and in touch with the things and people that are important in your life. Robeson County Cooperative Extension is committed to staying in touch! We will continue to broaden our scope of how we provide information as well as continue to provide the hands-on approaches you need. We are very interested in knowing what information you need from us and how you want to receive that information. We will be reaching out in a variety of ways to get feedback. Please dont hesitate to let us know what you need from Extension. We will continue to add to and update the ways you can get information from us.

Currently, you can find us on Facebook with live segments, titled Digging in with Extension, that highlight gardening information using our raised beds as the demonstration areas. You may have tuned in to see the daily egg-citing embryology segments by 4-H over the past month. New ideas for youth programming are being generated as quickly as possible and information will be available in the near future. The research many farmers depend on in our county for making planting decisions is continuing, and you can look forward to new and different ways of see those results very soon.

Follow us on Facebook at North Carolina Cooperative Extension, Robeson County Center. You can also check our website at http://robeson.ces.ncsu.edu/ for informative articles, links to resources and information, and (once we can gather again safely) upcoming events.

For more information, contact Christy Strickland, County Extension director, at 910-671-3276, by email at [emailprotected], or visit our website at http://robeson.ces.ncsu.edu/.

Christy Strickland is the county Extension director with North Carolina Cooperative Extension, Robeson County Center. She can be reached at 910-671-3276, or at [emailprotected]

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Cooperative Extension adapts to keep you informed - The Robesonian

Coronavirus-hit mum gives birth to magic IVF baby six weeks premature in a hospital bereavement room – The Irish Sun

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A MUM battling coronavirus has given birth to a "magic" IVF baby six weeks premature in a hospital bereavement room.

Claire Trusson, 37, fell pregnant after having IVF treatment following two years of struggling to start a family with husband Murray Mitchell, 33.

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Just weeks before Claire was due, she started to experience cold-like symptoms which soon developed into a persistent cough.

She went into isolation, until she started experiencing contractions and was rushed to St Helier Hospital in Carshalton, near Sutton in South-West London.

While in hospital, medics had to put her in the most isolated room on the ward to keep her away from all others - which turned out to be the bereavement delivery room.

Claire went home and a day later tested positive for the virus - but found herself back in the bereavement suite just a week later on March 30 to safely give birth to baby Jake.

In the scheme of things, I am super lucky - I'm well, he's well and really it's amazing

The first-time mum said giving birth six weeks early while suffering from the virus was stressful, and she didn't expect to give birth in the bereavement room.

Claire said: "I found out this week that that's the bereavement room - that's where they put families with their stillborn babies so they can have some time with them.

"They have a memorial clock on the wall, and because I was timing my contractions when I was first in there, I spent a lot of time staring at that clock."

But she added that she was "really grateful" to have given birth to baby Jake and avoid "another six weeks of anxiety of what giving birth would look like".

She added: "In the scheme of things, I am super lucky - I'm well, he's well and really it's amazing."

What is IVF Treatment?

After struggling to conceive for two years, Claire and her husband received IVF on the NHS and a single egg was implanted in August 2019.

IVF is one of the most successful fertility treatments, and has given birth to 8 million babies worldwide.

The success rate is dependent on multiple factors, and range from 7 per cent to 29 per cent, according to the Human Fertilisation and Embryology Authority.

But despite her joy at having baby Jake, Claire is still concerned about passing the virus onto him due to a lack of face masks.

On the way home from hospital, Jake met his grandmother Angela and uncle David through the car window.

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She added: "Every little sneeze and every little cough and every little cry, I jump on him like, 'oh god, you've got coronavirus.'

"It sounds really reckless, but it's really hard to look after a baby and them not see your face - and I didn't really have any face masks."

"I'm just trying not to breathe on him."

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Coronavirus-hit mum gives birth to magic IVF baby six weeks premature in a hospital bereavement room - The Irish Sun

The Role of Microphysiological Systems for Oncology and Stem Cell Research – News-Medical.net

Sponsored Content by PittconMay 6 2020

In this interview, Dr. Nancy Allbritton from The Allbritton Lab talks to News-Medical and Life Sciences about the revolutionary technology and techniques for the application of new technologies for oncology and stem cell research.

Dr. Nancy Allbritton provides insight into the organ-on-a-chip and the ability it has to monitor and control the environment at the cellular and tissue level is one of the most promising applications for microengineered systems. Dr Allbritton will be presenting her research and findings at Pittcon 2020 in Chicago.

Microphysical systems seek to replicate the smallest functioning unit of an organ. For intestine-on-a-chip, it might be large or small intestinal crypts. For a heart, it may be a section of contractile muscle. For the liver, it may be a liver lobule or group of lobules. It's a group of interconnected cells so that they are not just behaving as a single cell or just a small cluster, but they began to show higher-order behavior and functioning.

I think one of the reasons microphysical systems are becoming increasingly important is that we've had a lot of breakthroughs in stem cell technology. The ability to grow human stem cells from different organs is now present, as well as the ability to create a tissue of differentiated cell types from primary stem cells or organ-specific cells from induced pluripotent stem cells.

Right now, it is the perfect time for this technology, because we have all the enabling microfabrication methods and stem cell biology innovations coming together. Another reason organs-on-chips are becoming important is the ability to grow human tissue. It's very hard to do experiments on the human population and get a good representation of the population, either because people aren't volunteering or because there just aren't enough people in that particular group of individuals. With the chip, you can begin to sample population-wide tissue variation. Organs on chips can also be a better way to test drugs. Rather than testing cells in a dish, which are usually tumor cells and very abnormal in their growth and other characteristics, organs-on-chips constructed from normal human cells can be used to get a more accurate representation of how drugs will affect humans. Additionally, they may outperform murine experiments in many ways since humans are not simply 70 kg mice.

We have been able to cure mice of all sorts of diseases, but much of this work hasnt translated to humans. So, while that was a good screening technology, a lot of drugs got through that were quite toxic to humans, and then a lot of drugs that may have worked well in humans, but were toxic to mice got blocked.

Image Credit:Shutterstock/royaltystockphoto.com

Microphysiologic devices should maximize the number of good drugs that could work well in humans. You can hopefully also get higher value information and create a much better drug pipeline. I think doing high-throughput screening on organ subunits on microfabricated devices can complement drug screening in humans. The idea is that you may be able to cut costs in several ways;

I believe you can also begin to develop human disease models. You can create mouse models of human disease, but they almost never completely recapitulate human disease. Even when it is a simple genetic mutation, the mice can often be asymptomatic or present with different symptoms and outcomes to that mutation. With the organ-on-a-chip, you can have functioning human tissue that mimics many of the symptoms of human diseases. Even more exciting, one can begin to put, for example, an intestine-on-chip coupled with the human gut microbiome or the gut flora and begin to understand how the tissue and microbiota interact.

This is important because our gut flora is very different from that of a mouse and other model organisms. We now know that intestinal microbes have a tremendous physiologic impact throughout our body including metabolism and mentation, our feeding behavior, and these are aspects of human behavior or physiology that can't be recapitulated in an animal model or cells in a dish.

Eventually, we'll be able to string human organs together as human organ-on-chip systems. For example, food is added to the gut model and absorbed, the nutrients travel to the liver, the liver metabolizes the absorbed compounds sending them out to the body as a whole including the brain and heart. The organs must be tied together to see the full effect in which the behavior or functioning of one organ impacts other connected organs. That will never replace a fully functioning human, but I think the expectation is that it may end up being a lot more accurate and, in some ways, cheaper than mice or other model mammalian systems.

The organ-on-a-chip systems will help us understand basic biology and the basic physiology of the human.

Microphysiologic devices depend on many fields of chemistry for their advancement including synthetic organic, polymer and analytical chemistry. We need more synthetic matrices to support cells and tissues including smart polymers and scaffolding materials that will support, direct and shape these organ systems. Right now, people often use matrices derived from native biologic materials, like collagen or matrigel, which are expensive and not fully defined. Polymer chemists and synthetic chemists are working hard to develop novel materials and matrices while analytical chemists, as well as engineers, are developing materials and methods for microdevice fabrication, sensor enhancements, and other device-related innovations.

Microphysiologic systems will clearly need an array of embedded and external sensors to monitor their health and well-being as well as pathophysiologic attributes. They will need robust, reliable (and often miniaturized) embedded sensors for oxygen consumption, glucose concentration, CO2 production, pH, and other chemical and physical attributes. Importantly the sensors should not perturb the system. These sensors will likely require external instrumentation or detection methods to monitor the embedded sensors, for example, RFID or optical readouts.

For engineering and chemistry, these are going to be huge areas where many can contribute to moving the field forward. For engineering, specifically, developing ways to make integrated systems that are efficient, low cost, manufacturable, shippable, self-contained, and can talk to each other will be important. This is an area where engineering and chemistry must work together in a team approach to move the field forward. It's going to take both disciplines to really advance the field.

Microfabricated technology refers to devices developed with micron-sized features. Cells have diameters on the order of 10 microns and many organ subunits span hundreds of microns so that micron-sized architectural features are required to recapitulate key features. For example, large intestinal crypts (the key physiologic subunit of the large intestine) is approximately 400 microns in length and 100 microns wide with the stem cell niche spanning tens of microns. Thus, microfabrication methods are perfect for rebuilding many of the key architectural features of the large intestine.

When it comes to rebuilding architectural features such as the lare intestine, microfabrication methods are perfect for this. Image Credit:Shutterstock/nobeastsofierece

We do intestine-on-a-chip, which is mostly the large intestine and have several platforms of varying complexity. The simplest is the human intestinal epithelial monolayers that have a stem and/or differentiated cells. These can be used to assess how the intestine transports and metabolizes drugs and nutrients or how the stem cells differentiate into mucus-producing, hormone-producing, or absorptive cell types. These systems are designed to be higher throughput model systems that are simple but do not possess the greatest possible information content. However, they are robust and reliable. We also have complex 3D tissues that replicate a wide range of physiologic behaviors as well as the architectural features of the human intestine. Importantly many of these systems will support the wide array of chemical and gas gradients found in the human intestine. They can also host the human microbiome so that a better understanding of the complex interplay between the human cells and microbes can be developed in both health and disease.

As with all of our model systems, we usually advise users of our systems to employ the simplest platform possible and then add in complexity as needed for the task at hand.

We have more complex platforms that are designed to replicate a lot of the different features of the human intestine, such as the architecture, cell migration behaviors, and stem cell fate decisions. These are shaped three-dimensional systems that exist as arrays of crypts (or microwells) covered with a monolayer of intestinal epithelial cells. The crypt array has a basal surface for a nutrient diffusion, and a lumen, like the inside of the intestine.

You can imagine the cells on the inside of the intestine, facing the waste or food, are very different from the cells that are at the base of the intestinal crypts. In these systems, the stem cells are found at the crypt or microwell base while the differentiated, mature cells such as absorptive cells are found facing the luminal surface, i.e. waste side. These systems are chemically and architecturally much more faithful duplicates of the human intestine than the simpler systems, but that also means they're a little harder to create, build, and maintain. With some of these three-dimensional systems, we create chemical gradients across the tissues, i.e. the long axis of the intestinal crypt, just as stem cell factors and food/bacterial metabolites exists in a gradient across your intestinal crypts. This microdevice permits very sophisticated micro-environmental control of these complex tissues.

We can do a lot of it, but we can't do it all. For example, with the chemical gradients, the stem cells that sit at the base of these microwells are exposed to very high growth factors, but about 400 microns away, so a few hair diameters, the cells don't see much of these factors. This chemical gradient mimics the signaling microenvironment of the intestine very closely. There are also all sorts of bacterial metabolites and products that are at very high concentration for the cells at the luminal surface, but low where the stem cells are down at the base of the microwells or crypts. We can also replicate these food and bacterial metabolite gradients by using purified compounds or actual bacteria on the luminal side of the intestine on a chip.

This 3D system begins to duplicate the human physiologic signals that control the cells behavior, but there are some things we cannot do yet. We don't have a blood supply, for example, or blood vessels going into our chip. Thats one of the next steps, but there is a lot of work to do to build it, yet still have a robust and reliable intestine-on-chip system. Our tissue is growing in complexity, but we still only have 1 to 2 tissues on the devices and a small subset of the bacteria found in the human intestine. One could imagine increasingly putting more and more tissue types. The intestine has epithelial cells, fibroblasts, muscle cells, neural cells, immune cells, all sorts of other cell types, and adding in all of those cell types would create a more normal physiologic organ. I think those are all goals for the future on which we will be moving forward.

Our intestinal-on-chip technologies can be used to understand drug transport and metabolism by humans (as opposed to mice or tissue-cultured tumor cells). Additionally, the microbes in the human intestine or the human intestinal epithelium can convert drugs into their active form or into a toxic metabolite. Along that line, one could begin to do rapid screens as to how drugs are modified, metabolized and transported across the intestinal epithelium. Importantly many drugs cause intestinal dysfunction or side effects and screens can be performed for the impact of the drugs on the intestinal cells themselves and the impact on intestinal barrier function, e.g. leakiness.

As an example, it's now also clear that in cancer chemotherapy, the gut and the bacteria within the gut play a huge role in how well the chemotherapy is working. How this works is not well understood, so I think there's going to be a big push to use these systems to understand how we can make anti-cancer drugs that are more effective and less toxic on the gut and other biological systems. We are only beginning to scratch the surface.

Image Credit:Shutterstock/ nobeastsofierce

I dont think microphysiological systems can ever totally replace animal models. It's the law in the US to test drugs on animals. We are also a long way off from having a full human-on-a-chip with all of the different organ systems in place and interconnected, whereas a mouse or other animal system is already there. I think it's more likely that the chips will reduce the number of animals used so that you can get higher value information, thereby complementing rather than replacing animal models. The chips will also enable insights as to how humans might ultimately respond differently from animal model systems.

On-going work in the organ-on-chip area is to demonstrate that these devices actually mimic and replicate human responses (and many have already been shown to mimic human physiology when animal models failed). Overall the future for organ-on-chip technologies appears quite bright and will undoubtedly grow in future importance and impact.

There are already devices that demonstrate many of the complex disease phenotypes. For example, blood vessels on-chip have replicated blood vessel diseases such as atherosclerosis and tumor metastases. There are some amazing heart-on-chip devices with functioning cardiac tissue subunits that accurately recapitulate the impact of cardiovascular drugs, so I think we are making good progress. I think that we will see a growing number of impressive and highly predictive disease models as time goes by.

Near term, our big goal is to really make an intestine-on-a-chip that fully replicates the human small and large intestines. We're working hard at putting a human microbiome or normal intestinal bacteria on our intestine-on-chip. In addition to chemical gradients, gas gradients such as oxygen exist across the intestinal crypts which we're working hard at replicating. We really want to add in more tissue types, such as the immune system, fibroblasts, and nervous tissue. We've got quite a bit of work to do to make a fully functioning replica so we are also starting to team up with other people, particularly liver-on-a-chip people so that our intestine-on-a-chip can absorb food and then send it to their liver-on-chip to create a fully functioning liver-intestinal model to recapitulate food digestion, metabolism, and detoxification.

A big part of our work is to make the systems robust and reliable as well as easy to use for biologists and clinical investigators. That may sound trivial, but it's not. A lot of great devices remain isolated in the inventors' lab due to their high degree of complexity. When biologists try to use these complex devices, there are just too many failure points. Even to make one of these systems shippable will be a challenge, because how do you give it to FedEx, yet arrive in good condition across the world? There are a lot of challenges with scalability, manufacturing, robustness, and reliability that my lab, in particular, is interested in tackling to make sure that these organ-on-chips get out into the real world and fulfill their potential.

To find out more please visit http://allbritton.web.unc.edu/

Dr. Allbritton is a Professor in the Department of Bioengineering and the Frank & Julie Jungers Dean of Engineering at the University of Washington in Seattle. She has been the scientific founder of four companies and enjoys international travel.

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The Role of Microphysiological Systems for Oncology and Stem Cell Research - News-Medical.net